Strain specific immunostimulatory potential of lactobacilli-derived exopolysaccharides

2011
journal article
article
dc.abstract.enLactobacilli, the most commonly used probiotics, show strain specific immunostimulatory effects. The strain specificity is related to the structure of cell-wall components such as lipoteichoic acids (LTAs), ligands for TLR2. In contrast, the role of exopolysaccharides (EPSs), the major components of lactobacilli biofilm, in the “cross-talk” between bacteria and the immune system is poorly documented. In this study, we have tested immunomodulating potential of lactobacilli derived EPSs, by their ability to modulate in vitro production of cytokines by murine peritoneal macrophages. We have examined EPSs of three lactobacilli strains (L. reuteri, L. johnsonii, L. animalis/murinus) isolated from colitic mice. The effect was compared with that of whole bacteria and LTA. All three nonviable Lactobacillus strains induced high amounts of cytokines with a strain specific profile. In contrast, the tested exopolysaccharides, exert low, albeit highly strain diversified stimulatory activities. The immunostimulatory potential of EPSs was correlated with the interaction strength between EPS and macrophage receptors as measured by atomic force spectroscopy.pl
dc.affiliationWydział Lekarski : Zakład Immunologiipl
dc.affiliationWydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiegopl
dc.affiliationWydział Lekarski : Zakład Bakteriologii,Ekologii Drobnoustrojów i Parazytologiipl
dc.contributor.authorCiszek-Lenda, Marta - 129072 pl
dc.contributor.authorStrus, Magdalena - 133523 pl
dc.contributor.authorGórska-Frączek, Sabinapl
dc.contributor.authorTargosz-Korecka, Marta - 102459 pl
dc.contributor.authorŚróttek, Małgorzata - 128792 pl
dc.contributor.authorHeczko, Piotr - 129700 pl
dc.contributor.authorGamian, Andrzejpl
dc.contributor.authorSzymoński, Marek - 132296 pl
dc.contributor.authorMarcinkiewicz, Janusz - 130850 pl
dc.date.accession2017-01-03pl
dc.date.accessioned2017-01-03T13:51:06Z
dc.date.available2017-01-03T13:51:06Z
dc.date.issued2011pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number3pl
dc.description.physical121-129pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume36pl
dc.identifier.eissn1644-4124pl
dc.identifier.issn1426-3912pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/34835
dc.identifier.weblinkhttp://www.termedia.pl/-Experimental-immunology-Strain-specific-immunostimulatory-potential-of-lactobacilli-derived-exopolysaccharides,10,17497,1,1.htmlpl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Na tych samych warunkach 4.0 Międzynarodowa*
dc.rights.licenceCC-BY-NC-SA
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.encytokinespl
dc.subject.enmacrophagespl
dc.subject.enlipoteichoic acid (LTA)pl
dc.subject.enexopolysaccharide (EPS)pl
dc.subject.enprobioticspl
dc.subject.enlactobacillipl
dc.subtypeArticlepl
dc.titleStrain specific immunostimulatory potential of lactobacilli-derived exopolysaccharidespl
dc.title.journalCentral-European Journal of Immunologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Lactobacilli, the most commonly used probiotics, show strain specific immunostimulatory effects. The strain specificity is related to the structure of cell-wall components such as lipoteichoic acids (LTAs), ligands for TLR2. In contrast, the role of exopolysaccharides (EPSs), the major components of lactobacilli biofilm, in the “cross-talk” between bacteria and the immune system is poorly documented. In this study, we have tested immunomodulating potential of lactobacilli derived EPSs, by their ability to modulate in vitro production of cytokines by murine peritoneal macrophages. We have examined EPSs of three lactobacilli strains (L. reuteri, L. johnsonii, L. animalis/murinus) isolated from colitic mice. The effect was compared with that of whole bacteria and LTA. All three nonviable Lactobacillus strains induced high amounts of cytokines with a strain specific profile. In contrast, the tested exopolysaccharides, exert low, albeit highly strain diversified stimulatory activities. The immunostimulatory potential of EPSs was correlated with the interaction strength between EPS and macrophage receptors as measured by atomic force spectroscopy.
dc.affiliationpl
Wydział Lekarski : Zakład Immunologii
dc.affiliationpl
Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego
dc.affiliationpl
Wydział Lekarski : Zakład Bakteriologii,Ekologii Drobnoustrojów i Parazytologii
dc.contributor.authorpl
Ciszek-Lenda, Marta - 129072
dc.contributor.authorpl
Strus, Magdalena - 133523
dc.contributor.authorpl
Górska-Frączek, Sabina
dc.contributor.authorpl
Targosz-Korecka, Marta - 102459
dc.contributor.authorpl
Śróttek, Małgorzata - 128792
dc.contributor.authorpl
Heczko, Piotr - 129700
dc.contributor.authorpl
Gamian, Andrzej
dc.contributor.authorpl
Szymoński, Marek - 132296
dc.contributor.authorpl
Marcinkiewicz, Janusz - 130850
dc.date.accessionpl
2017-01-03
dc.date.accessioned
2017-01-03T13:51:06Z
dc.date.available
2017-01-03T13:51:06Z
dc.date.issuedpl
2011
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
3
dc.description.physicalpl
121-129
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
36
dc.identifier.eissnpl
1644-4124
dc.identifier.issnpl
1426-3912
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/34835
dc.identifier.weblinkpl
http://www.termedia.pl/-Experimental-immunology-Strain-specific-immunostimulatory-potential-of-lactobacilli-derived-exopolysaccharides,10,17497,1,1.html
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Na tych samych warunkach 4.0 Międzynarodowa
dc.rights.licence
CC-BY-NC-SA
dc.rights.uri*
http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
cytokines
dc.subject.enpl
macrophages
dc.subject.enpl
lipoteichoic acid (LTA)
dc.subject.enpl
exopolysaccharide (EPS)
dc.subject.enpl
probiotics
dc.subject.enpl
lactobacilli
dc.subtypepl
Article
dc.titlepl
Strain specific immunostimulatory potential of lactobacilli-derived exopolysaccharides
dc.title.journalpl
Central-European Journal of Immunology
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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