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Characterization of enzymatic activity of MlrB and MlrC proteins involved in bacterial degradation of cyanotoxins microcystins
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
Characterization of enzymatic activity of MlrB and MlrC proteins involved in bacterial degradation of cyanotoxins microcystins
Bibliographic description
| title: | Characterization of enzymatic activity of MlrB and MlrC proteins involved in bacterial degradation of cyanotoxins microcystins |
| author: | Dziga Dariusz  , Zielińska Gabriela , Władyka Benedykt , Bocheńska Oliwia , Maksylewicz Anna , Strzałka Wojciech , Meriluoto Jussi |
| journal title: | Toxins |
| volume: | 8 |
| issue: | 3 |
| date of publication : | 2016 |
| article ID: | 76 |
| eISSN: |
2072-6651 |
| DOI: | |
| language: | English |
| journal language: | English |
| abstract in English: | Bacterial degradation of toxic microcystins produced by cyanobacteria is a common phenomenon. However, our understanding of the mechanisms of these processes is rudimentary. In this paper several novel discoveries regarding the action of the enzymes of the mlr cluster responsible for microcystin biodegradation are presented using recombinant proteins. In particular, the predicted active sites of the recombinant MlrB and MlrC were analyzed using functional enzymes and their inactive muteins. A new degradation intermediate, a hexapeptide derived from linearized microcystins by MlrC, was discovered. Furthermore, the involvement of MlrA and MlrB in further degradation of the hexapeptides was confirmed and a corrected biochemical pathway of microcystin biodegradation has been proposed. |
| keywords in English: | microcystin, biochemical pathway, biodegradation, recombinant enzymes |
| affiliation: | Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Roślin, Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Fizjologii i Biologii Rozwoju Roślin, Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Analitycznej |
| type: | journal article |
| subtype: | academic paper |
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
