Characterization of enzymatic activity of MlrB and MlrC proteins involved in bacterial degradation of cyanotoxins microcystins

2016
journal article
article
40
dc.abstract.enBacterial degradation of toxic microcystins produced by cyanobacteria is a common phenomenon. However, our understanding of the mechanisms of these processes is rudimentary. In this paper several novel discoveries regarding the action of the enzymes of the mlr cluster responsible for microcystin biodegradation are presented using recombinant proteins. In particular, the predicted active sites of the recombinant MlrB and MlrC were analyzed using functional enzymes and their inactive muteins. A new degradation intermediate, a hexapeptide derived from linearized microcystins by MlrC, was discovered. Furthermore, the involvement of MlrA and MlrB in further degradation of the hexapeptides was confirmed and a corrected biochemical pathway of microcystin biodegradation has been proposed.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Roślinpl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Fizjologii i Biologii Rozwoju Roślinpl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Analitycznejpl
dc.contributor.authorDziga, Dariusz - 127864 pl
dc.contributor.authorZielińska, Gabriela - 117332 pl
dc.contributor.authorWładyka, Benedykt - 132671 pl
dc.contributor.authorBocheńska, Oliwia - 173990 pl
dc.contributor.authorMaksylewicz, Anna - 255557 pl
dc.contributor.authorStrzałka, Wojciech - 100863 pl
dc.contributor.authorMeriluoto, Jussipl
dc.date.accessioned2016-04-18T11:40:12Z
dc.date.available2016-04-18T11:40:12Z
dc.date.issued2016pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number3pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume8pl
dc.identifier.articleid76pl
dc.identifier.doi10.3390/toxins8030076pl
dc.identifier.eissn2072-6651pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/24254
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enmicrocystinpl
dc.subject.enbiochemical pathwaypl
dc.subject.enbiodegradationpl
dc.subject.enrecombinant enzymespl
dc.subtypeArticlepl
dc.titleCharacterization of enzymatic activity of MlrB and MlrC proteins involved in bacterial degradation of cyanotoxins microcystinspl
dc.title.journalToxinspl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Bacterial degradation of toxic microcystins produced by cyanobacteria is a common phenomenon. However, our understanding of the mechanisms of these processes is rudimentary. In this paper several novel discoveries regarding the action of the enzymes of the mlr cluster responsible for microcystin biodegradation are presented using recombinant proteins. In particular, the predicted active sites of the recombinant MlrB and MlrC were analyzed using functional enzymes and their inactive muteins. A new degradation intermediate, a hexapeptide derived from linearized microcystins by MlrC, was discovered. Furthermore, the involvement of MlrA and MlrB in further degradation of the hexapeptides was confirmed and a corrected biochemical pathway of microcystin biodegradation has been proposed.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Roślin
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Fizjologii i Biologii Rozwoju Roślin
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Analitycznej
dc.contributor.authorpl
Dziga, Dariusz - 127864
dc.contributor.authorpl
Zielińska, Gabriela - 117332
dc.contributor.authorpl
Władyka, Benedykt - 132671
dc.contributor.authorpl
Bocheńska, Oliwia - 173990
dc.contributor.authorpl
Maksylewicz, Anna - 255557
dc.contributor.authorpl
Strzałka, Wojciech - 100863
dc.contributor.authorpl
Meriluoto, Jussi
dc.date.accessioned
2016-04-18T11:40:12Z
dc.date.available
2016-04-18T11:40:12Z
dc.date.issuedpl
2016
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
3
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
8
dc.identifier.articleidpl
76
dc.identifier.doipl
10.3390/toxins8030076
dc.identifier.eissnpl
2072-6651
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/24254
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
microcystin
dc.subject.enpl
biochemical pathway
dc.subject.enpl
biodegradation
dc.subject.enpl
recombinant enzymes
dc.subtypepl
Article
dc.titlepl
Characterization of enzymatic activity of MlrB and MlrC proteins involved in bacterial degradation of cyanotoxins microcystins
dc.title.journalpl
Toxins
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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