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Development of insect resistant maize plants expressing a chitinase gene from the cotton leaf worm, Spodoptera littoralis
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
Development of insect resistant maize plants expressing a chitinase gene from the cotton leaf worm, Spodoptera littoralis
Bibliographic description
| title: | Development of insect resistant maize plants expressing a chitinase gene from the cotton leaf worm, Spodoptera littoralis |
| author: | Osman Gamal H., Assem Shireen K., Alreedy Rasha M., El-Ghareeb Doaa K., Basry Mahmoud A., Rastogi Anshu  , Kalaji Hazem M. |
| journal title: | Scientific Reports |
| volume: | 5 |
| date of publication : | 2015 |
| article ID: | 18067 |
| eISSN: |
2045-2322 |
| DOI: | |
| language: | English |
| journal language: | English |
| abstract in English: | Due to the importance of chitinolytic enzymes for insect, nematode and fungal growth, they are receiving attention concerning their development as biopesticides or chemical defense proteins in transgenic plants and as microbial biocontrol agents. Targeting chitin associated with the extracellular matrices or cell wall by insect chitinases may be an effective approach for controlling pest insects and pathogenic fungi. The ability of chitinases to attack and digest chitin in the peritrophic matrix or exoskeleton raises the possibility to use them as insect control method. In this study, an insect chitinase cDNA from cotton leaf worm (Spodoptera littoralis) has been synthesized. Transgenic maize plant system was used to improve its tolerance against insects. Insect chitinase transcripts and proteins were expressed in transgenic maize plants. The functional integrity and expression of chitinase in progenies of the transgenic plants were confirmed by insect bioassays. The bioassays using transgenic corn plants against corn borer (Sesamia cretica) revealed that ~50% of the insects reared on transgenic corn plants died, suggesting that transgenic maize plants have enhanced resistance against S. cretica. |
| affiliation: | Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Komórki |
| type: | journal article |
| subtype: | academic paper |
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
