Infrared nanospectroscopic mapping of a single metaphase chromosome

2019
journal article
article
20
cris.lastimport.wos2024-04-09T21:16:41Z
dc.abstract.enThe integrity of the chromatin structure is essential to every process occurring within eukaryotic nuclei. However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. Here, we have applied infrared nanospectroscopy (AFM-IR) to demonstrate molecular difference between eu- and heterochromatin and generate infrared maps of single metaphase chromosomes revealing detailed information on their molecular composition, with nanometric lateral spatial resolution. AFM-IR coupled with principal component analysis has confirmed that chromosome areas containing euchromatin and heterochromatin are distinguishable based on differences in the degree of methylation. AFM-IR distribution of eu- and heterochromatin was compared to standard fluorescent staining. We demonstrate the ability of our methodology to locate spatially the presence of anticancer drug sites in metaphase chromosomes and cellular nuclei. We show that the anticancer 'rule breaker' platinum compound [Pt[N(p-HC_{6}F_{4})CH_{2}]_{2}py_{2}] preferentially binds to heterochromatin, forming localized discrete foci due to condensation of DNA interacting with the drug. Given the importance of DNA methylation in the development of nearly all types of cancer, there is potential for infrared nanospectroscopy to be used to detect gene expression/suppression sites in the whole genome and to become an early screening tool for malignancy.pl
dc.affiliationWydział Farmaceutyczny : Zakład Biofizyki Farmaceutycznejpl
dc.cm.id95209
dc.contributor.authorLipiec, Ewelina - 103664 pl
dc.contributor.authorRuggeri, Francesco S.pl
dc.contributor.authorBenadiba, Carinepl
dc.contributor.authorBorkowska, Anna M.pl
dc.contributor.authorKobierski, Jan - 103663 pl
dc.contributor.authorMiszczyk, Justynapl
dc.contributor.authorWood, Bayden R.pl
dc.contributor.authorDeacon, Glen B.pl
dc.contributor.authorKulik, Andrzejpl
dc.contributor.authorDietler, Giovannipl
dc.contributor.authorKwiatek, Wojciech M.pl
dc.date.accessioned2019-11-07T09:36:57Z
dc.date.available2019-11-07T09:36:57Z
dc.date.issued2019pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number18pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume47pl
dc.identifier.articleide108pl
dc.identifier.doi10.1093/nar/gkz630pl
dc.identifier.eissn1362-4962pl
dc.identifier.issn0305-1048pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/86697
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeinne
dc.subtypeArticlepl
dc.titleInfrared nanospectroscopic mapping of a single metaphase chromosomepl
dc.title.journalNucleic Acids Researchpl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T21:16:41Z
dc.abstract.enpl
The integrity of the chromatin structure is essential to every process occurring within eukaryotic nuclei. However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. Here, we have applied infrared nanospectroscopy (AFM-IR) to demonstrate molecular difference between eu- and heterochromatin and generate infrared maps of single metaphase chromosomes revealing detailed information on their molecular composition, with nanometric lateral spatial resolution. AFM-IR coupled with principal component analysis has confirmed that chromosome areas containing euchromatin and heterochromatin are distinguishable based on differences in the degree of methylation. AFM-IR distribution of eu- and heterochromatin was compared to standard fluorescent staining. We demonstrate the ability of our methodology to locate spatially the presence of anticancer drug sites in metaphase chromosomes and cellular nuclei. We show that the anticancer 'rule breaker' platinum compound [Pt[N(p-HC_{6}F_{4})CH_{2}]_{2}py_{2}] preferentially binds to heterochromatin, forming localized discrete foci due to condensation of DNA interacting with the drug. Given the importance of DNA methylation in the development of nearly all types of cancer, there is potential for infrared nanospectroscopy to be used to detect gene expression/suppression sites in the whole genome and to become an early screening tool for malignancy.
dc.affiliationpl
Wydział Farmaceutyczny : Zakład Biofizyki Farmaceutycznej
dc.cm.id
95209
dc.contributor.authorpl
Lipiec, Ewelina - 103664
dc.contributor.authorpl
Ruggeri, Francesco S.
dc.contributor.authorpl
Benadiba, Carine
dc.contributor.authorpl
Borkowska, Anna M.
dc.contributor.authorpl
Kobierski, Jan - 103663
dc.contributor.authorpl
Miszczyk, Justyna
dc.contributor.authorpl
Wood, Bayden R.
dc.contributor.authorpl
Deacon, Glen B.
dc.contributor.authorpl
Kulik, Andrzej
dc.contributor.authorpl
Dietler, Giovanni
dc.contributor.authorpl
Kwiatek, Wojciech M.
dc.date.accessioned
2019-11-07T09:36:57Z
dc.date.available
2019-11-07T09:36:57Z
dc.date.issuedpl
2019
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
18
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
47
dc.identifier.articleidpl
e108
dc.identifier.doipl
10.1093/nar/gkz630
dc.identifier.eissnpl
1362-4962
dc.identifier.issnpl
0305-1048
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/86697
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
inne
dc.subtypepl
Article
dc.titlepl
Infrared nanospectroscopic mapping of a single metaphase chromosome
dc.title.journalpl
Nucleic Acids Research
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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