Biological properties of 12 newly isolated Acinetobacter baumannii-specific bacteriophages

2023
journal article
article
12
cris.lastimport.wos2024-04-09T23:24:33Z
dc.abstract.enInfections with the opportunistic Gram-negative bacterium Acinetobacter baumannii pose a serious threat today, which is aggravated by the growing problem of multi-drug resistance among bacteria, caused by the overuse of antibiotics. Treatment of infections caused by antibiotic-resistant A. baumannii strains with the use of phage therapy is not only a promising alternative, but sometimes the only option. Therefore, phages specific for clinical multi-drug resistant A. baumannii were searched for in environmental, municipal, and hospital wastewater samples collected from different locations in Poland. The conducted research allowed us to determine the biological properties and morphology of the tested phages. As a result of our research, 12 phages specific for A. baumannii, 11 of which turned out to be temperate and only one lytic, were isolated. Their lytic spectra ranged from 11 to 75%. The plaques formed by most phages were small and transparent, while one of them formed relatively large plaques with a clearly marked ‘halo’ effect. Based on Transmission Electron Microscopy (TEM), most of our phages have been classified as siphoviruses (only one phage was classified as a podovirus). All phages have icosahedral capsid symmetry, and 11 of them have a long tail. Optimal multiplicity of infections (MOIs) and the adsorption rate were also determined. MOI values varied depending on the phage—from 0.001 to 10. Based on similarities to known bacteriophages, our A. baumannii-specific phages have been proposed to belong to the Beijerinckvirinae and Junivirinae subfamilies. This study provides an additional tool in the fight against this important pathogen and may boost the interest in phage therapy as an alternative and supplement to the current antibiotics
dc.cm.date2023-02-27T21:31:39Z
dc.cm.id111248pl
dc.cm.idOmegaUJCM8cbc128bee574addad1446560e0113ecpl
dc.contributor.authorBagińska, Nataliapl
dc.contributor.authorHarhala, Marek Adampl
dc.contributor.authorCieślik, Martyna - 262624 pl
dc.contributor.authorOrwat, Filippl
dc.contributor.authorWeber-Dąbrowska, Beatapl
dc.contributor.authorDąbrowska, Krystynapl
dc.contributor.authorGórski, Andrzejpl
dc.contributor.authorJończyk-Matysiak, Ewapl
dc.date.accession2023-02-24pl
dc.date.accessioned2023-02-27T21:31:39Z
dc.date.available2023-02-27T21:31:39Z
dc.date.issued2023pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number1pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume15pl
dc.identifier.articleid231pl
dc.identifier.doi10.3390/v15010231pl
dc.identifier.eissn1999-4915pl
dc.identifier.issn1999-4915pl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/308395
dc.identifier.weblinkhttps://www.mdpi.com/1999-4915/15/1/231pl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.typeOtwarte czasopismo
dc.subject.enAcinetobacter baumannii
dc.subject.enantimicrobial resistance
dc.subject.enESKAPE
dc.subject.engram-negative bacteria
dc.subject.enphages
dc.subtypeArticlepl
dc.titleBiological properties of 12 newly isolated <I>Acinetobacter baumannii</I>-specific bacteriophagespl
dc.title.journalVirusespl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T23:24:33Z
dc.abstract.en
Infections with the opportunistic Gram-negative bacterium Acinetobacter baumannii pose a serious threat today, which is aggravated by the growing problem of multi-drug resistance among bacteria, caused by the overuse of antibiotics. Treatment of infections caused by antibiotic-resistant A. baumannii strains with the use of phage therapy is not only a promising alternative, but sometimes the only option. Therefore, phages specific for clinical multi-drug resistant A. baumannii were searched for in environmental, municipal, and hospital wastewater samples collected from different locations in Poland. The conducted research allowed us to determine the biological properties and morphology of the tested phages. As a result of our research, 12 phages specific for A. baumannii, 11 of which turned out to be temperate and only one lytic, were isolated. Their lytic spectra ranged from 11 to 75%. The plaques formed by most phages were small and transparent, while one of them formed relatively large plaques with a clearly marked ‘halo’ effect. Based on Transmission Electron Microscopy (TEM), most of our phages have been classified as siphoviruses (only one phage was classified as a podovirus). All phages have icosahedral capsid symmetry, and 11 of them have a long tail. Optimal multiplicity of infections (MOIs) and the adsorption rate were also determined. MOI values varied depending on the phage—from 0.001 to 10. Based on similarities to known bacteriophages, our A. baumannii-specific phages have been proposed to belong to the Beijerinckvirinae and Junivirinae subfamilies. This study provides an additional tool in the fight against this important pathogen and may boost the interest in phage therapy as an alternative and supplement to the current antibiotics
dc.cm.date
2023-02-27T21:31:39Z
dc.cm.idpl
111248
dc.cm.idOmegapl
UJCM8cbc128bee574addad1446560e0113ec
dc.contributor.authorpl
Bagińska, Natalia
dc.contributor.authorpl
Harhala, Marek Adam
dc.contributor.authorpl
Cieślik, Martyna - 262624
dc.contributor.authorpl
Orwat, Filip
dc.contributor.authorpl
Weber-Dąbrowska, Beata
dc.contributor.authorpl
Dąbrowska, Krystyna
dc.contributor.authorpl
Górski, Andrzej
dc.contributor.authorpl
Jończyk-Matysiak, Ewa
dc.date.accessionpl
2023-02-24
dc.date.accessioned
2023-02-27T21:31:39Z
dc.date.available
2023-02-27T21:31:39Z
dc.date.issuedpl
2023
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
1
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
15
dc.identifier.articleidpl
231
dc.identifier.doipl
10.3390/v15010231
dc.identifier.eissnpl
1999-4915
dc.identifier.issnpl
1999-4915
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/308395
dc.identifier.weblinkpl
https://www.mdpi.com/1999-4915/15/1/231
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
Otwarte czasopismo
dc.subject.en
Acinetobacter baumannii
dc.subject.en
antimicrobial resistance
dc.subject.en
ESKAPE
dc.subject.en
gram-negative bacteria
dc.subject.en
phages
dc.subtypepl
Article
dc.titlepl
Biological properties of 12 newly isolated <I>Acinetobacter baumannii</I>-specific bacteriophages
dc.title.journalpl
Viruses
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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