Rhabdomyosarcoma (RMS) cells were incubated with four vanadium compounds:
cations BMOV and vanadyl sulphate, and anions ortho- and metavanadate.
Growth inhibition of RMS cells in the culture was determined by two staining methods:
with N-hexamethylpararosaniline (crystal violet = CV) or bromide 3-(4,5-
dimethylthiazol-2)-2,5-diphenyltetrazolium (MTT). After 48 h incubation with
10-40 μM for NaVO3 or 20-40 μM for the other three vanadium salts, the results
were statistically significantly lower (0.001 < p < 0.01) as compared to the controls
(without vanadium in the medium). A vanadium concentration higher than
40 μM resulted in cell destruction or death in all cells. A comparison with our previously
obtained results showed the greatest sensitivity of rat hepatoma H 35-19 cells
in comparison to four human cancer cell lines (A549, DU145, HTB, RMS). Investigations
of human cancer cells demonstrated that the highest resistance to orthovanadate
was characteristic of RMS (c.40 μM) and HTB (c. 20 μM).
Electron microscopic examination showed pleomorphic nuclei with visible amounts
of heterochromatin and large nucleoli, characteristic of RMS cells. Cells at various
stages of differentiation were observed.
keywords in English:
human cancer cells, autocrine growth and morphology of RMS cells, vanadyl sulphate (VOSO_4), BMOV = bis(maltolato)oxovanadium, sodium orthovanadate (Na_3VO_4), sodium metavanadate (NaVO_3)
affiliation:
Wydział Lekarski, Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Ogólnej
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