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Mechanistic studies on the reversible binding of nitric oxide to metmyoglobin
The ferriheme protein metmyoglobin (metMb) in buffer solution at physiological pH 7.4 reversibly
binds the biomessenger molecule nitric oxide to yield the nitrosyl adduct (metMb(NO)). The kinetics of the
association and dissociation processes were investigated by both laser flash photolysis and stopped-flow kinetics
techniques at ambient and high pressure, in three laboratories using several different sources of metMb. The
activation parameters
cris.lastimport.wos | 2024-04-10T01:52:02Z | |
dc.abstract.en | The ferriheme protein metmyoglobin (metMb) in buffer solution at physiological pH 7.4 reversibly binds the biomessenger molecule nitric oxide to yield the nitrosyl adduct (metMb(NO)). The kinetics of the association and dissociation processes were investigated by both laser flash photolysis and stopped-flow kinetics techniques at ambient and high pressure, in three laboratories using several different sources of metMb. The activation parameters $\Delta$H⧧, $\Delta$S⧧, and $\Delta$V⧧ were calculated from the kinetic effects of varying temperature and hydrostatic pressure. For the "on" reaction of metMb plus NO, reasonable agreement was found between the various techniques with $\Delta$$H_{on}$⧧, $\Delta$$S_{on}$⧧, and $\Delta$$V_{on}$⧧ determined to have the respective values $\sim65$ kJ $mol^{-1}$, $\sim60$ J $mol^{-1}$ $K^{-1}$, and $\sim20$ $cm^{3}$ $mol^{-1}$. The large and positive $\Delta$S⧧ and $\Delta$V⧧ values are consistent with the operation of a limiting dissociative ligand substitution mechanism whereby dissociation of the $H_{2}O$ occupying the sixth distal coordination site of metMb must precede formation of the Fe-NO bond. While the activation enthalpies of the "off" reaction displayed reasonable agreement between the various techniques (ranging from 68 to 83 kJ $mol^{-1}$), poorer agreement was found for the $\Delta$$S_{off}$⧧ values. For this reason, the kinetics for the "off" reaction were determined more directly via NO trapping experiments, which gave the respective activation parameters $\Delta$$H_{off}$⧧ = 76 kJ $mol^{-1}$, $\Delta$$S_{off}$⧧ = $\sim41$ J $mol^{-1}$ $K^{-1}$, and $\Delta$$V_{off}$⧧ = 20 $cm^{3}$ $mol^{-1}$), again consistent with a limiting dissociative mechanism. These results are discussed in reference to other investigations of the reactions of NO with both model systems and metalloproteins. | pl |
dc.affiliation | Wydział Chemii : Zakład Chemii Nieorganicznej | pl |
dc.contributor.author | Laverman, Leroy E. | pl |
dc.contributor.author | Wanat, Alicja | pl |
dc.contributor.author | Oszajca, Janusz | pl |
dc.contributor.author | Stochel, Grażyna - 132108 | pl |
dc.contributor.author | Ford, Peter C. | pl |
dc.contributor.author | van Eldik, Rudi - 239234 | pl |
dc.date.accessioned | 2015-08-26T10:15:18Z | |
dc.date.available | 2015-08-26T10:15:18Z | |
dc.date.issued | 2001 | pl |
dc.description.admin | [AU] van Eldik, Rudi [SAP14007947] | pl |
dc.description.number | 2 | pl |
dc.description.physical | 285-293 | pl |
dc.description.volume | 123 | pl |
dc.identifier.doi | 10.1021/ja001696z | pl |
dc.identifier.eissn | 1520-5126 | pl |
dc.identifier.issn | 0002-7863 | pl |
dc.identifier.uri | http://ruj.uj.edu.pl/xmlui/handle/item/15048 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Dodaję tylko opis bibliograficzny | * |
dc.rights.licence | Bez licencji otwartego dostępu | |
dc.rights.uri | * | |
dc.subtype | Article | pl |
dc.title | Mechanistic studies on the reversible binding of nitric oxide to metmyoglobin | pl |
dc.title.journal | Journal of the American Chemical Society | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |