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Endothelial precursor cell-based therapy to target the pathologic angiogenesis and compensate tumor hypoxia
endothelial precursor cells
pathologic angiogenesis/vasculogenesis
tumor targeting
cell gene therapy
Hypoxia-inducing pathologies as cancer develop pathologic and inefficient angiogenesis which rules tumor facilitating microenvironment, a key target for therapy. As such, the putative ability of endothelial precursor cells (EPCs) to specifically home to hypoxic sites of neovascularization prompted to design optimized, site-specific, cell-mediated, drug-/gene-targeting approach. Thus, EPC lines were established from aorta–gonad–mesonephros (AGM) of murine 10.5 dpc and 11.5 dpc embryo when endothelial repertoire is completed. Lines representing early endothelial differentiation steps were selected: MAgEC10.5 and MagEC11.5. Distinct in maturation, they differently express VEGF receptors, VE-cadherin and chemokine/receptors. MAgEC11.5, more differentiated than MAgEC 10.5, displayed faster angiogenesis in vitro, different response to hypoxia and chemokines. Both MAgEC lines cooperated to tube-like formation with mature endothelial cells and invaded tumor spheroids through a vasculogenesis-like process. In vivo, both MAgEC-formed vessels established blood flow. Intravenously injected, both MAgECs invaded MatrigelTM-plugs and targeted tumors. Here we show that EPCs (MAgEC11.5) target tumor angiogenesis and allow local overexpression of hypoxia-driven soluble VEGF-receptor2 enabling drastic tumor growth reduction. We propose that such EPCs, able to target tumor angiogenesis, could act as therapeutic gene vehicles to inhibit tumor growth by vessel normalization resulting from tumor hypoxia alleviation.
dc.abstract.en | Hypoxia-inducing pathologies as cancer develop pathologic and inefficient angiogenesis which rules tumor facilitating microenvironment, a key target for therapy. As such, the putative ability of endothelial precursor cells (EPCs) to specifically home to hypoxic sites of neovascularization prompted to design optimized, site-specific, cell-mediated, drug-/gene-targeting approach. Thus, EPC lines were established from aorta–gonad–mesonephros (AGM) of murine 10.5 dpc and 11.5 dpc embryo when endothelial repertoire is completed. Lines representing early endothelial differentiation steps were selected: MAgEC10.5 and MagEC11.5. Distinct in maturation, they differently express VEGF receptors, VE-cadherin and chemokine/receptors. MAgEC11.5, more differentiated than MAgEC 10.5, displayed faster angiogenesis in vitro, different response to hypoxia and chemokines. Both MAgEC lines cooperated to tube-like formation with mature endothelial cells and invaded tumor spheroids through a vasculogenesis-like process. In vivo, both MAgEC-formed vessels established blood flow. Intravenously injected, both MAgECs invaded MatrigelTM-plugs and targeted tumors. Here we show that EPCs (MAgEC11.5) target tumor angiogenesis and allow local overexpression of hypoxia-driven soluble VEGF-receptor2 enabling drastic tumor growth reduction. We propose that such EPCs, able to target tumor angiogenesis, could act as therapeutic gene vehicles to inhibit tumor growth by vessel normalization resulting from tumor hypoxia alleviation. | pl |
dc.affiliation | Pion Prorektora ds. badań naukowych i funduszy strukturalnych : Małopolskie Centrum Biotechnologii | pl |
dc.affiliation | Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Medycznej | pl |
dc.contributor.author | Collet, Guillaume | pl |
dc.contributor.author | Szade, Krzysztof - 109224 | pl |
dc.contributor.author | Nowak, Witold - 108796 | pl |
dc.contributor.author | Klimkiewicz, Krzysztof - 114597 | pl |
dc.contributor.author | El Hafny-Rahbi, Bouchra | pl |
dc.contributor.author | Szczepanek, Karol - 137332 | pl |
dc.contributor.author | Sugiyama, Daisuke | pl |
dc.contributor.author | Weglarczyk, Kazimierz | pl |
dc.contributor.author | Foucault-Collet, Alexandra | pl |
dc.contributor.author | Guichard, Alan | pl |
dc.contributor.author | Mazan, Andrzej | pl |
dc.contributor.author | Nadim, Mahdi | pl |
dc.contributor.author | Fasani, Fabienne | pl |
dc.contributor.author | Lamerant-Fayel, Nathalie | pl |
dc.contributor.author | Grillon, Catherine | pl |
dc.contributor.author | Petoud, Stéphane | pl |
dc.contributor.author | Beloeil, Jean-Claude | pl |
dc.contributor.author | Józkowicz, Alicja - 128541 | pl |
dc.contributor.author | Dulak, Józef - 127818 | pl |
dc.contributor.author | Kieda, Claudine - 255953 | pl |
dc.date.accessioned | 2016-04-11T08:54:44Z | |
dc.date.available | 2016-04-11T08:54:44Z | |
dc.date.issued | 2016 | pl |
dc.date.openaccess | 0 | |
dc.description.accesstime | w momencie opublikowania | |
dc.description.number | 2 | pl |
dc.description.physical | 345-357 | pl |
dc.description.version | ostateczna wersja wydawcy | |
dc.description.volume | 370 | pl |
dc.identifier.doi | 10.1016/j.canlet.2015.11.008 | pl |
dc.identifier.eissn | 1872-7980 | pl |
dc.identifier.issn | 0304-3835 | pl |
dc.identifier.project | ROD UJ / P | pl |
dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/23747 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 Międzynarodowa | * |
dc.rights.licence | CC-BY-NC-ND | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode.pl | * |
dc.share.type | inne | |
dc.subject.en | endothelial precursor cells | pl |
dc.subject.en | pathologic angiogenesis/vasculogenesis | pl |
dc.subject.en | tumor targeting | pl |
dc.subject.en | cell gene therapy | pl |
dc.subtype | Article | pl |
dc.title | Endothelial precursor cell-based therapy to target the pathologic angiogenesis and compensate tumor hypoxia | pl |
dc.title.journal | Cancer Letters | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |
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