Precise mass determination of single cell with cantilever-based microbiosensor system

Bibliographic description

punktacja MEiN [2017 A]: 40

title:	Precise mass determination of single cell with cantilever-based microbiosensor system
author:	Łabędź Bogdan , Wańczyk Aleksandra , Rajfur Zenon
journal title:	PLoS ONE
volume:	12
issue:	11
date of publication :	2017
article ID:	e0188388
eISSN:	1932-6203
DOI:	10.1371/journal.pone.0188388
language:	English
journal language:	English
abstract in English:	Having determined the mass of a single cell of brewer yeast Saccharomyces cerevisiae by means of a microcantilever-based biosensor Cantisens CSR-801 (Concentris, Basel, Switzerland), it was found that its dry mass is 47,65 $\pm$ 1,05 pg. Found to be crucial in this mass determination was the cell position along the length of the cantilever. Moreover, calculations including cells positions on the cantilever provide a threefold better degree of accuracy than those which assume uniform mass distribution. We have also examined the influence of storage time on the single cell mass. Our results show that after 6 months there is an increase in the average mass of a single yeast cell.
affiliation:	Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego
type:	journal article
subtype:	academic paper