Identification of CD34+/PGDFRΑ+ valve interstitial cells (vics) in human aortic valves : association of their abundance, morphology and spatial organization with early calcific remodeling

2020
journal article
article
cris.lastimport.wos2024-04-09T23:30:29Z
dc.abstract.enAortic valve interstitial cells (VICs) constitute a heterogeneous population involved in the maintenance of unique valvular architecture, ensuring proper hemodynamic function but also engaged in valve degeneration. Recently, cells similar to telocytes/interstitial Cajal-like cells described in various organs were found in heart valves. The aim of this study was to examine the density, distribution, and spatial organization of a VIC subset co-expressing CD34 and PDGFRα in normal aortic valves and to investigate if these cells are associated with the occurrence of early signs of valve calcific remodeling. We examined 28 human aortic valves obtained upon autopsy. General valve morphology and the early signs of degeneration were assessed histochemically. The studied VICs were identified by immunofluorescence (CD34, PDGFRα, vimentin), and their number in standardized parts and layers of the valves was evaluated. In order to show the complex three-dimensional structure of CD34+/PDGFRα+ VICs, whole-mount specimens were imaged by confocal microscopy, and subsequently rendered using the Imaris (Bitplane AG, Zürich, Switzerland) software. CD34+/PDGFRα+ VICs were found in all examined valves, showing significant differences in the number, distribution within valve tissue, spatial organization, and morphology (spherical/oval without projections; numerous short projections; long, branching, occasionally moniliform projections). Such a complex morphology was associated with the younger age of the subjects, and these VICs were more frequent in the spongiosa layer of the valve. Both the number and percentage of CD34+/PDGFRα+ VICs were inversely correlated with the age of the subjects. Valves with histochemical signs of early calcification contained a lower number of CD34+/PDGFRα+ cells. They were less numerous in proximal parts of the cusps, i.e., areas prone to calcification. The results suggest that normal aortic valves contain a subpopulation of CD34+/PDGFRα+ VICs, which might be involved in the maintenance of local microenvironment resisting to pathologic remodeling. Their reduced number in older age could limit the self-regenerative properties of the valve stroma.pl
dc.affiliationWydział Lekarski : Zakład Patofizjologiipl
dc.affiliationWydział Lekarski : Zakład Histologiipl
dc.affiliationWydział Lekarski : Zakład Anatomiipl
dc.cm.date2021-02-15
dc.cm.id100724
dc.contributor.authorLis, Grzegorz - 130689 pl
dc.contributor.authorDubrowski, Andrzej - 203368 pl
dc.contributor.authorLis, Maciejpl
dc.contributor.authorSolewski, Bernardpl
dc.contributor.authorWitkowska, Karolinapl
dc.contributor.authorAleksandrovych, Veronika - 254962 pl
dc.contributor.authorJasek-Gajda, Ewa - 159982 pl
dc.contributor.authorHołda, Mateusz - 203774 pl
dc.contributor.authorGil, Krzysztof - 129499 pl
dc.contributor.authorLitwin, Jan - 130699 pl
dc.date.accessioned2021-02-15T01:22:14Z
dc.date.available2021-02-15T01:22:14Z
dc.date.issued2020pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.number17pl
dc.description.points140
dc.description.versionostateczna wersja wydawcy
dc.description.volume21pl
dc.identifier.articleid6330pl
dc.identifier.doi10.3390/ijms21176330pl
dc.identifier.eissn1422-0067pl
dc.identifier.projectROD UJ / OPpl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/263949
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeotwarte czasopismo
dc.subject.envalve interstitial cellspl
dc.subject.encalcificationpl
dc.subject.enCD34pl
dc.subject.enPDGFRαpl
dc.subject.encalcific aortic valve diseasepl
dc.subtypeArticlepl
dc.titleIdentification of CD34+/PGDFRΑ+ valve interstitial cells (vics) in human aortic valves : association of their abundance, morphology and spatial organization with early calcific remodelingpl
dc.title.journalInternational Journal of Molecular Sciencespl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T23:30:29Z
dc.abstract.enpl
Aortic valve interstitial cells (VICs) constitute a heterogeneous population involved in the maintenance of unique valvular architecture, ensuring proper hemodynamic function but also engaged in valve degeneration. Recently, cells similar to telocytes/interstitial Cajal-like cells described in various organs were found in heart valves. The aim of this study was to examine the density, distribution, and spatial organization of a VIC subset co-expressing CD34 and PDGFRα in normal aortic valves and to investigate if these cells are associated with the occurrence of early signs of valve calcific remodeling. We examined 28 human aortic valves obtained upon autopsy. General valve morphology and the early signs of degeneration were assessed histochemically. The studied VICs were identified by immunofluorescence (CD34, PDGFRα, vimentin), and their number in standardized parts and layers of the valves was evaluated. In order to show the complex three-dimensional structure of CD34+/PDGFRα+ VICs, whole-mount specimens were imaged by confocal microscopy, and subsequently rendered using the Imaris (Bitplane AG, Zürich, Switzerland) software. CD34+/PDGFRα+ VICs were found in all examined valves, showing significant differences in the number, distribution within valve tissue, spatial organization, and morphology (spherical/oval without projections; numerous short projections; long, branching, occasionally moniliform projections). Such a complex morphology was associated with the younger age of the subjects, and these VICs were more frequent in the spongiosa layer of the valve. Both the number and percentage of CD34+/PDGFRα+ VICs were inversely correlated with the age of the subjects. Valves with histochemical signs of early calcification contained a lower number of CD34+/PDGFRα+ cells. They were less numerous in proximal parts of the cusps, i.e., areas prone to calcification. The results suggest that normal aortic valves contain a subpopulation of CD34+/PDGFRα+ VICs, which might be involved in the maintenance of local microenvironment resisting to pathologic remodeling. Their reduced number in older age could limit the self-regenerative properties of the valve stroma.
dc.affiliationpl
Wydział Lekarski : Zakład Patofizjologii
dc.affiliationpl
Wydział Lekarski : Zakład Histologii
dc.affiliationpl
Wydział Lekarski : Zakład Anatomii
dc.cm.date
2021-02-15
dc.cm.id
100724
dc.contributor.authorpl
Lis, Grzegorz - 130689
dc.contributor.authorpl
Dubrowski, Andrzej - 203368
dc.contributor.authorpl
Lis, Maciej
dc.contributor.authorpl
Solewski, Bernard
dc.contributor.authorpl
Witkowska, Karolina
dc.contributor.authorpl
Aleksandrovych, Veronika - 254962
dc.contributor.authorpl
Jasek-Gajda, Ewa - 159982
dc.contributor.authorpl
Hołda, Mateusz - 203774
dc.contributor.authorpl
Gil, Krzysztof - 129499
dc.contributor.authorpl
Litwin, Jan - 130699
dc.date.accessioned
2021-02-15T01:22:14Z
dc.date.available
2021-02-15T01:22:14Z
dc.date.issuedpl
2020
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.numberpl
17
dc.description.points
140
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
21
dc.identifier.articleidpl
6330
dc.identifier.doipl
10.3390/ijms21176330
dc.identifier.eissnpl
1422-0067
dc.identifier.projectpl
ROD UJ / OP
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/263949
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
otwarte czasopismo
dc.subject.enpl
valve interstitial cells
dc.subject.enpl
calcification
dc.subject.enpl
CD34
dc.subject.enpl
PDGFRα
dc.subject.enpl
calcific aortic valve disease
dc.subtypepl
Article
dc.titlepl
Identification of CD34+/PGDFRΑ+ valve interstitial cells (vics) in human aortic valves : association of their abundance, morphology and spatial organization with early calcific remodeling
dc.title.journalpl
International Journal of Molecular Sciences
dc.typepl
JournalArticle
dspace.entity.type
Publication

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