Physicochemical and micro-tomographic characterization of inorganic deposits associated with aortic stenosis

2014
journal article
article
dc.abstract.enBackground and the aim of the study: A major feature of aortic stenosis is massive mineralization of the aortic valve, though the mechanism of the process remains unclear. The study aim was to characterize the chemical composition and morphology of inorganic deposits from surgically excised natural aortic valves and to seek similarities to minerals in bones and teeth. Methods: Mineral deposits from 30 surgically excised natural aortic valves were examined. The control group consisted of autopsy samples (aortic valves, vertebral bodies) and teeth obtained after extraction. Micro-computed tomography \left ( \mu CT \right ) was used to describe the morphology and density of the minerals. X-ray fluorescence and Fourier transform infrared spectroscopy were applied to determine the chemical composition. Results: A poorly crystalline, B-type carbonate-containing hydroxyapatite (HAP) was found to constitute the mineral phase of the aortic valve leaflets. No other chemical compounds were identified. The elemental composition of the minerals in aortic valves and bone/tooth did not differ markedly, except that the Mg concentration was fourfold higher in valve material. The aortic valve deposits were irregular in shape and occupied ca. 40% of the leaflet volume. The volume of the deposits were spread over a broad range \left ( 0.001-0.03 mm^{3} \right ), while the density ranged from that of dentine to enamel \left ( average value 2.2 g/cm^{3} \right ), slightly higher than that of compact bone. Conclusion: The aortic valve deposits were identified as B-type carbonate-containing HAP, and were not identical to those found in bones and teeth, the main difference being a fourfold elevated Mg content. Mg may have been deposited as a separate compound, as \mu -CT results suggested that the formation of mineral deposits in aortic valves was a multi-factorial process. The morphological parameters and densities of the valve deposits were spread over a broad range \left ( factor \sim 300 \right ). An unequivocal identification of the mechanism responsible for the aortic valve pathological calcification was not possible, however.pl
dc.affiliationWydział Lekarski : Instytut Kardiologiipl
dc.affiliationWydział Lekarski : Zakład Anatomiipl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiegopl
dc.affiliationWydział Lekarski : Zakład Biofizykipl
dc.cm.id65989
dc.contributor.authorOrzechowska, Sylwia - 108746 pl
dc.contributor.authorWróbel, Andrzej - 132782 pl
dc.contributor.authorGoncerz, Grzegorz - 129557 pl
dc.contributor.authorPodolec, Piotr - 133185 pl
dc.contributor.authorRokita, Eugeniusz - 133291 pl
dc.date.accessioned2015-05-26T14:09:21Z
dc.date.available2015-05-26T14:09:21Z
dc.date.issued2014pl
dc.description.adminRokita, Eugeniusz [SAP20001931] - druga afiliacja autorapl
dc.description.number1pl
dc.description.physical40-47pl
dc.description.publication0,5pl
dc.description.volume23pl
dc.identifier.eissn2053-2644pl
dc.identifier.issn0966-8519pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/8157
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licenceBez licencji otwartego dostępu
dc.rights.uri*
dc.subtypeArticlepl
dc.titlePhysicochemical and micro-tomographic characterization of inorganic deposits associated with aortic stenosispl
dc.title.journalThe Journal of Heart Valve Diseasepl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Background and the aim of the study: A major feature of aortic stenosis is massive mineralization of the aortic valve, though the mechanism of the process remains unclear. The study aim was to characterize the chemical composition and morphology of inorganic deposits from surgically excised natural aortic valves and to seek similarities to minerals in bones and teeth. Methods: Mineral deposits from 30 surgically excised natural aortic valves were examined. The control group consisted of autopsy samples (aortic valves, vertebral bodies) and teeth obtained after extraction. Micro-computed tomography \left ( \mu CT \right ) was used to describe the morphology and density of the minerals. X-ray fluorescence and Fourier transform infrared spectroscopy were applied to determine the chemical composition. Results: A poorly crystalline, B-type carbonate-containing hydroxyapatite (HAP) was found to constitute the mineral phase of the aortic valve leaflets. No other chemical compounds were identified. The elemental composition of the minerals in aortic valves and bone/tooth did not differ markedly, except that the Mg concentration was fourfold higher in valve material. The aortic valve deposits were irregular in shape and occupied ca. 40% of the leaflet volume. The volume of the deposits were spread over a broad range \left ( 0.001-0.03 mm^{3} \right ), while the density ranged from that of dentine to enamel \left ( average value 2.2 g/cm^{3} \right ), slightly higher than that of compact bone. Conclusion: The aortic valve deposits were identified as B-type carbonate-containing HAP, and were not identical to those found in bones and teeth, the main difference being a fourfold elevated Mg content. Mg may have been deposited as a separate compound, as \mu -CT results suggested that the formation of mineral deposits in aortic valves was a multi-factorial process. The morphological parameters and densities of the valve deposits were spread over a broad range \left ( factor \sim 300 \right ). An unequivocal identification of the mechanism responsible for the aortic valve pathological calcification was not possible, however.
dc.affiliationpl
Wydział Lekarski : Instytut Kardiologii
dc.affiliationpl
Wydział Lekarski : Zakład Anatomii
dc.affiliationpl
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego
dc.affiliationpl
Wydział Lekarski : Zakład Biofizyki
dc.cm.id
65989
dc.contributor.authorpl
Orzechowska, Sylwia - 108746
dc.contributor.authorpl
Wróbel, Andrzej - 132782
dc.contributor.authorpl
Goncerz, Grzegorz - 129557
dc.contributor.authorpl
Podolec, Piotr - 133185
dc.contributor.authorpl
Rokita, Eugeniusz - 133291
dc.date.accessioned
2015-05-26T14:09:21Z
dc.date.available
2015-05-26T14:09:21Z
dc.date.issuedpl
2014
dc.description.adminpl
Rokita, Eugeniusz [SAP20001931] - druga afiliacja autora
dc.description.numberpl
1
dc.description.physicalpl
40-47
dc.description.publicationpl
0,5
dc.description.volumepl
23
dc.identifier.eissnpl
2053-2644
dc.identifier.issnpl
0966-8519
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/8157
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
Bez licencji otwartego dostępu
dc.rights.uri*
dc.subtypepl
Article
dc.titlepl
Physicochemical and micro-tomographic characterization of inorganic deposits associated with aortic stenosis
dc.title.journalpl
The Journal of Heart Valve Disease
dc.typepl
JournalArticle
dspace.entity.type
Publication
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