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Interaction of selected divalent metal ions with human ataxin-3 Q36
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Interaction of selected divalent metal ions with human ataxin-3 Q36
Bibliographic description
| title: | Interaction of selected divalent metal ions with human ataxin-3 Q36 |
| author: | Stawoska Iwona, Wesełucha-Birczyńska Aleksandra  , Regonesi Maria Elena, Riva Matteo, Tortora Paolo, Stochel Grażyna
|
| journal title: | JBIC. Journal of Biological Inorganic Chemistry |
| volume: | 14 |
| issue: | 8 |
| date of publication : | 2009 |
| pages: | 1175-1185 |
| ISSN: |
0949-8257 |
| eISSN: |
1432-1327 |
| DOI: | |
| language: | English |
| journal language: | English |
| abstract in English: | The mode of interaction of ataxin-3 Q36 (AT-3 Q36) with selected endogenous and exogenous metal ions, namely, $Zn^{2+}$, $Cu^{2+}$, $Ni^{2+}$, and $Cd^{2+}$, was examined. Metal-ion-induced structural changes of the protein were monitored by fluorescence as well as Fourier transform Raman spectroscopy. We found that the cations tested lead to a decrease in $\alpha$-helical content and a concurrent increase in $\beta$-sheet as well as undefined ($\beta$-turn and random-coil) structures. The most evident effect was observed for copper and nickel cations. After titration with these cations, the AT3 Q36 secondary structure content (27% $\alpha$-helices in the presence of either ion, 31 and 27% $\beta$-sheets for $Cu^{2+}$ and $Ni^{2+}$, respectively) was similar to that observed for the aggregated form of the protein (27% $\alpha$-helices, 36% $\beta$-sheets). Using the 1-anilinonaphthalene-8-sulfonate hydrophobic fluorescence probe, we showed that the presence of the metal ions tested led to the formation of solvent-exposed hydrophobic patches of AT-3 Q36, and that such an effect decreased with increasing ionic radius. |
| keywords in English: | ataxin-3, Fourier transform Raman, metal ions, secondary structures |
| affiliation: | Wydział Chemii : Zakład Chemii Nieorganicznej, Wydział Chemii : Zakład Fizyki Chemicznej |
| type: | journal article |
| subtype: | academic paper |
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Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
