The launch is considered the most stressful rocket flight stage due to the hypergravity occurrences. The possibility of using honey bees (Apis mellifera) as the extraterrestrial pollinator depends on their ability to reproduce correctly after experiencing hypergravity. The described study aims to verify the impact of a launching rocket’s acceleration on honey bee queen’s egg-laying behavior. Four artificially inseminated A. mellifera carnica queens were placed in the Human Training Centrifuge and given to the acceleration pattern of the launching Soyuz rocket. Next, the data on the number of food stores, eggs, larvae, and worker and drone pupae were collected from the test and control hives using the modified Liebefeld method. The pilot study results imply that accelerated queen’s egg-laying behavior may change twofold: limiting or maximizing the number of laid eggs, with the control queen egg-laying rate remaining stable for all samples. The number of drone pupae is greater for the test sample colonies, with its earlier appearance in the hive. No impact on overwintering success was observed. Authors indicate limitations of the results and a need to continue the study to verify the occurrence of anomalies potentially related to the examined factor.