International external quality assurance of "JAK2 V617F" quantification

doi:10.1007/s00277-018-3570-8

International external quality assurance of "JAK2 V617F" quantification

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International external quality assurance of "JAK2 V617F" quantification

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punktacja MEiN [2019]: 70

title:	International external quality assurance of "JAK2 V617F" quantification
author:	Asp Julia, Skov Vibe, Bellosillo Beatriz, Kristensen Thomas, Lippert Eric, Dicker Frank, Schwarz Jiri, Wojtaszewska Marzena, Akiki Susanna, Aggerholm Anni, Andersen Morten Tolstrup, Girodon Francois, Kjaer Lasse, Oppliger Leibundgut Elisabeth, Pancrazzi Alessandro, Vorland Marta, Andrikovics Hajnalka, Kralovics Robert, Cassinat Bruno, Coucelo Margarida, Eftimov Aleksandar, Haslam Karl, Kusec Rajko, Link-Lenczowska Dorota, Lode Laurence, Matiakowska Karolina, Naguib Dina, Navaglia Filippo, Novotny Guy Wayne, Percy Melanie J., Sudarikov Andrey, Hermouet Sylvie, Pallisgaard Niels
journal title:	Annals of Hematology
volume:	98
issue:	5
date of publication :	2019
pages:	1111-1118
ISSN:	0939-5555
eISSN:	1432-0584
DOI:	10.1007/s00277-018-3570-8
language:	English
journal language:	English
abstract in English:	External quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be more accurate than limits of the technology used, and it is essential to recognize factors causing substantial outlier results. The present study aimed to identify parameters of specific importance for JAK2 V617F quantification by quantitative PCR, using different starting materials, assays, and technical platforms. Sixteen samples were issued to participating laboratories in two EQA rounds. In the first round, 19 laboratories from 11 European countries analyzing JAK2 V617F as part of their routine diagnostics returned results from in-house assays. In the second round, 25 laboratories from 17 countries participated. Despite variations in starting material, assay set-up and instrumentation the laboratories were generally well aligned in the EQA program. However, EQA based on a single technology appears to be a valuable tool to achieve standardization of the quantification of JAK2 V617F allelic burden.
keywords in English:	JAK2 V617F, external quality assurance, myeloproliferative neoplasms, quantitative PCR
departmental parameterization:	70
affiliation:	Wydział Lekarski
type:	journal article
subtype:	academic paper