International external quality assurance of "JAK2 V617F" quantification

2019
journal article
article
7
cris.lastimport.wos2024-04-09T20:08:40Z
dc.abstract.enExternal quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be more accurate than limits of the technology used, and it is essential to recognize factors causing substantial outlier results. The present study aimed to identify parameters of specific importance for JAK2 V617F quantification by quantitative PCR, using different starting materials, assays, and technical platforms. Sixteen samples were issued to participating laboratories in two EQA rounds. In the first round, 19 laboratories from 11 European countries analyzing JAK2 V617F as part of their routine diagnostics returned results from in-house assays. In the second round, 25 laboratories from 17 countries participated. Despite variations in starting material, assay set-up and instrumentation the laboratories were generally well aligned in the EQA program. However, EQA based on a single technology appears to be a valuable tool to achieve standardization of the quantification of JAK2 V617F allelic burden.pl
dc.affiliationWydział Lekarskipl
dc.cm.date2020-01-07
dc.cm.id94562
dc.contributor.authorAsp, Juliapl
dc.contributor.authorSkov, Vibepl
dc.contributor.authorBellosillo, Beatrizpl
dc.contributor.authorKristensen, Thomaspl
dc.contributor.authorLippert, Ericpl
dc.contributor.authorDicker, Frankpl
dc.contributor.authorSchwarz, Jiripl
dc.contributor.authorWojtaszewska, Marzenapl
dc.contributor.authorAkiki, Susannapl
dc.contributor.authorAggerholm, Annipl
dc.contributor.authorAndersen, Morten Tolstruppl
dc.contributor.authorGirodon, Francoispl
dc.contributor.authorKjaer, Lassepl
dc.contributor.authorOppliger Leibundgut, Elisabethpl
dc.contributor.authorPancrazzi, Alessandropl
dc.contributor.authorVorland, Martapl
dc.contributor.authorAndrikovics, Hajnalkapl
dc.contributor.authorKralovics, Robertpl
dc.contributor.authorCassinat, Brunopl
dc.contributor.authorCoucelo, Margaridapl
dc.contributor.authorEftimov, Aleksandarpl
dc.contributor.authorHaslam, Karlpl
dc.contributor.authorKusec, Rajkopl
dc.contributor.authorLink-Lenczowska, Dorotapl
dc.contributor.authorLode, Laurencepl
dc.contributor.authorMatiakowska, Karolinapl
dc.contributor.authorNaguib, Dinapl
dc.contributor.authorNavaglia, Filippopl
dc.contributor.authorNovotny, Guy Waynepl
dc.contributor.authorPercy, Melanie J.pl
dc.contributor.authorSudarikov, Andreypl
dc.contributor.authorHermouet, Sylviepl
dc.contributor.authorPallisgaard, Nielspl
dc.date.accessioned2020-01-17T10:09:14Z
dc.date.available2020-01-17T10:09:14Z
dc.date.issued2019pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number5pl
dc.description.physical1111-1118pl
dc.description.points70pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume98pl
dc.identifier.doi10.1007/s00277-018-3570-8pl
dc.identifier.eissn1432-0584pl
dc.identifier.issn0939-5555pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/146317
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeinne
dc.subject.enJAK2 V617Fpl
dc.subject.enexternal quality assurancepl
dc.subject.enmyeloproliferative neoplasmspl
dc.subject.enquantitative PCRpl
dc.subtypeArticlepl
dc.titleInternational external quality assurance of "JAK2 V617F" quantificationpl
dc.title.journalAnnals of Hematologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T20:08:40Z
dc.abstract.enpl
External quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be more accurate than limits of the technology used, and it is essential to recognize factors causing substantial outlier results. The present study aimed to identify parameters of specific importance for JAK2 V617F quantification by quantitative PCR, using different starting materials, assays, and technical platforms. Sixteen samples were issued to participating laboratories in two EQA rounds. In the first round, 19 laboratories from 11 European countries analyzing JAK2 V617F as part of their routine diagnostics returned results from in-house assays. In the second round, 25 laboratories from 17 countries participated. Despite variations in starting material, assay set-up and instrumentation the laboratories were generally well aligned in the EQA program. However, EQA based on a single technology appears to be a valuable tool to achieve standardization of the quantification of JAK2 V617F allelic burden.
dc.affiliationpl
Wydział Lekarski
dc.cm.date
2020-01-07
dc.cm.id
94562
dc.contributor.authorpl
Asp, Julia
dc.contributor.authorpl
Skov, Vibe
dc.contributor.authorpl
Bellosillo, Beatriz
dc.contributor.authorpl
Kristensen, Thomas
dc.contributor.authorpl
Lippert, Eric
dc.contributor.authorpl
Dicker, Frank
dc.contributor.authorpl
Schwarz, Jiri
dc.contributor.authorpl
Wojtaszewska, Marzena
dc.contributor.authorpl
Akiki, Susanna
dc.contributor.authorpl
Aggerholm, Anni
dc.contributor.authorpl
Andersen, Morten Tolstrup
dc.contributor.authorpl
Girodon, Francois
dc.contributor.authorpl
Kjaer, Lasse
dc.contributor.authorpl
Oppliger Leibundgut, Elisabeth
dc.contributor.authorpl
Pancrazzi, Alessandro
dc.contributor.authorpl
Vorland, Marta
dc.contributor.authorpl
Andrikovics, Hajnalka
dc.contributor.authorpl
Kralovics, Robert
dc.contributor.authorpl
Cassinat, Bruno
dc.contributor.authorpl
Coucelo, Margarida
dc.contributor.authorpl
Eftimov, Aleksandar
dc.contributor.authorpl
Haslam, Karl
dc.contributor.authorpl
Kusec, Rajko
dc.contributor.authorpl
Link-Lenczowska, Dorota
dc.contributor.authorpl
Lode, Laurence
dc.contributor.authorpl
Matiakowska, Karolina
dc.contributor.authorpl
Naguib, Dina
dc.contributor.authorpl
Navaglia, Filippo
dc.contributor.authorpl
Novotny, Guy Wayne
dc.contributor.authorpl
Percy, Melanie J.
dc.contributor.authorpl
Sudarikov, Andrey
dc.contributor.authorpl
Hermouet, Sylvie
dc.contributor.authorpl
Pallisgaard, Niels
dc.date.accessioned
2020-01-17T10:09:14Z
dc.date.available
2020-01-17T10:09:14Z
dc.date.issuedpl
2019
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
5
dc.description.physicalpl
1111-1118
dc.description.pointspl
70
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
98
dc.identifier.doipl
10.1007/s00277-018-3570-8
dc.identifier.eissnpl
1432-0584
dc.identifier.issnpl
0939-5555
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/146317
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
inne
dc.subject.enpl
JAK2 V617F
dc.subject.enpl
external quality assurance
dc.subject.enpl
myeloproliferative neoplasms
dc.subject.enpl
quantitative PCR
dc.subtypepl
Article
dc.titlepl
International external quality assurance of "JAK2 V617F" quantification
dc.title.journalpl
Annals of Hematology
dc.typepl
JournalArticle
dspace.entity.type
Publication
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