author: |
Asp Julia, Skov Vibe, Bellosillo Beatriz, Kristensen Thomas, Lippert Eric, Dicker Frank, Schwarz Jiri, Wojtaszewska Marzena, Akiki Susanna, Aggerholm Anni, Andersen Morten Tolstrup, Girodon Francois, Kjaer Lasse, Oppliger Leibundgut Elisabeth, Pancrazzi Alessandro, Vorland Marta, Andrikovics Hajnalka, Kralovics Robert, Cassinat Bruno, Coucelo Margarida, Eftimov Aleksandar, Haslam Karl, Kusec Rajko, Link-Lenczowska Dorota, Lode Laurence, Matiakowska Karolina, Naguib Dina, Navaglia Filippo, Novotny Guy Wayne, Percy Melanie J., Sudarikov Andrey, Hermouet Sylvie, Pallisgaard Niels |
abstract in English: |
External quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is
important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be
more accurate than limits of the technology used, and it is essential to recognize factors causing substantial outlier results. The
present study aimed to identify parameters of specific importance for JAK2 V617F quantification by quantitative PCR, using
different starting materials, assays, and technical platforms. Sixteen samples were issued to participating laboratories in two EQA
rounds. In the first round, 19 laboratories from 11 European countries analyzing JAK2 V617F as part of their routine diagnostics
returned results from in-house assays. In the second round, 25 laboratories from 17 countries participated. Despite variations in
starting material, assay set-up and instrumentation the laboratories were generally well aligned in the EQA program. However,
EQA based on a single technology appears to be a valuable tool to achieve standardization of the quantification of JAK2 V617F
allelic burden. |