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Silver nanoparticle-based assay for the detection of immunoglobulin free light chains
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
Silver nanoparticle-based assay for the detection of immunoglobulin free light chains
Bibliographic description
| title: | Silver nanoparticle-based assay for the detection of immunoglobulin free light chains |
| author: | Lizoń Anna  , Wytrwal-Sarna Magdalena, Gajewska Marta, Drożdż Ryszard
|
| journal title: | Materials |
| volume: | 12 |
| issue: | 18 |
| date of publication : | 2019 |
| article ID: | 2981 |
| eISSN: |
1996-1944 |
| DOI: | |
| URL: | |
| date accessed: |
2019-09-27 |
| language: | English |
| journal language: | English |
| abstract in English: | There is a wide spectrum of malignant diseases that are connected with the clonal proliferation of plasma cells, which cause the production of complete immunoglobulins or their fragments (heavy or light immunoglobulin chains). These proteins may accumulate in tissues, leading to end organ damage. The quantitative determination of immunoglobulin free light chains (FLCs) is considered to be the gold standard in the detection and treatment of multiple myeloma (MM) and amyloid light-chain (AL) amyloidosis. In this study, a silver nanoparticle-based diagnostic tool for the quantitation of FLCs is presented. The optimal test conditions were achieved when a metal nanoparticle (MNP) was covered with 10 particles of an antibody and conjugated by 5-50 protein antigen particles (FLCs). The formation of the second antigen protein corona was accompanied by noticeable changes in the surface plasmon resonance spectra of the silver nanoparticles (AgNPs), which coincided with an increase of the hydrodynamic diameter and increase in the zeta potential, as demonstrated by dynamic light scattering (DLS). A decrease of repulsion forces and the formation of antigen–antibody bridges resulted in the agglutination of AgNPs, as demonstrated by transmission electron microscopy and the direct formation of AgNP aggregates. Antigen-conjugated AgNPs clusters were also found by direct observation using green laser light scattering. The parameters of the specific immunochemical aggregation process consistent with the sizes of AgNPs and the protein particles that coat them were confirmed by four physical methods, yielding complementary data concerning a clinically useful AgNPs aggregation test. |
| keywords in English: | multiple myeloma, amyloidosis, laser light scattering, silver nanoparticles |
| departmental parameterization: | 140 |
| affiliation: | Wydział Farmaceutyczny : Zakład Diagnostyki Medycznej |
| type: | journal article |
| subtype: | academic paper |
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
