In vitro cytotoxicity testing of new generation oxazaphosphorines against human histiocytic lymphoma cells

In vitro cytotoxicity testing of new generation ...

Bibliographic description

punktacja MNiSW [2013 A]: 20

title:	In vitro cytotoxicity testing of new generation oxazaphosphorines against human histiocytic lymphoma cells
author:	Opydo-Chanek Małgorzata , Mazur Lidia , Stojak Marta
Journal title:	Indian Journal of Experimental Biology
volume:	51
issue:	8
date of publication:	2013
pages:	615-622
ISSN:	0019-5189
eISSN:	0975-1009
URL:	http://nopr.niscair.res.in/bitstream/123456789/20484/1/IJEB%2051%288%29%20615-622.pdf
date accessed:	2015-07-01
language:	English
Journal language:	English
abstract in english:	Oxazaphosphorines belong to a group of alkylating agents. Mafosfamide cyclohexylamine salt (D-17272), 4-hydro-peroxy-cyclophosphamide (D-18864) and glufosfamide (D-19575, β-D-glucose-isophosphoramide mustard) are new generation oxazaphosphorines. The objective of the present study was to compare the cytotoxic action of these oxazaphosphorine compounds against human histiocytic lymphoma U937 cells. The chemical structures of the oxazaphosphorines were responsible for the different responses of U937 cells. The cytotoxic effects of D-17272, D-18864, and D-19575 on U937 cells depended on the agent tested, its dose, and the time intervals after the oxazaphosphorine application. Among the oxazaphosphorine agents, D-18864 appeared to be the most cytotoxic, and D-19575 was characterized by the lowest cytotoxicity. The in vitro cytotoxic activities of the oxazaphosphorines were strongly associated with their cell death inducing potential.
keywords in english:	apoptosis and necrosis, cell viability, cytotoxicity, mitochondrial membrane potential, oxazaphosphorines, U937 cells, cell count
affiliation:	Wydział Biologii i Nauk o Ziemi : Instytut Zoologii
type:	journal article
subtype:	academic paper