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Proteomic profiling of ectosomes derived from paired urothelial bladder cancer and normal cells reveals the presence of biologically-relevant molecules

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Proteomic profiling of ectosomes derived from paired urothelial bladder cancer and normal cells reveals the presence of biologically-relevant molecules

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dc.contributor.author Surman, Magdalena [SAP14016164] pl
dc.contributor.author Kędracka-Krok, Sylwia [SAP11016010] pl
dc.contributor.author Jankowska, Urszula [SAP13905509] pl
dc.contributor.author Drożdż, Anna [USOS96343] pl
dc.contributor.author Stępień, Ewa [SAP14015168] pl
dc.contributor.author Przybyło, Małgorzata [SAP11116102] pl
dc.date.accessioned 2021-07-12T16:53:45Z
dc.date.available 2021-07-12T16:53:45Z
dc.date.issued 2021 pl
dc.identifier.issn 1661-6596 pl
dc.identifier.uri https://ruj.uj.edu.pl/xmlui/handle/item/276207
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Proteomic profiling of ectosomes derived from paired urothelial bladder cancer and normal cells reveals the presence of biologically-relevant molecules pl
dc.type JournalArticle pl
dc.abstract.en Protein content of extracellular vesicles (EVs) can modulate different processes during carcinogenesis. Novel proteomic strategies have been applied several times to profile proteins present in exosomes released by urothelial bladder cancer (UBC) cells. However, similar studies have not been conducted so far on another population of EVs, i.e., ectosomes. In the present study we used a shotgun nanoLC–MS/MS proteomic approach to investigate the protein content of ectosomes released in vitro by T-24 UBC cells and HCV-29 normal ureter epithelial cells. In addition, cancer-promoting effects exerted by UBC-derived ectosomes on non-invasive cells in terms of cell proliferation and migratory properties were assessed. In total, 1158 proteins were identified in T-24-derived ectosomes, while HCV-29-derived ectosomes contained a lower number of 259 identified proteins. Qualitative analysis revealed 938 proteins present uniquely in T-24-derived ectosomes, suggesting their potential applications in bladder cancer management as diagnostic and prognostic biomarkers. In addition, T-24-derived ectosomes increased proliferation and motility of recipient cells, likely due to the ectosomal transfer of the identified cancer-promoting molecules. The present study provided a focused identification of biologically relevant proteins in UBC-derived ectosomes, confirming their role in UBC development and progression, and their applicability for further biomarker-oriented studies in preclinical or clinical settings. pl
dc.subject.en biomarkers pl
dc.subject.en bladder cancer pl
dc.subject.en ectosomes pl
dc.subject.en extracellular vesicles pl
dc.subject.en nanoLC-MS/MS pl
dc.subject.en mass spectrometry pl
dc.subject.en proteomics pl
dc.description.volume 22 pl
dc.description.number 13 pl
dc.identifier.doi 10.3390/ijms22136816 pl
dc.identifier.eissn 1422-0067 pl
dc.title.journal International Journal of Molecular Sciences pl
dc.language.container eng pl
dc.affiliation Wydział Biologii : Instytut Zoologii i Badań Biomedycznych pl
dc.affiliation Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznej pl
dc.affiliation Pion Prorektora ds. badań naukowych : Małopolskie Centrum Biotechnologii pl
dc.affiliation Jagiellonian Interdisciplinary PhD Programme pl
dc.subtype Article pl
dc.identifier.articleid 6816 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0 pl
dc.identifier.project ROD UJ / OP pl
.pointsMNiSW [2021 A]: 140


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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa