Towards the understanding of the behavior of single-chained ether phospholipids in model biomembranes : interactions with phosphatidylethanolamines in Langmuir monolayers
Towards the understanding of the behavior of single-chained ether phospholipids in model biomembranes : interactions with phosphatidylethanolamines in Langmuir monolayers
Towards the understanding of the behavior of single-chained ether phospholipids in model biomembranes : interactions with phosphatidylethanolamines in Langmuir monolayers
author:
Hąc-Wydro Katarzyna , Flasiński Michał , Wydro Paweł , Dynarowicz-Łątka Patrycja
Three
single-chained
ether
lipids
of
comparable
chemical
structure
but
different
biological
activities
(namely
natural
platelet
activating
factor
– PAF,
its
deacetylated
precursor
– lyso-PAF
and
synthetic
compound
–
edelfosine
–
ED)
have
been
investigated
in
mixed
Langmuir
monolayers
with
phos-
phatidylethanolamines,
PEs
(DSPE,
SOPE
and
DOPE),
serving
as
model
of
inner
shell
of
cellular
membrane.
The
aim
of
undertaken
studies
was
to
verify
the
correlation
between
minor
differences
in
chemical
struc-
tures
of
the
investigated
ether
lipids
and
their
behavior
in
membrane-mimicking
environment.
To
reach
this
goal
the
interactions
between
particular
ether
lipids
and
PEs
have
been
analyzed
with
G
Exc
values
derived
from
the
surface
pressure–area
isotherms.
To
get
insight
into
miscibility
between
film
com-
ponents,
Brewster
angle
microscopy,
enabling
direct
visualization
of
monolayers
structure,
has
been
applied.
The
obtained
results
prove
significant
differences
in
both
mixing
properties
and
the
interactions
in
the
investigated
systems.
On
one
hand,
they
are
related
to
the
structure
of
polar
head-groups
of
the
studied
ether
lipids,
which
determine
their
capability
of
hydrogen
bond(s)
formation
with
head-groups
of
PEs.
Edelfosine,
lacking
this
property,
interacts
with
PEs
the
most
unfavorably
among
all
the
inves-
tigated
compounds.
Another
important
parameter
in
this
context
is
the
structure
of
PEs
monolayers
–
the
most
closely
packed
DSPE
film
was
found
to
be
most
unfavorable
for
incorporation
of
ether
lipid
molecules.
Our
results
prove
that
the
analysis
of
the
interaction
between
ether
lipids
and
components
of
biomembrane
in
Langmuir
monolayers
is
a
potent
method
to
explain
differences
in
biological
activity
of
the
investigated
ether
lipids.