Predictors of neutrophil extracellular traps markers in type 2 diabetes mellitus : associations with a prothrombotic state and hypofibrinolysis

2019
journal article
article
61
cris.lastimport.wos2024-04-09T21:31:29Z
dc.abstract.enBackground Type 2 diabetes mellitus (T2DM) is associated with a hypercoagulable state and increased neutrophil extracellular traps formation (NETosis). We investigated predictors of NETosis and cell death markers in circulating blood and their association with a prothrombotic state in T2DM. Methods In a cross-sectional study involving 113 T2DM patients aged 63.7 ± 8.2 years, we investigated citrullinated histone H3 (H3Cit), cell-free deoxyribonucleic acid (cfDNA), myeloperoxidase, neutrophil elastase, and inflammation markers, along with thrombin generation (TG), plasma clot lysis time (CLT), clot permeability (Ks) and fibrinolysis inhibitors. Results On multivariate logistic regression analysis adjusted for age and gender, predictors of high H3Cit (≥ 7.36 ng/mL, upper quartile) were: glycated hemoglobin (HbA1c) ≥ 7.0% and interleukin-6. Interleukin-6 was also found to be a predictor of high cfDNA (≥ 2.84 µg/mL, upper quartile) along with glucose. Citrullinated histone H3 and cfDNA correlated positively with CLT and inversely with Ks, while TG associated solely with cfDNA. These associations were not seen with myeloperoxidase and neutrophil elastase. Patients with previous myocardial infarction (n = 21, 18.6%) had higher H3Cit (+108%, p < 0.001) and cfDNA (+45%, p = 0.022). On multivariable analysis adjusted for potential confounders, H3Cit and cfDNA, along with plasminogen activator inhibitor-1 and concomitant cardiovascular disease, were predictors of CLT. Citrullinated histone H3 alone was a predictor of Ks and only cfDNA was a predictor of peak thrombin generated. Conclusions In T2DM, NETosis detectable in circulating blood is associated with inflammatory state and a prothrombotic state, especially hypofibrinolysis.pl
dc.affiliationWydział Lekarski : Instytut Kardiologiipl
dc.affiliationWydział Lekarski : Klinika Chorób Metabolicznychpl
dc.cm.date2020-01-07
dc.cm.id94035
dc.contributor.authorBryk, Agata - 163294 pl
dc.contributor.authorPrior, Shannon M.pl
dc.contributor.authorPlens, Krzysztofpl
dc.contributor.authorKonieczyńska, Małgorzatapl
dc.contributor.authorHohendorff, Jerzy - 114804 pl
dc.contributor.authorMałecki, Maciej - 130840 pl
dc.contributor.authorButenas, Sauliuspl
dc.contributor.authorUndas, Anetta - 133708 pl
dc.date.accessioned2020-01-17T10:07:59Z
dc.date.available2020-01-17T10:07:59Z
dc.date.issued2019pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.points140pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume18pl
dc.identifier.articleid49pl
dc.identifier.doi10.1186/s12933-019-0850-0pl
dc.identifier.eissn1475-2840
dc.identifier.issn1475-2840pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/146027
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.entype 2 diabetespl
dc.subject.enfibrinolysispl
dc.subject.enneutrophil extracellular trapspl
dc.subject.enfibrin clotpl
dc.subject.encardiovascular diseasepl
dc.subtypeArticlepl
dc.titlePredictors of neutrophil extracellular traps markers in type 2 diabetes mellitus : associations with a prothrombotic state and hypofibrinolysispl
dc.title.journalCardiovascular Diabetologypl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T21:31:29Z
dc.abstract.enpl
Background Type 2 diabetes mellitus (T2DM) is associated with a hypercoagulable state and increased neutrophil extracellular traps formation (NETosis). We investigated predictors of NETosis and cell death markers in circulating blood and their association with a prothrombotic state in T2DM. Methods In a cross-sectional study involving 113 T2DM patients aged 63.7 ± 8.2 years, we investigated citrullinated histone H3 (H3Cit), cell-free deoxyribonucleic acid (cfDNA), myeloperoxidase, neutrophil elastase, and inflammation markers, along with thrombin generation (TG), plasma clot lysis time (CLT), clot permeability (Ks) and fibrinolysis inhibitors. Results On multivariate logistic regression analysis adjusted for age and gender, predictors of high H3Cit (≥ 7.36 ng/mL, upper quartile) were: glycated hemoglobin (HbA1c) ≥ 7.0% and interleukin-6. Interleukin-6 was also found to be a predictor of high cfDNA (≥ 2.84 µg/mL, upper quartile) along with glucose. Citrullinated histone H3 and cfDNA correlated positively with CLT and inversely with Ks, while TG associated solely with cfDNA. These associations were not seen with myeloperoxidase and neutrophil elastase. Patients with previous myocardial infarction (n = 21, 18.6%) had higher H3Cit (+108%, p < 0.001) and cfDNA (+45%, p = 0.022). On multivariable analysis adjusted for potential confounders, H3Cit and cfDNA, along with plasminogen activator inhibitor-1 and concomitant cardiovascular disease, were predictors of CLT. Citrullinated histone H3 alone was a predictor of Ks and only cfDNA was a predictor of peak thrombin generated. Conclusions In T2DM, NETosis detectable in circulating blood is associated with inflammatory state and a prothrombotic state, especially hypofibrinolysis.
dc.affiliationpl
Wydział Lekarski : Instytut Kardiologii
dc.affiliationpl
Wydział Lekarski : Klinika Chorób Metabolicznych
dc.cm.date
2020-01-07
dc.cm.id
94035
dc.contributor.authorpl
Bryk, Agata - 163294
dc.contributor.authorpl
Prior, Shannon M.
dc.contributor.authorpl
Plens, Krzysztof
dc.contributor.authorpl
Konieczyńska, Małgorzata
dc.contributor.authorpl
Hohendorff, Jerzy - 114804
dc.contributor.authorpl
Małecki, Maciej - 130840
dc.contributor.authorpl
Butenas, Saulius
dc.contributor.authorpl
Undas, Anetta - 133708
dc.date.accessioned
2020-01-17T10:07:59Z
dc.date.available
2020-01-17T10:07:59Z
dc.date.issuedpl
2019
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.pointspl
140
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
18
dc.identifier.articleidpl
49
dc.identifier.doipl
10.1186/s12933-019-0850-0
dc.identifier.eissn
1475-2840
dc.identifier.issnpl
1475-2840
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/146027
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
type 2 diabetes
dc.subject.enpl
fibrinolysis
dc.subject.enpl
neutrophil extracellular traps
dc.subject.enpl
fibrin clot
dc.subject.enpl
cardiovascular disease
dc.subtypepl
Article
dc.titlepl
Predictors of neutrophil extracellular traps markers in type 2 diabetes mellitus : associations with a prothrombotic state and hypofibrinolysis
dc.title.journalpl
Cardiovascular Diabetology
dc.typepl
JournalArticle
dspace.entity.type
Publication
Affiliations

* The migration of download and view statistics prior to the date of April 8, 2024 is in progress.