A three-marker DNA barcoding approach for ecological studies of xerothermic plants and herbivorous insects from central Europe

2015
journal article
article
14
cris.lastimport.wos2024-04-09T20:09:55Z
dc.abstract.enThe DNA barcoding technique developed for species identification has recently been adapted for ecological studies (e.g. host plant identification). Comprehensive barcode databases, covering most species inhabiting areas, habitats or communities of interest are essential for reliable and efficient identification of plants. Here we present a three-barcode (plastid rbcL and matK genes and the trnL intron) database for xerothermic plant species from central Europe. About 85% of the xerothermic plant species (126 out of c. 150) known to be associated with xerothermic habitats were collected and barcoded. The database contains barcodes for 117 (rbcL and trnL) and 96 (matK) species. Interspecific nucleotide distances were in the ranges 0–17.9% (0–3.2% within genera) for rbcL, 0–44.4% (0–3.1%) for trnL and 0–52.5% (0–10.9%) for matK. Blast-searching of each sequence in the database against the entire database showed that species-level identification is possible for 89.6% (rbcL), 98.4% (trnL) and 96.4% (matK) of examined plant species. The utility of the presented database for identification of host plants was demonstrated using two insect species associated with xerothermic habitats: the oligophagous leaf-beetle Cheilotoma musciformis (for which two host plants in Fabaceae were identified) and the polyphagous weevil Polydrusus inustus (which was found to feed on 14 host plants, mostly Rosaceae, Asteraceae and Fabaceae). The developed database will be useful in various applications, including biodiversity, phylogeography, conservation and ecologypl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Nauk o Środowiskupl
dc.affiliationWydział Biologii i Nauk o Ziemi : Instytut Botanikipl
dc.contributor.authorHeise, Waldemar - 159882 pl
dc.contributor.authorBabik, Wiesław - 127155 pl
dc.contributor.authorKubisz, Danielpl
dc.contributor.authorKajtoch, Łukaszpl
dc.date.accessioned2016-01-11T14:09:14Z
dc.date.available2016-01-11T14:09:14Z
dc.date.issued2015pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number4pl
dc.description.physical576-592pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume177pl
dc.identifier.doi10.1111/boj.12261pl
dc.identifier.eissn1095-8339pl
dc.identifier.issn0024-4074pl
dc.identifier.projectROD UJ / Ppl
dc.identifier.urihttp://ruj.uj.edu.pl/xmlui/handle/item/19208
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne*
dc.rights.licenceCC-BY-NC
dc.rights.urihttps://creativecommons.org/licenses*
dc.share.typeotwarte czasopismo
dc.subject.encalcareous grasslandspl
dc.subject.enColeopterapl
dc.subject.endry grasslandspl
dc.subject.enmatKpl
dc.subject.enplastid DNApl
dc.subject.enrbcLpl
dc.subject.entrnLpl
dc.subtypeArticlepl
dc.titleA three-marker DNA barcoding approach for ecological studies of xerothermic plants and herbivorous insects from central Europepl
dc.title.journalBotanical Journal of the Linnean Societypl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T20:09:55Z
dc.abstract.enpl
The DNA barcoding technique developed for species identification has recently been adapted for ecological studies (e.g. host plant identification). Comprehensive barcode databases, covering most species inhabiting areas, habitats or communities of interest are essential for reliable and efficient identification of plants. Here we present a three-barcode (plastid rbcL and matK genes and the trnL intron) database for xerothermic plant species from central Europe. About 85% of the xerothermic plant species (126 out of c. 150) known to be associated with xerothermic habitats were collected and barcoded. The database contains barcodes for 117 (rbcL and trnL) and 96 (matK) species. Interspecific nucleotide distances were in the ranges 0–17.9% (0–3.2% within genera) for rbcL, 0–44.4% (0–3.1%) for trnL and 0–52.5% (0–10.9%) for matK. Blast-searching of each sequence in the database against the entire database showed that species-level identification is possible for 89.6% (rbcL), 98.4% (trnL) and 96.4% (matK) of examined plant species. The utility of the presented database for identification of host plants was demonstrated using two insect species associated with xerothermic habitats: the oligophagous leaf-beetle Cheilotoma musciformis (for which two host plants in Fabaceae were identified) and the polyphagous weevil Polydrusus inustus (which was found to feed on 14 host plants, mostly Rosaceae, Asteraceae and Fabaceae). The developed database will be useful in various applications, including biodiversity, phylogeography, conservation and ecology
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Nauk o Środowisku
dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Botaniki
dc.contributor.authorpl
Heise, Waldemar - 159882
dc.contributor.authorpl
Babik, Wiesław - 127155
dc.contributor.authorpl
Kubisz, Daniel
dc.contributor.authorpl
Kajtoch, Łukasz
dc.date.accessioned
2016-01-11T14:09:14Z
dc.date.available
2016-01-11T14:09:14Z
dc.date.issuedpl
2015
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
4
dc.description.physicalpl
576-592
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
177
dc.identifier.doipl
10.1111/boj.12261
dc.identifier.eissnpl
1095-8339
dc.identifier.issnpl
0024-4074
dc.identifier.projectpl
ROD UJ / P
dc.identifier.uri
http://ruj.uj.edu.pl/xmlui/handle/item/19208
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne
dc.rights.licence
CC-BY-NC
dc.rights.uri*
https://creativecommons.org/licenses
dc.share.type
otwarte czasopismo
dc.subject.enpl
calcareous grasslands
dc.subject.enpl
Coleoptera
dc.subject.enpl
dry grasslands
dc.subject.enpl
matK
dc.subject.enpl
plastid DNA
dc.subject.enpl
rbcL
dc.subject.enpl
trnL
dc.subtypepl
Article
dc.titlepl
A three-marker DNA barcoding approach for ecological studies of xerothermic plants and herbivorous insects from central Europe
dc.title.journalpl
Botanical Journal of the Linnean Society
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

* The migration of download and view statistics prior to the date of April 8, 2024 is in progress.