Vanadium compounds affect growth and morphology of human rhabdomyosarcoma cell line

2011
journal article
article
dc.abstract.enRhabdomyosarcoma (RMS) cells were incubated with four vanadium compounds: cations BMOV and vanadyl sulphate, and anions ortho- and metavanadate. Growth inhibition of RMS cells in the culture was determined by two staining methods: with N-hexamethylpararosaniline (crystal violet = CV) or bromide 3-(4,5- dimethylthiazol-2)-2,5-diphenyltetrazolium (MTT). After 48 h incubation with 10-40 μM for NaVO3 or 20-40 μM for the other three vanadium salts, the results were statistically significantly lower (0.001 < p < 0.01) as compared to the controls (without vanadium in the medium). A vanadium concentration higher than 40 μM resulted in cell destruction or death in all cells. A comparison with our previously obtained results showed the greatest sensitivity of rat hepatoma H 35-19 cells in comparison to four human cancer cell lines (A549, DU145, HTB, RMS). Investigations of human cancer cells demonstrated that the highest resistance to orthovanadate was characteristic of RMS (c.40 μM) and HTB (c. 20 μM). Electron microscopic examination showed pleomorphic nuclei with visible amounts of heterochromatin and large nucleoli, characteristic of RMS cells. Cells at various stages of differentiation were observed.pl
dc.affiliationWydział Lekarskipl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Ogólnejpl
dc.contributor.authorDąbroś, Wojciech - 129148 pl
dc.contributor.authorAdamczyk, Anna - 108279 pl
dc.contributor.authorCiurkot, Katarzynapl
dc.contributor.authorKordowiak, Anna - 129114 pl
dc.date.accession2020-04-27pl
dc.date.accessioned2020-04-27T08:04:37Z
dc.date.available2020-04-27T08:04:37Z
dc.date.issued2011pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBibliogr. s. 267-268.pl
dc.description.number4pl
dc.description.physical262-268pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume62pl
dc.identifier.eissn2084-9869pl
dc.identifier.issn1233-9687pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/154609
dc.identifier.weblinkhttps://www.termedia.pl/Vanadium-compounds-affect-growth-and-morphology-of-human-rhabdomyosarcoma-cell-line,55,18080,1,1.htmlpl
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Na tych samych warunkach 4.0 Międzynarodowa*
dc.rights.licenceCC-BY-NC-SA
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.enhuman cancer cellspl
dc.subject.enautocrine growth and morphology of RMS cellspl
dc.subject.envanadyl sulphate (VOSO_4)pl
dc.subject.enBMOV = bis(maltolato)oxovanadiumpl
dc.subject.ensodium orthovanadate (Na_3VO_4)pl
dc.subject.ensodium metavanadate (NaVO_3)pl
dc.subtypeArticlepl
dc.titleVanadium compounds affect growth and morphology of human rhabdomyosarcoma cell linepl
dc.title.journalPolish Journal of Pathologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
dc.abstract.enpl
Rhabdomyosarcoma (RMS) cells were incubated with four vanadium compounds: cations BMOV and vanadyl sulphate, and anions ortho- and metavanadate. Growth inhibition of RMS cells in the culture was determined by two staining methods: with N-hexamethylpararosaniline (crystal violet = CV) or bromide 3-(4,5- dimethylthiazol-2)-2,5-diphenyltetrazolium (MTT). After 48 h incubation with 10-40 μM for NaVO3 or 20-40 μM for the other three vanadium salts, the results were statistically significantly lower (0.001 < p < 0.01) as compared to the controls (without vanadium in the medium). A vanadium concentration higher than 40 μM resulted in cell destruction or death in all cells. A comparison with our previously obtained results showed the greatest sensitivity of rat hepatoma H 35-19 cells in comparison to four human cancer cell lines (A549, DU145, HTB, RMS). Investigations of human cancer cells demonstrated that the highest resistance to orthovanadate was characteristic of RMS (c.40 μM) and HTB (c. 20 μM). Electron microscopic examination showed pleomorphic nuclei with visible amounts of heterochromatin and large nucleoli, characteristic of RMS cells. Cells at various stages of differentiation were observed.
dc.affiliationpl
Wydział Lekarski
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Ogólnej
dc.contributor.authorpl
Dąbroś, Wojciech - 129148
dc.contributor.authorpl
Adamczyk, Anna - 108279
dc.contributor.authorpl
Ciurkot, Katarzyna
dc.contributor.authorpl
Kordowiak, Anna - 129114
dc.date.accessionpl
2020-04-27
dc.date.accessioned
2020-04-27T08:04:37Z
dc.date.available
2020-04-27T08:04:37Z
dc.date.issuedpl
2011
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Bibliogr. s. 267-268.
dc.description.numberpl
4
dc.description.physicalpl
262-268
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
62
dc.identifier.eissnpl
2084-9869
dc.identifier.issnpl
1233-9687
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/154609
dc.identifier.weblinkpl
https://www.termedia.pl/Vanadium-compounds-affect-growth-and-morphology-of-human-rhabdomyosarcoma-cell-line,55,18080,1,1.html
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Na tych samych warunkach 4.0 Międzynarodowa
dc.rights.licence
CC-BY-NC-SA
dc.rights.uri*
http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
human cancer cells
dc.subject.enpl
autocrine growth and morphology of RMS cells
dc.subject.enpl
vanadyl sulphate (VOSO_4)
dc.subject.enpl
BMOV = bis(maltolato)oxovanadium
dc.subject.enpl
sodium orthovanadate (Na_3VO_4)
dc.subject.enpl
sodium metavanadate (NaVO_3)
dc.subtypepl
Article
dc.titlepl
Vanadium compounds affect growth and morphology of human rhabdomyosarcoma cell line
dc.title.journalpl
Polish Journal of Pathology
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

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