Proteomic profiling of ectosomes derived from paired urothelial bladder cancer and normal cells reveals the presence of biologically-relevant molecules

doi:10.3390/ijms22136816

Proteomic profiling of ectosomes derived from paired urothelial bladder cancer and normal cells reveals the presence of biologically-relevant molecules

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Proteomic profiling of ectosomes derived from paired urothelial bladder cancer and normal cells reveals the presence of biologically-relevant molecules

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dc.contributor.author	Surman, Magdalena [SAP14016164]	pl
dc.contributor.author	Kędracka-Krok, Sylwia [SAP11016010]	pl
dc.contributor.author	Jankowska, Urszula [SAP13905509]	pl
dc.contributor.author	Drożdż, Anna [USOS96343]	pl
dc.contributor.author	Stępień, Ewa [SAP14015168]	pl
dc.contributor.author	Przybyło, Małgorzata [SAP11116102]	pl
dc.date.accessioned	2021-07-12T16:53:45Z
dc.date.available	2021-07-12T16:53:45Z
dc.date.issued	2021	pl
dc.identifier.issn	1661-6596	pl
dc.identifier.uri	https://ruj.uj.edu.pl/xmlui/handle/item/276207
dc.language	eng	pl
dc.rights	Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/pl/legalcode	*
dc.title	Proteomic profiling of ectosomes derived from paired urothelial bladder cancer and normal cells reveals the presence of biologically-relevant molecules	pl
dc.type	JournalArticle	pl
dc.abstract.en	Protein content of extracellular vesicles (EVs) can modulate different processes during carcinogenesis. Novel proteomic strategies have been applied several times to profile proteins present in exosomes released by urothelial bladder cancer (UBC) cells. However, similar studies have not been conducted so far on another population of EVs, i.e., ectosomes. In the present study we used a shotgun nanoLC–MS/MS proteomic approach to investigate the protein content of ectosomes released in vitro by T-24 UBC cells and HCV-29 normal ureter epithelial cells. In addition, cancer-promoting effects exerted by UBC-derived ectosomes on non-invasive cells in terms of cell proliferation and migratory properties were assessed. In total, 1158 proteins were identified in T-24-derived ectosomes, while HCV-29-derived ectosomes contained a lower number of 259 identified proteins. Qualitative analysis revealed 938 proteins present uniquely in T-24-derived ectosomes, suggesting their potential applications in bladder cancer management as diagnostic and prognostic biomarkers. In addition, T-24-derived ectosomes increased proliferation and motility of recipient cells, likely due to the ectosomal transfer of the identified cancer-promoting molecules. The present study provided a focused identification of biologically relevant proteins in UBC-derived ectosomes, confirming their role in UBC development and progression, and their applicability for further biomarker-oriented studies in preclinical or clinical settings.	pl
dc.subject.en	biomarkers	pl
dc.subject.en	bladder cancer	pl
dc.subject.en	ectosomes	pl
dc.subject.en	extracellular vesicles	pl
dc.subject.en	nanoLC-MS/MS	pl
dc.subject.en	mass spectrometry	pl
dc.subject.en	proteomics	pl
dc.description.volume	22	pl
dc.description.number	13	pl
dc.identifier.doi	10.3390/ijms22136816	pl
dc.identifier.eissn	1422-0067	pl
dc.title.journal	International Journal of Molecular Sciences	pl
dc.language.container	eng	pl
dc.affiliation	Wydział Biologii : Instytut Zoologii i Badań Biomedycznych	pl
dc.affiliation	Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego	pl
dc.affiliation	Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznej	pl
dc.affiliation	Pion Prorektora ds. badań naukowych : Małopolskie Centrum Biotechnologii	pl
dc.affiliation	Jagiellonian Interdisciplinary PhD Programme	pl
dc.subtype	Article	pl
dc.identifier.articleid	6816	pl
dc.rights.original	CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0	pl
dc.identifier.project	ROD UJ / OP	pl
.pointsMNiSW	[2021 A]: 140