Silver nanoparticle-based assay for the detection of immunoglobulin free light chains

2019
journal article
article
9
cris.lastimport.wos2024-04-09T18:18:11Z
dc.abstract.enThere is a wide spectrum of malignant diseases that are connected with the clonal proliferation of plasma cells, which cause the production of complete immunoglobulins or their fragments (heavy or light immunoglobulin chains). These proteins may accumulate in tissues, leading to end organ damage. The quantitative determination of immunoglobulin free light chains (FLCs) is considered to be the gold standard in the detection and treatment of multiple myeloma (MM) and amyloid light-chain (AL) amyloidosis. In this study, a silver nanoparticle-based diagnostic tool for the quantitation of FLCs is presented. The optimal test conditions were achieved when a metal nanoparticle (MNP) was covered with 10 particles of an antibody and conjugated by 5-50 protein antigen particles (FLCs). The formation of the second antigen protein corona was accompanied by noticeable changes in the surface plasmon resonance spectra of the silver nanoparticles (AgNPs), which coincided with an increase of the hydrodynamic diameter and increase in the zeta potential, as demonstrated by dynamic light scattering (DLS). A decrease of repulsion forces and the formation of antigen–antibody bridges resulted in the agglutination of AgNPs, as demonstrated by transmission electron microscopy and the direct formation of AgNP aggregates. Antigen-conjugated AgNPs clusters were also found by direct observation using green laser light scattering. The parameters of the specific immunochemical aggregation process consistent with the sizes of AgNPs and the protein particles that coat them were confirmed by four physical methods, yielding complementary data concerning a clinically useful AgNPs aggregation test.pl
dc.affiliationWydział Farmaceutyczny : Zakład Diagnostyki Medycznejpl
dc.cm.date2020-12-02
dc.cm.id96010
dc.contributor.authorLizoń, Anna - 256249 pl
dc.contributor.authorWytrwal-Sarna, Magdalenapl
dc.contributor.authorGajewska, Martapl
dc.contributor.authorDrożdż, Ryszard - 129249 pl
dc.date.accession2019-09-27pl
dc.date.accessioned2020-12-02T10:07:40Zpl
dc.date.available2020-12-02T10:07:40Zpl
dc.date.issued2019pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number18pl
dc.description.points140pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume12pl
dc.identifier.articleid2981pl
dc.identifier.doi10.3390/ma12182981pl
dc.identifier.eissn1996-1944pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/256111
dc.identifier.weblinkhttps://www.mdpi.com/1996-1944/12/18/2981pl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enmultiple myelomapl
dc.subject.enamyloidosispl
dc.subject.enlaser light scatteringpl
dc.subject.ensilver nanoparticlespl
dc.subtypeArticlepl
dc.titleSilver nanoparticle-based assay for the detection of immunoglobulin free light chainspl
dc.title.journalMaterialspl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T18:18:11Z
dc.abstract.enpl
There is a wide spectrum of malignant diseases that are connected with the clonal proliferation of plasma cells, which cause the production of complete immunoglobulins or their fragments (heavy or light immunoglobulin chains). These proteins may accumulate in tissues, leading to end organ damage. The quantitative determination of immunoglobulin free light chains (FLCs) is considered to be the gold standard in the detection and treatment of multiple myeloma (MM) and amyloid light-chain (AL) amyloidosis. In this study, a silver nanoparticle-based diagnostic tool for the quantitation of FLCs is presented. The optimal test conditions were achieved when a metal nanoparticle (MNP) was covered with 10 particles of an antibody and conjugated by 5-50 protein antigen particles (FLCs). The formation of the second antigen protein corona was accompanied by noticeable changes in the surface plasmon resonance spectra of the silver nanoparticles (AgNPs), which coincided with an increase of the hydrodynamic diameter and increase in the zeta potential, as demonstrated by dynamic light scattering (DLS). A decrease of repulsion forces and the formation of antigen–antibody bridges resulted in the agglutination of AgNPs, as demonstrated by transmission electron microscopy and the direct formation of AgNP aggregates. Antigen-conjugated AgNPs clusters were also found by direct observation using green laser light scattering. The parameters of the specific immunochemical aggregation process consistent with the sizes of AgNPs and the protein particles that coat them were confirmed by four physical methods, yielding complementary data concerning a clinically useful AgNPs aggregation test.
dc.affiliationpl
Wydział Farmaceutyczny : Zakład Diagnostyki Medycznej
dc.cm.date
2020-12-02
dc.cm.id
96010
dc.contributor.authorpl
Lizoń, Anna - 256249
dc.contributor.authorpl
Wytrwal-Sarna, Magdalena
dc.contributor.authorpl
Gajewska, Marta
dc.contributor.authorpl
Drożdż, Ryszard - 129249
dc.date.accessionpl
2019-09-27
dc.date.accessionedpl
2020-12-02T10:07:40Z
dc.date.availablepl
2020-12-02T10:07:40Z
dc.date.issuedpl
2019
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
18
dc.description.pointspl
140
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
12
dc.identifier.articleidpl
2981
dc.identifier.doipl
10.3390/ma12182981
dc.identifier.eissnpl
1996-1944
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/256111
dc.identifier.weblinkpl
https://www.mdpi.com/1996-1944/12/18/2981
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
multiple myeloma
dc.subject.enpl
amyloidosis
dc.subject.enpl
laser light scattering
dc.subject.enpl
silver nanoparticles
dc.subtypepl
Article
dc.titlepl
Silver nanoparticle-based assay for the detection of immunoglobulin free light chains
dc.title.journalpl
Materials
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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