Simple view
Full metadata view
Authors
Statistics
Study of oxidation of ciprofloxacin and pefloxacin by ACVA : identification of degradation products by mass spectrometry and bioautographic evaluation of antibacterial activity
Journal
Processes
70
Author
Volume
10
Number
5
Article ID
1022
ISSN
2227-9717
eISSN
2227-9717
Keywords in English
ciprofloxacin
pefloxacin
RP-HPLC-DAD method
oxidation studies
ACVA
degradation products
mass spectrometry
direct bioautography
Access date
2022-05-24
Language
English
Journal language
English
Abstract in English
The new RP-HPLC-DAD method for the determination of ciprofloxacin and pefloxacin, next to their degradation products after the oxidation reaction with 4,4′-azobis(4-cyanopentanoic acid) (ACVA) was developed. The method was validated according to the guidelines of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) and meets the acceptance criteria. The experimental data indicate that the course of the oxidation process depends on the type of fluoroquinolone (FQ), the incubation time and temperature. The performed kinetic evaluation allowed us to state that the oxidation of FQs proceeds according to the second-order kinetics. The degradation products of the FQs were identified using the UHPLC-MS/MS method and their structures were proposed. The results obtained by the TLC-direct bioautography technique allowed us to state that the main ciprofloxacin and pefloxacin oxidation products probably retained antibacterial activity against Escherichia coli.
Affiliation
Wydział Farmaceutyczny : Zakład Chemii LekówWydział Farmaceutyczny : Zakład Chemii NieorganicznejWydział Farmaceutyczny : Zakład Chemii Analitycznej
Scopus© citations
7
| dc.abstract.en | The new RP-HPLC-DAD method for the determination of ciprofloxacin and pefloxacin, next to their degradation products after the oxidation reaction with 4,4′-azobis(4-cyanopentanoic acid) (ACVA) was developed. The method was validated according to the guidelines of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) and meets the acceptance criteria. The experimental data indicate that the course of the oxidation process depends on the type of fluoroquinolone (FQ), the incubation time and temperature. The performed kinetic evaluation allowed us to state that the oxidation of FQs proceeds according to the second-order kinetics. The degradation products of the FQs were identified using the UHPLC-MS/MS method and their structures were proposed. The results obtained by the TLC-direct bioautography technique allowed us to state that the main ciprofloxacin and pefloxacin oxidation products probably retained antibacterial activity against Escherichia coli. | |
| dc.affiliation | Wydział Farmaceutyczny : Zakład Chemii Leków | pl |
| dc.affiliation | Wydział Farmaceutyczny : Zakład Chemii Nieorganicznej | pl |
| dc.affiliation | Wydział Farmaceutyczny : Zakład Chemii Analitycznej | pl |
| dc.cm.date | 2022-05-25T03:11:43Z | |
| dc.cm.id | 108146 | pl |
| dc.cm.idOmega | UJCM7a5ae903612b4236b1df199c8c9dc918 | pl |
| dc.contributor.author | Żuromska-Witek, Barbara - 200539 | pl |
| dc.contributor.author | Żmudzki, Paweł - 214411 | pl |
| dc.contributor.author | Szlósarczyk, Marek - 133590 | pl |
| dc.contributor.author | Abram, Michał - 178828 | pl |
| dc.contributor.author | Maślanka, Anna - 130890 | pl |
| dc.contributor.author | Hubicka, Urszula - 129734 | pl |
| dc.date.accession | 2022-05-24 | pl |
| dc.date.accessioned | 2022-05-25T03:11:43Z | |
| dc.date.available | 2022-05-25T03:11:43Z | |
| dc.date.issued | 2022 | pl |
| dc.date.openaccess | 0 | |
| dc.description.accesstime | w momencie opublikowania | |
| dc.description.number | 5 | pl |
| dc.description.version | ostateczna wersja wydawcy | |
| dc.description.volume | 10 | pl |
| dc.identifier.articleid | 1022 | pl |
| dc.identifier.doi | 10.3390/pr10051022 | pl |
| dc.identifier.eissn | 2227-9717 | pl |
| dc.identifier.issn | 2227-9717 | pl |
| dc.identifier.uri | https://ruj.uj.edu.pl/xmlui/handle/item/292265 | |
| dc.identifier.weblink | https://www.mdpi.com/2227-9717/10/5/1022 | pl |
| dc.language | eng | pl |
| dc.language.container | eng | pl |
| dc.pbn.affiliation | Dziedzina nauk medycznych i nauk o zdrowiu : nauki farmaceutyczne | |
| dc.rights | Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa | |
| dc.rights.licence | CC-BY | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/legalcode.pl | |
| dc.share.type | Otwarte czasopismo | |
| dc.subject.en | ciprofloxacin | |
| dc.subject.en | pefloxacin | |
| dc.subject.en | RP-HPLC-DAD method | |
| dc.subject.en | oxidation studies | |
| dc.subject.en | ACVA | |
| dc.subject.en | degradation products | |
| dc.subject.en | mass spectrometry | |
| dc.subject.en | direct bioautography | |
| dc.subtype | Article | pl |
| dc.title | Study of oxidation of ciprofloxacin and pefloxacin by ACVA : identification of degradation products by mass spectrometry and bioautographic evaluation of antibacterial activity | pl |
| dc.title.journal | Processes | pl |
| dc.type | JournalArticle | pl |
| dspace.entity.type | Publication |
dc.abstract.en
The new RP-HPLC-DAD method for the determination of ciprofloxacin and pefloxacin, next to their degradation products after the oxidation reaction with 4,4′-azobis(4-cyanopentanoic acid) (ACVA) was developed. The method was validated according to the guidelines of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) and meets the acceptance criteria. The experimental data indicate that the course of the oxidation process depends on the type of fluoroquinolone (FQ), the incubation time and temperature. The performed kinetic evaluation allowed us to state that the oxidation of FQs proceeds according to the second-order kinetics. The degradation products of the FQs were identified using the UHPLC-MS/MS method and their structures were proposed. The results obtained by the TLC-direct bioautography technique allowed us to state that the main ciprofloxacin and pefloxacin oxidation products probably retained antibacterial activity against Escherichia coli. dc.affiliationpl
Wydział Farmaceutyczny : Zakład Chemii Leków dc.affiliationpl
Wydział Farmaceutyczny : Zakład Chemii Nieorganicznej dc.affiliationpl
Wydział Farmaceutyczny : Zakład Chemii Analitycznej dc.cm.date
2022-05-25T03:11:43Z dc.cm.idpl
108146 dc.cm.idOmegapl
UJCM7a5ae903612b4236b1df199c8c9dc918 dc.contributor.authorpl
Żuromska-Witek, Barbara - 200539 dc.contributor.authorpl
Żmudzki, Paweł - 214411 dc.contributor.authorpl
Szlósarczyk, Marek - 133590 dc.contributor.authorpl
Abram, Michał - 178828 dc.contributor.authorpl
Maślanka, Anna - 130890 dc.contributor.authorpl
Hubicka, Urszula - 129734 dc.date.accessionpl
2022-05-24 dc.date.accessioned
2022-05-25T03:11:43Z dc.date.available
2022-05-25T03:11:43Z dc.date.issuedpl
2022 dc.date.openaccess
0 dc.description.accesstime
w momencie opublikowania dc.description.numberpl
5 dc.description.version
ostateczna wersja wydawcy dc.description.volumepl
10 dc.identifier.articleidpl
1022 dc.identifier.doipl
10.3390/pr10051022 dc.identifier.eissnpl
2227-9717 dc.identifier.issnpl
2227-9717 dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/292265 dc.identifier.weblinkpl
https://www.mdpi.com/2227-9717/10/5/1022 dc.languagepl
eng dc.language.containerpl
eng dc.pbn.affiliation
Dziedzina nauk medycznych i nauk o zdrowiu : nauki farmaceutyczne dc.rights
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa dc.rights.licence
CC-BY dc.rights.uri
http://creativecommons.org/licenses/by/4.0/legalcode.pl dc.share.type
Otwarte czasopismo dc.subject.en
ciprofloxacin dc.subject.en
pefloxacin dc.subject.en
RP-HPLC-DAD method dc.subject.en
oxidation studies dc.subject.en
ACVA dc.subject.en
degradation products dc.subject.en
mass spectrometry dc.subject.en
direct bioautography dc.subtypepl
Article dc.titlepl
Study of oxidation of ciprofloxacin and pefloxacin by ACVA : identification of degradation products by mass spectrometry and bioautographic evaluation of antibacterial activity dc.title.journalpl
Processes dc.typepl
JournalArticle dspace.entity.type
Publication Affiliations
Wydział Farmaceutyczny
Żuromska-Witek, Barbara
Żmudzki, Paweł
Szlósarczyk, Marek
Abram, Michał
Maślanka, Anna
Hubicka, Urszula
* The migration of download and view statistics prior to the date of April 8, 2024 is in progress.
Views
18
Views per month
Views per city
Ashburn
3
Dublin
2
Krakow
2
Wroclaw
2
Istanbul
1
Krasnystaw
1
Lappeenranta
1
Downloads
zuromska-witek_et-al_study_of_oxidation_of_ciprofloxacin_and_pefloxacin_2022.pdf
12
Open Access
No Thumbnail Available
