Endothelium in spots : high-content imaging of lipid rafts clusters in db/db mice

doi:10.1371/journal.pone.0106065

Endothelium in spots : high-content imaging of lipid rafts clusters in db/db mice

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Endothelium in spots : high-content imaging of lipid rafts clusters in db/db mice

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dc.contributor.author	Pilarczyk, Marta	pl
dc.contributor.author	Mateuszuk, Łukasz [SAP14003983]	pl
dc.contributor.author	Ryguła, Anna [SAP14002897]	pl
dc.contributor.author	Kępczyński, Mariusz [SAP11015411]	pl
dc.contributor.author	Chłopicki, Stefan [SAP20000969]	pl
dc.contributor.author	Barańska, Małgorzata [SAP11016906]	pl
dc.contributor.author	Kaczor, Agnieszka [SAP11018145]	pl
dc.date.accessioned	2015-06-12T06:22:53Z
dc.date.available	2015-06-12T06:22:53Z
dc.date.issued	2014	pl
dc.identifier.uri	http://ruj.uj.edu.pl/xmlui/handle/item/9476
dc.language	eng	pl
dc.rights	Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/pl/legalcode	*
dc.title	Endothelium in spots : high-content imaging of lipid rafts clusters in db/db mice	pl
dc.type	JournalArticle	pl
dc.identifier.weblink	https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0106065&type=printable	pl
dc.abstract.en	Lipid rafts (LRs) are dynamic, sterol- and sphingolipid-enriched nanodomains involved in the regulation of cellular functions and signal transduction, that upon stimuli, via (e.g. association of raft proteins and lipids), may cluster into domains of submicron or micron scale. Up to date, however, lipid raft clusters were observed only under artificially promoted conditions and their formation in vivo has not been confirmed. Using non-destructive approach involving Raman and Atomic Force Microscopy imaging we demonstrated the presence of clustered lipid rafts in endothelium of the aorta of the db/db mice that represent a reliable murine model of type 2 diabetes. The raft clusters in the aorta of diabetic mice were shown to occupy a considerably larger (about 10-fold) area of endothelial cells surface as compared to the control. Observation of pathology-promoted LRs confirms that the cellular increase of lipid content results in clustering of LRs. Clustering of LRs leads to the formation of assemblies with diameters up to 3 micrometers and increased lipid character. This massive clustering of lipid rafts in diabetes may trigger a signaling cascade leading to vascular inflammation.	pl
dc.description.volume	9	pl
dc.description.number	8	pl
dc.description.points	40	pl
dc.identifier.doi	10.1371/journal.pone.0106065	pl
dc.identifier.eissn	1932-6203	pl
dc.title.journal	PLoS ONE	pl
dc.language.container	eng	pl
dc.date.accession	2019-05-14	pl
dc.affiliation	Wydział Chemii : Zakład Fizyki Chemicznej	pl
dc.affiliation	Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii	pl
dc.affiliation	Pion Rektora : Jagiellońskie Centrum Rozwoju Leków	pl
dc.affiliation	Wydział Lekarski : Zakład Farmakologii Doświadczalnej	pl
dc.subtype	Article	pl
dc.identifier.articleid	e106065	pl
dc.rights.original	CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0;	pl
dc.identifier.project	ROD UJ / P	pl
dc.cm.id	68151
.pointsMNiSW	[2014 A]: 40