Identification of mitogen-activated protein kinase phosphatase-1 (MKP-1) protein partners using tandem affinity purification and mass spectrometry

2023
journal article
article
cris.lastimport.wos2024-04-09T22:21:19Z
dc.abstract.enBackground According to the World Health Organization Report, depressive disorders affect about 10% of the population. The molecular mechanism of the pathogenesis of depression is still not well understood. The new findings point to phosphatases as potential targets for effective depression therapy. The aim of the present work was the development of a method that would enable the identification of mitogen-activated protein kinase phosphatase-1 (MKP-1) protein partners using a proteomic approach. Methods The research was carried out using the PC12 cell line, often used as a model for neurobiological research. The use of the procedure for efficient purification of protein complexes—tandem affinity purification (TAP) will facilitate the identification of proteins interacting with MKP-1, a potential goal of effective antidepressant therapy. Results Identified proteins belong to various groups: cytoskeletal, ribosomal, nucleic acid binding, chaperones, and enzymes and may potentially be involved in the molecular mechanism of depression. Conclusions The presented protocol for the purification of protein complexes is universal and can be successfully used in different mammalian cell lines. Proteins identified in the present work have been reported in the literature concerning studies on depressive disorders, which speaks in favour of their role in depression.pl
dc.affiliationWydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznejpl
dc.contributor.authorFic, Ewelina - 127897 pl
dc.contributor.authorCieślik, Agatapl
dc.contributor.authorFigiel, Małgorzata - 106889 pl
dc.contributor.authorDziedzicka-Wasylewska, Marta - 127854 pl
dc.date.accessioned2023-03-31T17:11:02Z
dc.date.available2023-03-31T17:11:02Z
dc.date.issued2023pl
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalBibliogr. s. 480-481pl
dc.description.number2pl
dc.description.physical474-481pl
dc.description.versionostateczna wersja wydawcy
dc.description.volume75pl
dc.identifier.doi10.1007/s43440-023-00471-7pl
dc.identifier.eissn2299-5684pl
dc.identifier.issn1734-1140pl
dc.identifier.urihttps://ruj.uj.edu.pl/xmlui/handle/item/309701
dc.languageengpl
dc.language.containerengpl
dc.rightsUdzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa*
dc.rights.licenceCC-BY
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/legalcode.pl*
dc.share.typeinne
dc.subject.endepressionpl
dc.subject.enafnity chromatographypl
dc.subject.entagspl
dc.subject.eninteractomicspl
dc.subject.enPC12 cell linepl
dc.subtypeArticlepl
dc.titleIdentification of mitogen-activated protein kinase phosphatase-1 (MKP-1) protein partners using tandem affinity purification and mass spectrometrypl
dc.title.journalPharmacological Reportspl
dc.typeJournalArticlepl
dspace.entity.typePublication
cris.lastimport.wos
2024-04-09T22:21:19Z
dc.abstract.enpl
Background According to the World Health Organization Report, depressive disorders affect about 10% of the population. The molecular mechanism of the pathogenesis of depression is still not well understood. The new findings point to phosphatases as potential targets for effective depression therapy. The aim of the present work was the development of a method that would enable the identification of mitogen-activated protein kinase phosphatase-1 (MKP-1) protein partners using a proteomic approach. Methods The research was carried out using the PC12 cell line, often used as a model for neurobiological research. The use of the procedure for efficient purification of protein complexes—tandem affinity purification (TAP) will facilitate the identification of proteins interacting with MKP-1, a potential goal of effective antidepressant therapy. Results Identified proteins belong to various groups: cytoskeletal, ribosomal, nucleic acid binding, chaperones, and enzymes and may potentially be involved in the molecular mechanism of depression. Conclusions The presented protocol for the purification of protein complexes is universal and can be successfully used in different mammalian cell lines. Proteins identified in the present work have been reported in the literature concerning studies on depressive disorders, which speaks in favour of their role in depression.
dc.affiliationpl
Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznej
dc.contributor.authorpl
Fic, Ewelina - 127897
dc.contributor.authorpl
Cieślik, Agata
dc.contributor.authorpl
Figiel, Małgorzata - 106889
dc.contributor.authorpl
Dziedzicka-Wasylewska, Marta - 127854
dc.date.accessioned
2023-03-31T17:11:02Z
dc.date.available
2023-03-31T17:11:02Z
dc.date.issuedpl
2023
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additionalpl
Bibliogr. s. 480-481
dc.description.numberpl
2
dc.description.physicalpl
474-481
dc.description.version
ostateczna wersja wydawcy
dc.description.volumepl
75
dc.identifier.doipl
10.1007/s43440-023-00471-7
dc.identifier.eissnpl
2299-5684
dc.identifier.issnpl
1734-1140
dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/309701
dc.languagepl
eng
dc.language.containerpl
eng
dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa
dc.rights.licence
CC-BY
dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl
dc.share.type
inne
dc.subject.enpl
depression
dc.subject.enpl
afnity chromatography
dc.subject.enpl
tags
dc.subject.enpl
interactomics
dc.subject.enpl
PC12 cell line
dc.subtypepl
Article
dc.titlepl
Identification of mitogen-activated protein kinase phosphatase-1 (MKP-1) protein partners using tandem affinity purification and mass spectrometry
dc.title.journalpl
Pharmacological Reports
dc.typepl
JournalArticle
dspace.entity.type
Publication

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