Development of insect resistant maize plants expressing a chitinase gene from the cotton leaf worm, Spodoptera littoralis

2015
journal article
article
41
cris.lastimport.wos2024-04-09T21:57:51Z
dc.abstract.enDue to the importance of chitinolytic enzymes for insect, nematode and fungal growth, they are receiving attention concerning their development as biopesticides or chemical defense proteins in transgenic plants and as microbial biocontrol agents. Targeting chitin associated with the extracellular matrices or cell wall by insect chitinases may be an effective approach for controlling pest insects and pathogenic fungi. The ability of chitinases to attack and digest chitin in the peritrophic matrix or exoskeleton raises the possibility to use them as insect control method. In this study, an insect chitinase cDNA from cotton leaf worm (Spodoptera littoralis) has been synthesized. Transgenic maize plant system was used to improve its tolerance against insects. Insect chitinase transcripts and proteins were expressed in transgenic maize plants. The functional integrity and expression of chitinase in progenies of the transgenic plants were confirmed by insect bioassays. The bioassays using transgenic corn plants against corn borer (Sesamia cretica) revealed that ~50% of the insects reared on transgenic corn plants died, suggesting that transgenic maize plants have enhanced resistance against S. cretica.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Komórkipl
dc.contributor.authorOsman, Gamal H.pl
dc.contributor.authorAssem, Shireen K.pl
dc.contributor.authorAlreedy, Rasha M.pl
dc.contributor.authorEl-Ghareeb, Doaa K.pl
dc.contributor.authorBasry, Mahmoud A.pl
dc.contributor.authorRastogi, Anshu - 238508 pl
dc.contributor.authorKalaji, Hazem M.pl
dc.date.accessioned2016-02-25T10:15:02Z
dc.date.available2016-02-25T10:15:02Z
dc.date.issued2015pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.versionostateczna wersja wydawcy
dc.description.volume5pl
dc.identifier.articleid18067pl
dc.identifier.doi10.1038/srep18067pl
dc.identifier.eissn2045-2322pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/21434
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subtypeArticlepl
dc.titleDevelopment of insect resistant maize plants expressing a chitinase gene from the cotton leaf worm, Spodoptera littoralispl
dc.title.journalScientific Reportspl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T21:57:51Z
dc.abstract.enpl
Due to the importance of chitinolytic enzymes for insect, nematode and fungal growth, they are receiving attention concerning their development as biopesticides or chemical defense proteins in transgenic plants and as microbial biocontrol agents. Targeting chitin associated with the extracellular matrices or cell wall by insect chitinases may be an effective approach for controlling pest insects and pathogenic fungi. The ability of chitinases to attack and digest chitin in the peritrophic matrix or exoskeleton raises the possibility to use them as insect control method. In this study, an insect chitinase cDNA from cotton leaf worm (Spodoptera littoralis) has been synthesized. Transgenic maize plant system was used to improve its tolerance against insects. Insect chitinase transcripts and proteins were expressed in transgenic maize plants. The functional integrity and expression of chitinase in progenies of the transgenic plants were confirmed by insect bioassays. The bioassays using transgenic corn plants against corn borer (Sesamia cretica) revealed that ~50% of the insects reared on transgenic corn plants died, suggesting that transgenic maize plants have enhanced resistance against S. cretica.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Komórki
dc.contributor.authorpl
Osman, Gamal H.
dc.contributor.authorpl
Assem, Shireen K.
dc.contributor.authorpl
Alreedy, Rasha M.
dc.contributor.authorpl
El-Ghareeb, Doaa K.
dc.contributor.authorpl
Basry, Mahmoud A.
dc.contributor.authorpl
Rastogi, Anshu - 238508
dc.contributor.authorpl
Kalaji, Hazem M.
dc.date.accessioned
2016-02-25T10:15:02Z
dc.date.available
2016-02-25T10:15:02Z
dc.date.issuedpl
2015
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
5
dc.identifier.articleidpl
18067
dc.identifier.doipl
10.1038/srep18067
dc.identifier.eissnpl
2045-2322
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/21434
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subtypepl
Article
dc.titlepl
Development of insect resistant maize plants expressing a chitinase gene from the cotton leaf worm, Spodoptera littoralis
dc.title.journalpl
Scientific Reports
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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