Photocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cells

doi:10.1111/cpr.12118

Photocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cells

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Photocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cells

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punktacja MEiN [2014 A]: 20

title:	Photocrosslinked ultrathin anionic polysaccharide supports for accelerated growth of human mesenchymal stem cells
author:	Mikulska Anna, Filipowska Joanna, Osyczka Anna , Szuwarzyński Michał , Nowakowska Maria , Szczubiałka Krzysztof
journal title:	Cell Proliferation
volume:	47
issue:	6
date of publication :	2014
pages:	516-526
ISSN:	0960-7722
eISSN:	1365-2184
DOI:	10.1111/cpr.12118
language:	English
journal language:	English
abstract in English:	Objectives: Properties of cell culture supports obtained from ultrathin multilayer films containing anionic natural polysaccharides (PSacs) and a synthetic polycation were studied. Materials and methods: Supports were prepared via a layer-by-layer (LbL) self-assembly deposition method. Polymers used were: heparin (Hep), chondroitin sulphate (CS), hyaluronic acid (HA), and ι-carrageenan (Car) as polyanions, and diazoresin (DR) as a polycation. PSac layers were crosslinked with DR layers by irradiation with UV light absorbed by DR resin. Results: DR/PSac films are very efficient cell culture growth supports as found from experiments with human mesenchymal stem cells (hMSCs). Irradiation of the films resulted in changing zeta potential of outermost layers of both DR and PSac to more negative values, and in increased film hydrophobicity, as found from the contact angle measurements. Photocrosslinking of the supports led to their increased stability. Conclusions: The supports allow for obtaining intact cell monolayers faster than when typical polystyrene tissue culture plates are used. Moreover, these monolayers spontaneously detach permitting formation of new cell layers on these surfaces relatively early during culture, compared to cells cultured on commonly used tissue culture plastic.
affiliation:	Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii, Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
type:	journal article
subtype:	academic paper

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