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Photocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cells

Photocrosslinked ultrathin anionic polysaccharide ...

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dc.contributor.author Mikulska, Anna pl
dc.contributor.author Filipowska, Joanna pl
dc.contributor.author Osyczka, Anna [SAP11019024] pl
dc.contributor.author Szuwarzyński, Michał [SAP14004038] pl
dc.contributor.author Nowakowska, Maria [SAP11005597] pl
dc.contributor.author Szczubiałka, Krzysztof [SAP11014318] pl
dc.date.accessioned 2015-01-15T09:26:59Z
dc.date.available 2015-01-15T09:26:59Z
dc.date.issued 2014 pl
dc.identifier.issn 0960-7722 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/2550
dc.language eng pl
dc.title Photocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cells pl
dc.type JournalArticle pl
dc.description.physical 516-526 pl
dc.abstract.en Objectives: Properties of cell culture supports obtained from ultrathin multilayer films containing anionic natural polysaccharides (PSacs) and a synthetic polycation were studied. Materials and methods: Supports were prepared via a layer-by-layer (LbL) self-assembly deposition method. Polymers used were: heparin (Hep), chondroitin sulphate (CS), hyaluronic acid (HA), and ι-carrageenan (Car) as polyanions, and diazoresin (DR) as a polycation. PSac layers were crosslinked with DR layers by irradiation with UV light absorbed by DR resin. Results: DR/PSac films are very efficient cell culture growth supports as found from experiments with human mesenchymal stem cells (hMSCs). Irradiation of the films resulted in changing zeta potential of outermost layers of both DR and PSac to more negative values, and in increased film hydrophobicity, as found from the contact angle measurements. Photocrosslinking of the supports led to their increased stability. Conclusions: The supports allow for obtaining intact cell monolayers faster than when typical polystyrene tissue culture plates are used. Moreover, these monolayers spontaneously detach permitting formation of new cell layers on these surfaces relatively early during culture, compared to cells cultured on commonly used tissue culture plastic. pl
dc.description.volume 47 pl
dc.description.number 6 pl
dc.identifier.doi 10.1111/cpr.12118 pl
dc.identifier.eissn 1365-2184 pl
dc.title.journal Cell Proliferation pl
dc.language.container eng pl
dc.affiliation Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2014 A]: 20


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