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Host-derived protease promotes aggregation of Staphylococcus aureus by cleaving the surface protein SasG
Staphylococcus aureus
MRSA
SasG
lung infection
aggregation
Staphylococcus aureus is one of the leading causes of hospital-acquired infections, many of which begin following attachment and accumulation on indwelling medical devices or diseased tissue. These infections are often linked to the establishment of biofilms, but another often overlooked key characteristic allowing S. aureus to establish persistent infection is the formation of planktonic aggregates. Such aggregates are physiologically similar to biofilms and protect pathogens from innate immune clearance and increase antibiotic tolerance. The cell-wall-associated protein SasG has been implicated in biofilm formation via mechanisms of intercellular aggregation but the mechanism in the context of disease is largely unknown. We have previously shown that the expression of cell-wall-anchored proteins involved in biofilm formation is controlled by the ArlRS-MgrA regulatory cascade. In this work, we demonstrate that the ArlRS two-component system controls aggregation, by repressing the expression of sasG by activation of the global regulator MgrA. We also demonstrate that SasG must be proteolytically processed by a non-staphylococcal protease to induce aggregation and that strains expressing functional full-length sasG aggregate significantly upon proteolysis by a mucosal-derived host protease found in human saliva. We used fractionation and N-terminal sequencing to demonstrate that human trypsin within saliva cleaves within the A domain of SasG to expose the B domain and induce aggregation. Finally, we demonstrated that SasG is involved in virulence during mouse lung infection. Together, our data point to SasG, its processing by host proteases, and SasG-driven aggregation as important elements of S. aureus adaptation to the host environment.
cris.lastimport.scopus | 2024-04-29T01:27:56Z | |
dc.abstract.en | Staphylococcus aureus is one of the leading causes of hospital-acquired infections, many of which begin following attachment and accumulation on indwelling medical devices or diseased tissue. These infections are often linked to the establishment of biofilms, but another often overlooked key characteristic allowing S. aureus to establish persistent infection is the formation of planktonic aggregates. Such aggregates are physiologically similar to biofilms and protect pathogens from innate immune clearance and increase antibiotic tolerance. The cell-wall-associated protein SasG has been implicated in biofilm formation via mechanisms of intercellular aggregation but the mechanism in the context of disease is largely unknown. We have previously shown that the expression of cell-wall-anchored proteins involved in biofilm formation is controlled by the ArlRS-MgrA regulatory cascade. In this work, we demonstrate that the ArlRS two-component system controls aggregation, by repressing the expression of sasG by activation of the global regulator MgrA. We also demonstrate that SasG must be proteolytically processed by a non-staphylococcal protease to induce aggregation and that strains expressing functional full-length sasG aggregate significantly upon proteolysis by a mucosal-derived host protease found in human saliva. We used fractionation and N-terminal sequencing to demonstrate that human trypsin within saliva cleaves within the A domain of SasG to expose the B domain and induce aggregation. Finally, we demonstrated that SasG is involved in virulence during mouse lung infection. Together, our data point to SasG, its processing by host proteases, and SasG-driven aggregation as important elements of S. aureus adaptation to the host environment. | pl |
dc.affiliation | Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Mikrobiologii | pl |
dc.contributor.author | Crosby, Heidi A. | |
dc.contributor.author | Keim, Klara | |
dc.contributor.author | Kwieciński, Jakub - 103976 | |
dc.contributor.author | Langouët-Astrié, Christophe J. | |
dc.contributor.author | Oshima, Kaori | |
dc.contributor.author | LaRivière, Wells B. | |
dc.contributor.author | Schmidt, Eric P. | |
dc.contributor.author | Horswill, Alexander R. | |
dc.date.accessioned | 2024-04-24T09:38:18Z | |
dc.date.available | 2024-04-24T09:38:18Z | |
dc.date.issued | 2024 | pl |
dc.date.openaccess | 0 | |
dc.description.accesstime | w momencie opublikowania | |
dc.description.additional | Bibliogr. Jakub Kwieciński podpisany: Jakub M. Kwieciński | |
dc.description.number | 4 | |
dc.description.version | ostateczna wersja wydawcy | |
dc.description.volume | 15 | |
dc.identifier.articleid | e03483-23 | pl |
dc.identifier.doi | 10.1128/mbio.03483-23 | pl |
dc.identifier.eissn | 2150-7511 | pl |
dc.identifier.issn | 2161-2129 | pl |
dc.identifier.uri | https://ruj.uj.edu.pl/handle/item/331431 | |
dc.language | eng | pl |
dc.language.container | eng | pl |
dc.rights | Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa | |
dc.rights.licence | CC-BY | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/legalcode.pl | |
dc.share.type | otwarte czasopismo | |
dc.subject.en | Staphylococcus aureus | pl |
dc.subject.en | MRSA | pl |
dc.subject.en | SasG | pl |
dc.subject.en | lung infection | pl |
dc.subject.en | aggregation | pl |
dc.subtype | Article | pl |
dc.title | Host-derived protease promotes aggregation of Staphylococcus aureus by cleaving the surface protein SasG | |
dc.title.journal | mBio | pl |
dc.type | JournalArticle | pl |
dspace.entity.type | Publication |
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Except as otherwise noted, this item is licensed under the Attribution 4.0 International licence