Intralesional injection of interferon gamma affects reactive proliferation of astrocytes in the neonatal rat brain : a preliminary study of the age-dependent effect

1999
journal article
article
dc.abstract.enFollowing a mechanical lesion of the left cerebral hemisphere, newborn male rats received a single injection of recombinant rat interferon gamma (IFN gamma) into the lesion cavity at doses of 5, 50 and 500 U. One or two days after the injury the rats were injected with H-3-thymidine. Brain sections were immunostained for glial fibrillary acidic protein (GFAP), subjected to autoradiography and examined microscopically to record proliferating GFAP-immunopositive (GFAP+) astrocytes labeled with H-3-thymidine. Following the intermediate 50 U dose of IFN gamma, numbers of GFAP+ astrocytes and of their mitoses on day 1 after injury were significantly higher than in controls. Nevertheless, the astrocyte labeling index remained at the control level. Injections of the minimal 5 U or the maximal 500 U doses of IFN gamma had no effect on that day. On day 2, however, each of the three doses evoked a statistically significant but dose-independent reduction of the labeling index without similar changes in total number of GFAP+ astrocytes or in numbers of their divisions. The changes appear to indicate a non-linear relation between the intensity of reactive behaviour of astrocytes and the amount of IFN gamma injected into the lesion area. On the basis of previous publications, IFN gamma effects on the astrocyte reactivity are discussed as being age-dependent.pl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Zoologiipl
dc.contributor.authorPawliński, Rafałpl
dc.contributor.authorSetkowicz-Janeczko, Zuzanna - 131836 pl
dc.contributor.authorZiaja, Marek - 132904 pl
dc.contributor.authorSołtys, Zbigniew - 147734 pl
dc.contributor.authorJaneczko, Krzysztof - 128444 pl
dc.date.accessioned2016-10-28T10:42:18Z
dc.date.available2016-10-28T10:42:18Z
dc.date.issued1999pl
dc.description.additionalZuzanna Setkowicz-Janeczko podpisana nazwiskiem Setkowiczpl
dc.description.number4pl
dc.description.physical237-242pl
dc.description.volume37pl
dc.identifier.eissn1897-5631pl
dc.identifier.issn0239-8508pl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/31865
dc.languageengpl
dc.language.containerengpl
dc.rights.licenceBez licencji otwartego dostępu
dc.subtypeArticlepl
dc.titleIntralesional injection of interferon gamma affects reactive proliferation of astrocytes in the neonatal rat brain : a preliminary study of the age-dependent effectpl
dc.title.journalFolia Histochemica et Cytobiologicapl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Following a mechanical lesion of the left cerebral hemisphere, newborn male rats received a single injection of recombinant rat interferon gamma (IFN gamma) into the lesion cavity at doses of 5, 50 and 500 U. One or two days after the injury the rats were injected with H-3-thymidine. Brain sections were immunostained for glial fibrillary acidic protein (GFAP), subjected to autoradiography and examined microscopically to record proliferating GFAP-immunopositive (GFAP+) astrocytes labeled with H-3-thymidine. Following the intermediate 50 U dose of IFN gamma, numbers of GFAP+ astrocytes and of their mitoses on day 1 after injury were significantly higher than in controls. Nevertheless, the astrocyte labeling index remained at the control level. Injections of the minimal 5 U or the maximal 500 U doses of IFN gamma had no effect on that day. On day 2, however, each of the three doses evoked a statistically significant but dose-independent reduction of the labeling index without similar changes in total number of GFAP+ astrocytes or in numbers of their divisions. The changes appear to indicate a non-linear relation between the intensity of reactive behaviour of astrocytes and the amount of IFN gamma injected into the lesion area. On the basis of previous publications, IFN gamma effects on the astrocyte reactivity are discussed as being age-dependent.
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
dc.contributor.authorpl
Pawliński, Rafał
dc.contributor.authorpl
Setkowicz-Janeczko, Zuzanna - 131836
dc.contributor.authorpl
Ziaja, Marek - 132904
dc.contributor.authorpl
Sołtys, Zbigniew - 147734
dc.contributor.authorpl
Janeczko, Krzysztof - 128444
dc.date.accessioned
2016-10-28T10:42:18Z
dc.date.available
2016-10-28T10:42:18Z
dc.date.issuedpl
1999
dc.description.additionalpl
Zuzanna Setkowicz-Janeczko podpisana nazwiskiem Setkowicz
dc.description.numberpl
4
dc.description.physicalpl
237-242
dc.description.volumepl
37
dc.identifier.eissnpl
1897-5631
dc.identifier.issnpl
0239-8508
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/31865
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights.licence
Bez licencji otwartego dostępu
dc.subtypepl
Article
dc.titlepl
Intralesional injection of interferon gamma affects reactive proliferation of astrocytes in the neonatal rat brain : a preliminary study of the age-dependent effect
dc.title.journalpl
Folia Histochemica et Cytobiologica
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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