Infrared nanospectroscopic mapping of a single metaphase chromosome

doi:10.1093/nar/gkz630

Infrared nanospectroscopic mapping of a single ...

Bibliographic description

punktacja MNiSW [2019]: 200

title:	Infrared nanospectroscopic mapping of a single metaphase chromosome
author:	Lipiec Ewelina , Ruggeri Francesco S., Benadiba Carine, Borkowska Anna M., Kobierski Jan , Miszczyk Justyna, Wood Bayden R., Deacon Glen B., Kulik Andrzej, Dietler Giovanni, Kwiatek Wojciech M.
Journal title:	Nucleic Acids Research
volume:	47
issue:	18
date of publication:	2019
article ID:	e108
ISSN:	0305-1048
eISSN:	1362-4962
DOI:	10.1093/nar/gkz630
language:	English
Journal language:	English
abstract in english:	The integrity of the chromatin structure is essential to every process occurring within eukaryotic nuclei. However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. Here, we have applied infrared nanospectroscopy (AFM-IR) to demonstrate molecular difference between eu- and heterochromatin and generate infrared maps of single metaphase chromosomes revealing detailed information on their molecular composition, with nanometric lateral spatial resolution. AFM-IR coupled with principal component analysis has confirmed that chromosome areas containing euchromatin and heterochromatin are distinguishable based on differences in the degree of methylation. AFM-IR distribution of eu- and heterochromatin was compared to standard fluorescent staining. We demonstrate the ability of our methodology to locate spatially the presence of anticancer drug sites in metaphase chromosomes and cellular nuclei. We show that the anticancer 'rule breaker' platinum compound [Pt[N(p-HC_{6}F_{4})CH_{2}]_{2}py_{2}] preferentially binds to heterochromatin, forming localized discrete foci due to condensation of DNA interacting with the drug. Given the importance of DNA methylation in the development of nearly all types of cancer, there is potential for infrared nanospectroscopy to be used to detect gene expression/suppression sites in the whole genome and to become an early screening tool for malignancy.
affiliation:	Wydział Farmaceutyczny : Zakład Biofizyki Farmaceutycznej
type:	journal article
subtype:	academic paper