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Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm
Staphylococcus aureus
biofilm
monoclonal antibodies
Jakub Kwieciński podpisany: Jakub M. Kwieciński. Bibliogr.
Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment of biofilm-related infections. Here, we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. The mAbs were also shown to bind a collection of clinical isolates derived from different biofilm-associated infections (endocarditis, prosthetic joint, catheter). We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. Furthermore, we show that a mAb recognizing wall teichoic acid (clone 4497) specifically localizes to a subcutaneously implanted pre-colonized catheter in mice. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo.
cris.lastimport.wos | 2024-04-09T21:08:43Z | |
dc.abstract.en | Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment of biofilm-related infections. Here, we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. The mAbs were also shown to bind a collection of clinical isolates derived from different biofilm-associated infections (endocarditis, prosthetic joint, catheter). We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. Furthermore, we show that a mAb recognizing wall teichoic acid (clone 4497) specifically localizes to a subcutaneously implanted pre-colonized catheter in mice. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo. | pl |
dc.contributor.author | de Vor, Lisanne | pl |
dc.contributor.author | van Dijk, Bruce | pl |
dc.contributor.author | van Kessel, Kok | pl |
dc.contributor.author | Kavanaugh, Jeffrey S. | pl |
dc.contributor.author | de Haas, Carla | pl |
dc.contributor.author | Aerts, Piet C. | pl |
dc.contributor.author | Viveen, Marco C. | pl |
dc.contributor.author | Boel, Edwin C. | pl |
dc.contributor.author | Fluit, Ad C. | pl |
dc.contributor.author | Kwieciński, Jakub - 103976 | pl |
dc.contributor.author | Krijger, Gerard C. | pl |
dc.contributor.author | Ramakers, Ruud M. | pl |
dc.contributor.author | Beekman, Freek J. | pl |
dc.contributor.author | Dadachova, Ekaterina | pl |
dc.contributor.author | Lam, Marnix G. E. H. | pl |
dc.contributor.author | Vogely, H. Charles | pl |
dc.contributor.author | van der Wal, Bart C. H. | pl |
dc.contributor.author | van Strijp, Jos A. G. | pl |
dc.contributor.author | Horswill, Alexander R. | pl |
dc.contributor.author | Weinans, Harrie | pl |
dc.contributor.author | Rooijakkers, Suzan H. M. | pl |
dc.date.accessioned | 2023-06-16T06:45:39Z | |
dc.date.available | 2023-06-16T06:45:39Z | |
dc.date.issued | 2022 | pl |
dc.date.openaccess | 0 | |
dc.description.accesstime | w momencie opublikowania | |
dc.description.additional | Jakub Kwieciński podpisany: Jakub M. Kwieciński. Bibliogr. | pl |
dc.description.version | ostateczna wersja wydawcy | |
dc.description.volume | 11 | pl |
dc.identifier.articleid | e67301 | pl |
dc.identifier.doi | 10.7554/eLife.67301 | pl |
dc.identifier.eissn | 2050-084X | pl |
dc.identifier.uri | https://ruj.uj.edu.pl/xmlui/handle/item/312334 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa | * |
dc.rights.licence | CC-BY | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/legalcode.pl | * |
dc.share.type | otwarte czasopismo | |
dc.subject.en | Staphylococcus aureus | pl |
dc.subject.en | biofilm | pl |
dc.subject.en | monoclonal antibodies | pl |
dc.subtype | Article | pl |
dc.title | Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm | pl |
dc.title.journal | eLife | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |