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Lipid content in pig blastocysts cultured in the presence or absence of protein and vitamin E or phenazine ethosulfate
Journal
Folia Biologica
Author
Romek Marek
Gajda Barbara
Krzysztofowicz Ewa
Kępczyński Mariusz
Smorąg Zdzisław
Volume
59
Number
1-2
Pages
45-52
ISSN
0015-5497
eISSN
1734-9168
Keywords in English
pig embryo
lipid
vitamin E
PES
FCS
BSA
Access date
2018-11-21
Remarks
Bibliogr. s. 52.
Language
English
Journal language
English
Abstract in English
In the present study, total lipid content and content of triglycerides, phospholipids and cholesterol were determined in pig blastocysts cultured in medium without protein, supplemented with bovine serum albumin (BSA), with fetal calf serum (FCS), vitamin E or phenazine ethosulfate (PES). In comparison to blastocysts cultured in NCSU-23 with BSA, we observed a decrease of the total lipid content in PES-treated embryos. Triglyceride content in FCS-, vitamin E- and PES-treated embryos as well as in blastocysts cultured without protein was 81.9%, 70.2%, 57.2% and 74.8% of that found in the blastocysts cultured in NCSU-23 with BSA, respectively. Nevertheless the content of phospholipids remained unchanged. This decrease of triglyceride content in the porcine blastocyst after in vitro culture may be explained by altered lipid metabolism in embryos.
Affiliation
Wydział Chemii : Zakład Chemii Fizycznej i ElektrochemiiWydział Biologii i Nauk o Ziemi : Instytut Zoologii
Scopus© citations
7
| cris.lastimport.wos | 2024-04-09T18:33:49Z | |
| dc.abstract.en | In the present study, total lipid content and content of triglycerides, phospholipids and cholesterol were determined in pig blastocysts cultured in medium without protein, supplemented with bovine serum albumin (BSA), with fetal calf serum (FCS), vitamin E or phenazine ethosulfate (PES). In comparison to blastocysts cultured in NCSU-23 with BSA, we observed a decrease of the total lipid content in PES-treated embryos. Triglyceride content in FCS-, vitamin E- and PES-treated embryos as well as in blastocysts cultured without protein was 81.9%, 70.2%, 57.2% and 74.8% of that found in the blastocysts cultured in NCSU-23 with BSA, respectively. Nevertheless the content of phospholipids remained unchanged. This decrease of triglyceride content in the porcine blastocyst after in vitro culture may be explained by altered lipid metabolism in embryos. | pl |
| dc.affiliation | Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii | pl |
| dc.affiliation | Wydział Biologii i Nauk o Ziemi : Instytut Zoologii | pl |
| dc.contributor.author | Romek, Marek - 131692 | pl |
| dc.contributor.author | Gajda, Barbara | pl |
| dc.contributor.author | Krzysztofowicz, Ewa | pl |
| dc.contributor.author | Kępczyński, Mariusz - 128750 | pl |
| dc.contributor.author | Smorąg, Zdzisław | pl |
| dc.date.accession | 2018-11-21 | pl |
| dc.date.accessioned | 2018-11-21T14:54:35Z | |
| dc.date.available | 2018-11-21T14:54:35Z | |
| dc.date.issued | 2011 | pl |
| dc.date.openaccess | 0 | |
| dc.description.accesstime | w momencie opublikowania | |
| dc.description.additional | Bibliogr. s. 52. | pl |
| dc.description.number | 1-2 | pl |
| dc.description.physical | 45-52 | pl |
| dc.description.version | ostateczna wersja wydawcy | |
| dc.description.volume | 59 | pl |
| dc.identifier.doi | 10.3409/fb59_1-2.45-52 | pl |
| dc.identifier.eissn | 1734-9168 | pl |
| dc.identifier.issn | 0015-5497 | pl |
| dc.identifier.project | ROD UJ / OP | pl |
| dc.identifier.uri | https://ruj.uj.edu.pl/xmlui/handle/item/61172 | |
| dc.identifier.weblink | http://www.isez.pan.krakow.pl/journals/folia/pdf/59(1-2)/59(1-2)_09.pdf | pl |
| dc.language | eng | pl |
| dc.language.container | eng | pl |
| dc.rights | Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa | * |
| dc.rights.licence | CC-BY | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/legalcode.pl | * |
| dc.share.type | otwarte czasopismo | |
| dc.source.integrator | false | |
| dc.subject.en | pig embryo | pl |
| dc.subject.en | lipid | pl |
| dc.subject.en | vitamin E | pl |
| dc.subject.en | PES | pl |
| dc.subject.en | FCS | pl |
| dc.subject.en | BSA | pl |
| dc.subtype | Article | pl |
| dc.title | Lipid content in pig blastocysts cultured in the presence or absence of protein and vitamin E or phenazine ethosulfate | pl |
| dc.title.journal | Folia Biologica | pl |
| dc.type | JournalArticle | pl |
| dspace.entity.type | Publication |
cris.lastimport.wos
2024-04-09T18:33:49Z dc.abstract.enpl
In the present study, total lipid content and content of triglycerides, phospholipids and
cholesterol were determined in pig blastocysts cultured in medium without protein,
supplemented with bovine serum albumin (BSA), with fetal calf serum (FCS), vitamin E or
phenazine ethosulfate (PES). In comparison to blastocysts cultured in NCSU-23 with BSA,
we observed a decrease of the total lipid content in PES-treated embryos. Triglyceride
content in FCS-, vitamin E- and PES-treated embryos as well as in blastocysts cultured
without protein was 81.9%, 70.2%, 57.2% and 74.8% of that found in the blastocysts cultured
in NCSU-23 with BSA, respectively. Nevertheless the content of phospholipids remained
unchanged. This decrease of triglyceride content in the porcine blastocyst after in vitro
culture may be explained by altered lipid metabolism in embryos. dc.affiliationpl
Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii dc.affiliationpl
Wydział Biologii i Nauk o Ziemi : Instytut Zoologii dc.contributor.authorpl
Romek, Marek - 131692 dc.contributor.authorpl
Gajda, Barbara dc.contributor.authorpl
Krzysztofowicz, Ewa dc.contributor.authorpl
Kępczyński, Mariusz - 128750 dc.contributor.authorpl
Smorąg, Zdzisław dc.date.accessionpl
2018-11-21 dc.date.accessioned
2018-11-21T14:54:35Z dc.date.available
2018-11-21T14:54:35Z dc.date.issuedpl
2011 dc.date.openaccess
0 dc.description.accesstime
w momencie opublikowania dc.description.additionalpl
Bibliogr. s. 52. dc.description.numberpl
1-2 dc.description.physicalpl
45-52 dc.description.version
ostateczna wersja wydawcy dc.description.volumepl
59 dc.identifier.doipl
10.3409/fb59_1-2.45-52 dc.identifier.eissnpl
1734-9168 dc.identifier.issnpl
0015-5497 dc.identifier.projectpl
ROD UJ / OP dc.identifier.uri
https://ruj.uj.edu.pl/xmlui/handle/item/61172 dc.identifier.weblinkpl
http://www.isez.pan.krakow.pl/journals/folia/pdf/59(1-2)/59(1-2)_09.pdf dc.languagepl
eng dc.language.containerpl
eng dc.rights*
Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa dc.rights.licence
CC-BY dc.rights.uri*
http://creativecommons.org/licenses/by/4.0/legalcode.pl dc.share.type
otwarte czasopismo dc.source.integrator
false dc.subject.enpl
pig embryo dc.subject.enpl
lipid dc.subject.enpl
vitamin E dc.subject.enpl
PES dc.subject.enpl
FCS dc.subject.enpl
BSA dc.subtypepl
Article dc.titlepl
Lipid content in pig blastocysts cultured in the presence or absence of protein and vitamin E or phenazine ethosulfate dc.title.journalpl
Folia Biologica dc.typepl
JournalArticle dspace.entity.type
Publication Affiliations
No affiliation
Romek, Marek
Gajda, Barbara
Krzysztofowicz, Ewa
Kępczyński, Mariusz
Smorąg, Zdzisław
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