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Agnieszka Moos - Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Jagiellonian University.
Na publikacji autorka Moos-Matysik Agnieszka podpisana jako Moos Agnieszka.
język:
angielski
język czasopisma:
angielski
abstrakt w j. angielskim:
The
aim
of
this
study
was
to
apply
microextraction
by
packed
sorbent
(MEPS)
to
the
isolation
of
six
tricyclic
antidepressants
(TCADs):
nordoxepin,
doxepin,
desipramine,
nortriptyline,
imipramine,
and
amitripty-
line
from
human
oral
fluid.
Samples
were
collected
from
healthy
volunteers
via
free
spillage
from
the
oral
cavity
to
disposable
test
tubes.
A
method
of
oral
fluid
sample
pretreatment
was
developed
and
optimized
in
terms
of
suitability
for
MEPS
extraction
and
removing
of
interfering
agents
(protein,
food
debris,
or
air
bubbles).
Moreover,
it
was
short
and
simple
to
perform
with
limited
sample
consumption
(150
L).
Extracts
were
analysed
by
UHPLC–MS.
The
MEPS/UHPLC–MS
method
was
validated
at
three
concentration
levels
(2.00,
4.00
and
8.00
ng/mL)
of
all
analytes
in
the
range
1.25–10.0
ng/mL.
The
follow-
ing
parameters
were
determined:
limit
of
detection,
limit
of
quantification,
precision,
and
accuracy.
For
all
tested
concentration
levels,
the
intra-
and
inter-day
repeatability
did
not
exceeded
8.1%
and
12.2%,
respectively.
Gained
LOQ
value,
0.50
ng/mL,
made
the
MEPS/UHPLC–MS
method
to
be
a
useful
tool
in
clinical
and
forensic
laboratories,
which
was
demonstrated
on
the
basis
of
analysis
of
real
samples.