Bioreactor study employing bacteria with enhanced activity toward cyanobacterial toxins microcystins

2014
journal article
article
31
dc.abstract.enAn important aim of white (grey) biotechnology is bioremediation, where microbes are employed to remove unwanted chemicals. Microcystins (MCs) and other cyanobacterial toxins are not industrial or agricultural pollutants; however, their occurrence as a consequence of human activity and water reservoir eutrophication is regarded as anthropogenic. Microbial degradation of microcystins is suggested as an alternative to chemical and physical methods of their elimination. This paper describes a possible technique of the practical application of the biodegradation process. The idea relies on the utilization of bacteria with a significantly enhanced MC-degradation ability (in comparison with wild strains). The cells of an Escherichia coli laboratory strain expressing microcystinase (MlrA) responsible for the detoxification of MCs were immobilized in alginate beads. The degradation potency of the tested bioreactors was monitored by HPLC detection of linear microcystin LR (MC-LR) as the MlrA degradation product. An open system based on a column filled with alginate-entrapped cells was shown to operate more efficiently than a closed system (alginate beads shaken in a glass container). The maximal degradation rate calculated per one liter of carrier was 219.9 µg h−1 of degraded MC-LR. A comparison of the efficiency of the described system with other biological and chemo-physical proposals suggests that this new idea presents several advantages and is worth investigating in future studies.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Analitycznejpl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Fizjologii i Biologii Rozwoju Roślinpl
dc.contributor.authorDziga, Dariusz - 127864 pl
dc.contributor.authorLiszniańska, Magdalena - 163385 pl
dc.contributor.authorWładyka, Benedykt - 132671 pl
dc.date.accessioned2015-04-24T08:36:46Z
dc.date.available2015-04-24T08:36:46Z
dc.date.issued2014pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBibliogr. s. 2390-2392pl
dc.description.number8pl
dc.description.physical2379-2392pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume6pl
dc.identifier.doi10.3390/toxins6082379pl
dc.identifier.eissn2072-6651pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/5639
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Polska*
dc.rights.licenceCC-BY-NC-ND
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/pl/legalcode*
dc.share.typeotwarte czasopismo
dc.subject.enmicrocystinpl
dc.subject.enbioreactorpl
dc.subject.enmicrobial degradationpl
dc.subtypeArticlepl
dc.titleBioreactor study employing bacteria with enhanced activity toward cyanobacterial toxins microcystinspl
dc.title.journalToxinspl
dc.title.volumeHarmful algal blooms (HABs) and public health : progress and current challengespl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
An important aim of white (grey) biotechnology is bioremediation, where microbes are employed to remove unwanted chemicals. Microcystins (MCs) and other cyanobacterial toxins are not industrial or agricultural pollutants; however, their occurrence as a consequence of human activity and water reservoir eutrophication is regarded as anthropogenic. Microbial degradation of microcystins is suggested as an alternative to chemical and physical methods of their elimination. This paper describes a possible technique of the practical application of the biodegradation process. The idea relies on the utilization of bacteria with a significantly enhanced MC-degradation ability (in comparison with wild strains). The cells of an Escherichia coli laboratory strain expressing microcystinase (MlrA) responsible for the detoxification of MCs were immobilized in alginate beads. The degradation potency of the tested bioreactors was monitored by HPLC detection of linear microcystin LR (MC-LR) as the MlrA degradation product. An open system based on a column filled with alginate-entrapped cells was shown to operate more efficiently than a closed system (alginate beads shaken in a glass container). The maximal degradation rate calculated per one liter of carrier was 219.9 µg h−1 of degraded MC-LR. A comparison of the efficiency of the described system with other biological and chemo-physical proposals suggests that this new idea presents several advantages and is worth investigating in future studies.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Analitycznej
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Fizjologii i Biologii Rozwoju Roślin
dc.contributor.authorpl
Dziga, Dariusz - 127864
dc.contributor.authorpl
Liszniańska, Magdalena - 163385
dc.contributor.authorpl
Władyka, Benedykt - 132671
dc.date.accessioned
2015-04-24T08:36:46Z
dc.date.available
2015-04-24T08:36:46Z
dc.date.issuedpl
2014
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Bibliogr. s. 2390-2392
dc.description.numberpl
8
dc.description.physicalpl
2379-2392
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
6
dc.identifier.doipl
10.3390/toxins6082379
dc.identifier.eissnpl
2072-6651
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/5639
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 Polska
dc.rights.licence
CC-BY-NC-ND
dc.rights.uri*
http://creativecommons.org/licenses/by-nc-nd/3.0/pl/legalcode
dc.share.type
otwarte czasopismo
dc.subject.enpl
microcystin
dc.subject.enpl
bioreactor
dc.subject.enpl
microbial degradation
dc.subtypepl
Article
dc.titlepl
Bioreactor study employing bacteria with enhanced activity toward cyanobacterial toxins microcystins
dc.title.journalpl
Toxins
dc.title.volumepl
Harmful algal blooms (HABs) and public health : progress and current challenges
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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