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Is p53 controlling spermatogenesis in male mice with the deletion on the Y chromosome?
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Is p53 controlling spermatogenesis in male mice with the deletion on the Y chromosome?
Bibliographic description
| title: | Is p53 controlling spermatogenesis in male mice with the deletion on the Y chromosome? |
| author: | Lech Tomasz  , Gołas Aniela , Styrna Józefa
|
| journal title: | Zygote |
| volume: | 21 |
| issue: | 1 |
| date of publication : | 2013 |
| pages: | 65-70 |
| ISSN: |
0967-1994 |
| eISSN: |
1469-8730 |
| DOI: | |
| language: | English |
| journal language: | English |
| abstract in English: | The aim of the study was to evaluate the influence of the chromosome Y structure and Trp53 genotype on semen quality parameters. Mice with partial deletion of the Y chromosome (B10.BR-Y-del) have severely altered sperm head morphology when compared with males that possess the complete Y chromosome (B10.BR). Control males from B10.BR and B10.BR-Y-del mice, and mutant males from B10.BR-p53(-/-) and B10.BR-Y-del-p53(-/-) experimental groups were used. We assessed testis weight, sperm head abnormalities, viability of spermatozoa (eosin test), percentage of motile and immature sperm, and performed a hypo-osmotic test to detect abnormal tail membrane integrity. Sperm morphology and maturation were controlled by the genes within the deleted region of the Y chromosome. Testis weight was higher in the mutants than in the control males, possibly due to cell accumulation in Trp53-deficient males as the concentration of sperm was significantly increased in the mutants. An elevated percentage of abnormal sperm was noted in B10.BR-p53(-/-) and B10.BR-Y-del-p53(-/-) male mice. We suggest that, in Trp53-deficient mice, the sperm cells that escape apoptosis are the ones that have abnormal morphology. The only sperm quality parameter affected by the interplay between Trp53 and chromosome Y genes was sperm motility, which was elevated in B10.BR-p53(-/-) males, but remained unchanged in B10.BR-Y-del-p53(-/-) males. |
| keywords in English: | p53, semen quality, Y-del, sperm, mice |
| affiliation: | Wydział Biologii i Nauk o Ziemi : Instytut Zoologii |
| type: | journal article |
| subtype: | academic paper |
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Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
