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Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution : ROA and CPL study

2025
journal article
article
10.1039/D5CC00971E
Journal
Chem. Commun.
200
Author
Halat Monika

Klimek-Chodacka Magdalena

Domagała Agnieszka

Zając Grzegorz

Oleszkiewicz Tomasz
Show more
Pages
[1-4]
ISSN
1359-73451364-548X
URL
https://pubs.rsc.org/en/content/articlelanding/2025/cc/d5cc00971e
Access date
2025-04-24
Remarks

Online First 2025-04-15

Language
English
Journal language
English
Abstract in English

We present a new non-destructive approach to detect CRISPR-Cas biomolecules and distinguish active from inactive forms using Raman optical activity (ROA) and sensitive Eu-probes inducing circularly polarized luminescence (CPL).

Affiliation
Pion Prorektora ds. badań naukowych : Jagiellońskie Centrum Rozwoju Leków
dc.abstract.enWe present a new non-destructive approach to detect CRISPR-Cas biomolecules and distinguish active from inactive forms using Raman optical activity (ROA) and sensitive Eu-probes inducing circularly polarized luminescence (CPL).
dc.affiliationPion Prorektora ds. badań naukowych : Jagiellońskie Centrum Rozwoju Leków
dc.contributor.authorHalat, Monika - 189253
dc.contributor.authorKlimek-Chodacka, Magdalena
dc.contributor.authorDomagała, Agnieszka - 388803
dc.contributor.authorZając, Grzegorz - 177939
dc.contributor.authorOleszkiewicz, Tomasz - 511524
dc.contributor.authorKapitán, Josef
dc.contributor.authorBarański, Rafał
dc.date.accession2025-04-24
dc.date.accessioned2025-04-24T13:13:30Z
dc.date.available2025-04-24T13:13:30Z
dc.date.createdat2025-04-22T14:29:27Zen
dc.date.issued2025
dc.date.openaccess0
dc.description.accesstimew momencie opublikowania
dc.description.additionalOnline First 2025-04-15
dc.description.physical[1-4]
dc.description.versionostateczna wersja wydawcy
dc.identifier.doi10.1039/D5CC00971E
dc.identifier.issn1359-7345
dc.identifier.issn1364-548X
dc.identifier.urihttps://ruj.uj.edu.pl/handle/item/551803
dc.identifier.weblinkhttps://pubs.rsc.org/en/content/articlelanding/2025/cc/d5cc00971e
dc.languageeng
dc.language.containereng
dc.rightsDodaję tylko opis bibliograficzny
dc.rights.licenceCC-BY
dc.share.typeotwarte czasopismo
dc.sourceChem. Commun.
dc.subtypeArticle
dc.titleChiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution : ROA and CPL study
dc.title.journalChem. Commun.
dc.typeJournalArticle
dspace.entity.typePublicationen
dc.abstract.en
We present a new non-destructive approach to detect CRISPR-Cas biomolecules and distinguish active from inactive forms using Raman optical activity (ROA) and sensitive Eu-probes inducing circularly polarized luminescence (CPL).
dc.affiliation
Pion Prorektora ds. badań naukowych : Jagiellońskie Centrum Rozwoju Leków
dc.contributor.author
Halat, Monika - 189253
dc.contributor.author
Klimek-Chodacka, Magdalena
dc.contributor.author
Domagała, Agnieszka - 388803
dc.contributor.author
Zając, Grzegorz - 177939
dc.contributor.author
Oleszkiewicz, Tomasz - 511524
dc.contributor.author
Kapitán, Josef
dc.contributor.author
Barański, Rafał
dc.date.accession
2025-04-24
dc.date.accessioned
2025-04-24T13:13:30Z
dc.date.available
2025-04-24T13:13:30Z
dc.date.createdaten
2025-04-22T14:29:27Z
dc.date.issued
2025
dc.date.openaccess
0
dc.description.accesstime
w momencie opublikowania
dc.description.additional
Online First 2025-04-15
dc.description.physical
[1-4]
dc.description.version
ostateczna wersja wydawcy
dc.identifier.doi
10.1039/D5CC00971E
dc.identifier.issn
1359-7345
dc.identifier.issn
1364-548X
dc.identifier.uri
https://ruj.uj.edu.pl/handle/item/551803
dc.identifier.weblink
https://pubs.rsc.org/en/content/articlelanding/2025/cc/d5cc00971e
dc.language
eng
dc.language.container
eng
dc.rights
Dodaję tylko opis bibliograficzny
dc.rights.licence
CC-BY
dc.share.type
otwarte czasopismo
dc.source
Chem. Commun.
dc.subtype
Article
dc.title
Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution : ROA and CPL study
dc.title.journal
Chem. Commun.
dc.type
JournalArticle
dspace.entity.typeen
Publication
Affiliations
No affiliation
Halat, Monika
Klimek-Chodacka, Magdalena
Oleszkiewicz, Tomasz
Kapitán, Josef
Barański, Rafał
Szkoła Doktorska Nauk Ścisłych i Przyrodniczych
Domagała, Agnieszka
biological sciences
Jagiellońskie Centrum Rozwoju Leków
Zając, Grzegorz
chemical sciences

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