Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

2022
journal article
article
19
cris.lastimport.wos2024-04-09T21:08:43Z
dc.abstract.enImplant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment of biofilm-related infections. Here, we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. The mAbs were also shown to bind a collection of clinical isolates derived from different biofilm-associated infections (endocarditis, prosthetic joint, catheter). We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. Furthermore, we show that a mAb recognizing wall teichoic acid (clone 4497) specifically localizes to a subcutaneously implanted pre-colonized catheter in mice. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo.pl
dc.contributor.authorde Vor, Lisannepl
dc.contributor.authorvan Dijk, Brucepl
dc.contributor.authorvan Kessel, Kokpl
dc.contributor.authorKavanaugh, Jeffrey S.pl
dc.contributor.authorde Haas, Carlapl
dc.contributor.authorAerts, Piet C.pl
dc.contributor.authorViveen, Marco C.pl
dc.contributor.authorBoel, Edwin C.pl
dc.contributor.authorFluit, Ad C.pl
dc.contributor.authorKwieciński, Jakub - 103976 pl
dc.contributor.authorKrijger, Gerard C.pl
dc.contributor.authorRamakers, Ruud M.pl
dc.contributor.authorBeekman, Freek J.pl
dc.contributor.authorDadachova, Ekaterinapl
dc.contributor.authorLam, Marnix G. E. H.pl
dc.contributor.authorVogely, H. Charlespl
dc.contributor.authorvan der Wal, Bart C. H.pl
dc.contributor.authorvan Strijp, Jos A. G.pl
dc.contributor.authorHorswill, Alexander R.pl
dc.contributor.authorWeinans, Harriepl
dc.contributor.authorRooijakkers, Suzan H. M.pl
dc.date.accessioned2023-06-16T06:45:39Z
dc.date.available2023-06-16T06:45:39Z
dc.date.issued2022pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalJakub Kwieciński podpisany: Jakub M. Kwieciński. Bibliogr.pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume11pl
dc.identifier.articleide67301pl
dc.identifier.doi10.7554/eLife.67301pl
dc.identifier.eissn2050-084Xpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/312334
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enStaphylococcus aureuspl
dc.subject.enbiofilmpl
dc.subject.enmonoclonal antibodiespl
dc.subtypeArticlepl
dc.titleHuman monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilmpl
dc.title.journaleLifepl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T21:08:43Z
dc.abstract.enpl
Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment of biofilm-related infections. Here, we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. The mAbs were also shown to bind a collection of clinical isolates derived from different biofilm-associated infections (endocarditis, prosthetic joint, catheter). We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. Furthermore, we show that a mAb recognizing wall teichoic acid (clone 4497) specifically localizes to a subcutaneously implanted pre-colonized catheter in mice. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo.
dc.contributor.authorpl
de Vor, Lisanne
dc.contributor.authorpl
van Dijk, Bruce
dc.contributor.authorpl
van Kessel, Kok
dc.contributor.authorpl
Kavanaugh, Jeffrey S.
dc.contributor.authorpl
de Haas, Carla
dc.contributor.authorpl
Aerts, Piet C.
dc.contributor.authorpl
Viveen, Marco C.
dc.contributor.authorpl
Boel, Edwin C.
dc.contributor.authorpl
Fluit, Ad C.
dc.contributor.authorpl
Kwieciński, Jakub - 103976
dc.contributor.authorpl
Krijger, Gerard C.
dc.contributor.authorpl
Ramakers, Ruud M.
dc.contributor.authorpl
Beekman, Freek J.
dc.contributor.authorpl
Dadachova, Ekaterina
dc.contributor.authorpl
Lam, Marnix G. E. H.
dc.contributor.authorpl
Vogely, H. Charles
dc.contributor.authorpl
van der Wal, Bart C. H.
dc.contributor.authorpl
van Strijp, Jos A. G.
dc.contributor.authorpl
Horswill, Alexander R.
dc.contributor.authorpl
Weinans, Harrie
dc.contributor.authorpl
Rooijakkers, Suzan H. M.
dc.date.accessioned
2023-06-16T06:45:39Z
dc.date.available
2023-06-16T06:45:39Z
dc.date.issuedpl
2022
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Jakub Kwieciński podpisany: Jakub M. Kwieciński. Bibliogr.
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
11
dc.identifier.articleidpl
e67301
dc.identifier.doipl
10.7554/eLife.67301
dc.identifier.eissnpl
2050-084X
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/312334
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
Staphylococcus aureus
dc.subject.enpl
biofilm
dc.subject.enpl
monoclonal antibodies
dc.subtypepl
Article
dc.titlepl
Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm
dc.title.journalpl
eLife
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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