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Is p53 controlling spermatogenesis in male mice with the deletion on the Y chromosome?


Is p53 controlling spermatogenesis in male mice with the deletion on the Y chromosome?

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dc.contributor.author Lech, Tomasz [USOS38887] pl
dc.contributor.author Gołas, Aniela [SAP11019122] pl
dc.contributor.author Styrna, Józefa [SAP11008587] pl
dc.date.accessioned 2015-06-08T13:22:09Z
dc.date.available 2015-06-08T13:22:09Z
dc.date.issued 2013 pl
dc.identifier.issn 0967-1994 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/9011
dc.language eng pl
dc.rights Dodaję tylko opis bibliograficzny *
dc.rights.uri *
dc.title Is p53 controlling spermatogenesis in male mice with the deletion on the Y chromosome? pl
dc.type JournalArticle pl
dc.description.physical 65-70 pl
dc.abstract.en The aim of the study was to evaluate the influence of the chromosome Y structure and Trp53 genotype on semen quality parameters. Mice with partial deletion of the Y chromosome (B10.BR-Y-del) have severely altered sperm head morphology when compared with males that possess the complete Y chromosome (B10.BR). Control males from B10.BR and B10.BR-Y-del mice, and mutant males from B10.BR-p53(-/-) and B10.BR-Y-del-p53(-/-) experimental groups were used. We assessed testis weight, sperm head abnormalities, viability of spermatozoa (eosin test), percentage of motile and immature sperm, and performed a hypo-osmotic test to detect abnormal tail membrane integrity. Sperm morphology and maturation were controlled by the genes within the deleted region of the Y chromosome. Testis weight was higher in the mutants than in the control males, possibly due to cell accumulation in Trp53-deficient males as the concentration of sperm was significantly increased in the mutants. An elevated percentage of abnormal sperm was noted in B10.BR-p53(-/-) and B10.BR-Y-del-p53(-/-) male mice. We suggest that, in Trp53-deficient mice, the sperm cells that escape apoptosis are the ones that have abnormal morphology. The only sperm quality parameter affected by the interplay between Trp53 and chromosome Y genes was sperm motility, which was elevated in B10.BR-p53(-/-) males, but remained unchanged in B10.BR-Y-del-p53(-/-) males. pl
dc.subject.en p53 pl
dc.subject.en semen quality pl
dc.subject.en Y-del pl
dc.subject.en sperm pl
dc.subject.en mice pl
dc.description.volume 21 pl
dc.description.number 1 pl
dc.identifier.doi 10.1017/s0967199411000323 pl
dc.identifier.eissn 1469-8730 pl
dc.title.journal Zygote pl
dc.language.container eng pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
dc.rights.original bez licencji pl
.pointsMNiSW [2013 A]: 15

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