Increased nitric oxide availability attenuates high fat diet metabolic alterations and gene expression associated with insulin resistance

Increased nitric oxide availability attenuates high fat diet metabolic alterations and gene expression associated with insulin resistance

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Increased nitric oxide availability attenuates high fat diet metabolic alterations and gene expression associated with insulin resistance

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punktacja MNiSW [2011 A]: 30

title:	Increased nitric oxide availability attenuates high fat diet metabolic alterations and gene expression associated with insulin resistance
author:	Raźny Urszula , Kieć-Wilk Beata , Wątor Łukasz , Polus Anna , Dyduch Grzegorz , Solnica Bogdan , Małecki Maciej , Tomaszewska Romana , Cooke John P, Dembińska-Kieć Aldona
journal title:	Cardiovascular Diabetology
volume:	10
date of publication :	2011
article ID:	68
ISSN:	1475-2840
eISSN:	1475-2840
URL:	http://www.cardiab.com/content/10/1/68
date accessed:	2020-04-03
language:	English
journal language:	English
abstract in English:	Background High fat diet impairs nitric oxide (NO) bioavailability, and induces insulin resistance. The link between NO availability and the metabolic adaptation to a high fat diet is not well characterized. The purpose of this study was to investigate the effect of high fat diet on metabolism in mice with decreased (eNOS-/-) and increased (DDAH overexpressed) NO bioavailability. Methods eNOS-/- (n = 16), DDAH (n = 24), and WT (n = 19) mice were fed a high fat diet (HFD) for 13 weeks. Body weight, biochemical parameters, adipokines and insulin were monitored. The matrigel in vivo model with CD31 immunostaining was used to assess angiogenesis. Gene expression in adipose tissues was analyzed by microarray and Real Time PCR. Comparisons of the mean values were made using the unpaired Student t test and p < 0.05 were considered statistically significant. Results eNOS-/- mice gained less weight than control WT and DDAH mice. In DDAH mice, a greater increase in serum adiponectin and a lesser increment in glucose level was observed. Fasting insulin and cholesterol levels remained unchanged. The angiogenic response was increased in DDAH mice. In adipose tissue of DDAH mice, genes characteristic of differentiated adipocytes were down-regulated, whereas in eNOS-/- mice, genes associated with adipogenesis, fatty acid and triglyceride synthesis were upregulated. Conclusions Our results indicate that increased NO availability attenuates some HFD induced alterations in metabolism and gene expression associated with insulin resistance.
keywords in English:	eNOS-/- mice, DDAH mice, microarray, adipogenesis, angiogenesis
number of pulisher's sheets:	1,34
affiliation:	Wydział Lekarski : Klinika Chorób Metabolicznych, Wydział Lekarski : Zakład Diagnostyki, Wydział Lekarski : Zakład Patomorfologii Klinicznej i Doświadczalnej, Wydział Lekarski : Zakład Diagnostyki Genetycznej i Nutrigenomiki, Wydział Lekarski : Zakład Biochemii Klinicznej
type:	journal article
subtype:	academic paper