Influence of macrophage activation on their capacity to bind bacterial antigens studied with atomic force microscopy

2006
journal article
article
11
cris.lastimport.wos2024-04-09T18:46:28Z
dc.abstract.enIn this work we studied interactions between bacterial antigens and receptors on the surface of macrophages using atomic force microscopy (AFM). We used two bacterial cell wall components: lipopolysaccharide (LPS) derived from gram-negative Escherichia coli and exopolysaccharide (EPS) derived from gram-positive Lactobacillus rhamnosus. Interactions between these bacterial antigens and immune cell receptors were studied in peritoneal macrophages derived from two strains of mice, CBA and C3H/J, in which the Toll-like receptor 4 (TLR4) is genetically disabled. We collected 500 force-distance curves for LPS-activated cells using an EPS-covered AFM tip, and for EPS-activated cells using an LPS-covered AFM tip. Nonactivated cells were tested as reference cells. The results show that LPS-primed macrophages decrease their ability to bind EPS. Surprisingly, EPS-activated macrophages maintain or even increase their ability to bind LPS. This may suggest that in vivo commensal enteric bacteria, such as lactobacilli, will enhance the defense potential of local macrophages against pathogens expressing LPS.pl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiegopl
dc.affiliationWydział Lekarski : Zakład Immunologiipl
dc.affiliationWydział Lekarski : Zakład Bakteriologii,Ekologii Drobnoustrojów i Parazytologiipl
dc.contributor.authorTargosz, Marta - 102459 pl
dc.contributor.authorLabuda, Aleksanderpl
dc.contributor.authorCzuba, Paweł - 100010 pl
dc.contributor.authorBiedroń, Rafał - 128746 pl
dc.contributor.authorStrus, Magdalena - 133523 pl
dc.contributor.authorGamian, Andrzejpl
dc.contributor.authorMarcinkiewicz, Janusz - 130850 pl
dc.contributor.authorSzymoński, Marek - 132296 pl
dc.date.accessioned2017-01-03T14:08:20Z
dc.date.available2017-01-03T14:08:20Z
dc.date.issued2006pl
dc.description.number2pl
dc.description.physical82-88pl
dc.description.volume2pl
dc.identifier.doi10.1016/j.nano.2006.04.006pl
dc.identifier.eissn1549-9642pl
dc.identifier.issn1549-9634pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/34839
dc.languageengpl
dc.language.containerengpl
dc.rightsDodaję tylko opis bibliograficzny*
dc.rights.licenceBez licencji otwartego dostępu
dc.rights.uri*
dc.subject.enatomic force microscopy (AFM)pl
dc.subject.enexopolysaccharide (EPS)pl
dc.subject.enforce spectroscopypl
dc.subject.enlipopolysaccharide (LPS)pl
dc.subject.enmacrophagespl
dc.subtypeArticlepl
dc.titleInfluence of macrophage activation on their capacity to bind bacterial antigens studied with atomic force microscopypl
dc.title.journalNanomedicine. Nanotechnology, Biology and Medicinepl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T18:46:28Z
dc.abstract.enpl
In this work we studied interactions between bacterial antigens and receptors on the surface of macrophages using atomic force microscopy (AFM). We used two bacterial cell wall components: lipopolysaccharide (LPS) derived from gram-negative Escherichia coli and exopolysaccharide (EPS) derived from gram-positive Lactobacillus rhamnosus. Interactions between these bacterial antigens and immune cell receptors were studied in peritoneal macrophages derived from two strains of mice, CBA and C3H/J, in which the Toll-like receptor 4 (TLR4) is genetically disabled. We collected 500 force-distance curves for LPS-activated cells using an EPS-covered AFM tip, and for EPS-activated cells using an LPS-covered AFM tip. Nonactivated cells were tested as reference cells. The results show that LPS-primed macrophages decrease their ability to bind EPS. Surprisingly, EPS-activated macrophages maintain or even increase their ability to bind LPS. This may suggest that in vivo commensal enteric bacteria, such as lactobacilli, will enhance the defense potential of local macrophages against pathogens expressing LPS.
dc.affiliationpl
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego
dc.affiliationpl
Wydział Lekarski : Zakład Immunologii
dc.affiliationpl
Wydział Lekarski : Zakład Bakteriologii,Ekologii Drobnoustrojów i Parazytologii
dc.contributor.authorpl
Targosz, Marta - 102459
dc.contributor.authorpl
Labuda, Aleksander
dc.contributor.authorpl
Czuba, Paweł - 100010
dc.contributor.authorpl
Biedroń, Rafał - 128746
dc.contributor.authorpl
Strus, Magdalena - 133523
dc.contributor.authorpl
Gamian, Andrzej
dc.contributor.authorpl
Marcinkiewicz, Janusz - 130850
dc.contributor.authorpl
Szymoński, Marek - 132296
dc.date.accessioned
2017-01-03T14:08:20Z
dc.date.available
2017-01-03T14:08:20Z
dc.date.issuedpl
2006
dc.description.numberpl
2
dc.description.physicalpl
82-88
dc.description.volumepl
2
dc.identifier.doipl
10.1016/j.nano.2006.04.006
dc.identifier.eissnpl
1549-9642
dc.identifier.issnpl
1549-9634
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/34839
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Dodaję tylko opis bibliograficzny
dc.rights.licence
Bez licencji otwartego dostępu
dc.rights.uri*
dc.subject.enpl
atomic force microscopy (AFM)
dc.subject.enpl
exopolysaccharide (EPS)
dc.subject.enpl
force spectroscopy
dc.subject.enpl
lipopolysaccharide (LPS)
dc.subject.enpl
macrophages
dc.subtypepl
Article
dc.titlepl
Influence of macrophage activation on their capacity to bind bacterial antigens studied with atomic force microscopy
dc.title.journalpl
Nanomedicine. Nanotechnology, Biology and Medicine
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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