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Endothelium in spots : high-content imaging of lipid rafts clusters in db/db mice


Endothelium in spots : high-content imaging of lipid rafts clusters in db/db mice

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dc.contributor.author Pilarczyk, Marta pl
dc.contributor.author Mateuszuk, Łukasz [SAP14003983] pl
dc.contributor.author Ryguła, Anna [SAP14002897] pl
dc.contributor.author Kępczyński, Mariusz [SAP11015411] pl
dc.contributor.author Chłopicki, Stefan [SAP20000969] pl
dc.contributor.author Barańska, Małgorzata [SAP11016906] pl
dc.contributor.author Kaczor, Agnieszka [SAP11018145] pl
dc.date.accessioned 2015-06-12T06:22:53Z
dc.date.available 2015-06-12T06:22:53Z
dc.date.issued 2014 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/9476
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Endothelium in spots : high-content imaging of lipid rafts clusters in db/db mice pl
dc.type JournalArticle pl
dc.identifier.weblink https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0106065&type=printable pl
dc.abstract.en Lipid rafts (LRs) are dynamic, sterol- and sphingolipid-enriched nanodomains involved in the regulation of cellular functions and signal transduction, that upon stimuli, via (e.g. association of raft proteins and lipids), may cluster into domains of submicron or micron scale. Up to date, however, lipid raft clusters were observed only under artificially promoted conditions and their formation in vivo has not been confirmed. Using non-destructive approach involving Raman and Atomic Force Microscopy imaging we demonstrated the presence of clustered lipid rafts in endothelium of the aorta of the db/db mice that represent a reliable murine model of type 2 diabetes. The raft clusters in the aorta of diabetic mice were shown to occupy a considerably larger (about 10-fold) area of endothelial cells surface as compared to the control. Observation of pathology-promoted LRs confirms that the cellular increase of lipid content results in clustering of LRs. Clustering of LRs leads to the formation of assemblies with diameters up to 3 micrometers and increased lipid character. This massive clustering of lipid rafts in diabetes may trigger a signaling cascade leading to vascular inflammation. pl
dc.description.volume 9 pl
dc.description.number 8 pl
dc.description.points 40 pl
dc.identifier.doi 10.1371/journal.pone.0106065 pl
dc.identifier.eissn 1932-6203 pl
dc.title.journal PLoS ONE pl
dc.language.container eng pl
dc.date.accession 2019-05-14 pl
dc.affiliation Wydział Chemii : Zakład Fizyki Chemicznej pl
dc.affiliation Wydział Chemii : Zakład Chemii Fizycznej i Elektrochemii pl
dc.affiliation Pion Rektora : Jagiellońskie Centrum Rozwoju Leków pl
dc.affiliation Wydział Lekarski : Zakład Farmakologii Doświadczalnej pl
dc.subtype Article pl
dc.identifier.articleid e106065 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
dc.cm.id 68151
.pointsMNiSW [2014 A]: 40

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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa