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Effects of resistin on porcine ovarian follicle steroidogenesis in prepubertal animals : an in vitro study

Effects of resistin on porcine ovarian follicle ...

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dc.contributor.author Rak, Agnieszka [SAP11020106] pl
dc.contributor.author Durak-Kozica, Martyna [USOS78262] pl
dc.contributor.author Gregoraszczuk, Ewa [SAP11007273] pl
dc.date.accessioned 2015-06-02T18:47:46Z
dc.date.available 2015-06-02T18:47:46Z
dc.date.issued 2013 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/8751
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 2.0 *
dc.rights.uri http://creativecommons.org/licenses/by/2.0/pl/legalcode *
dc.title Effects of resistin on porcine ovarian follicle steroidogenesis in prepubertal animals : an in vitro study pl
dc.type JournalArticle pl
dc.description.additional Agnieszka Rak podpisana: Agnieszka Rak-Mardyła; Martyna Durak-Kozica podpisana: Martyna Durak pl
dc.abstract.en Background: Resistin was first reported to be an adipocyte-specific hormone, but recent studies have indicated a connection between resistin and reproductive function. However, it is not yet known if resistin is expressed by the ovary and if it can affect steroidogenesis in ovarian follicles from prepubertal pigs. Methods: In this study, using real time PCR, immunoblotting, and ELISA, we quantified resistin expression and concentration in maturing ovarian follicles (small, 3–4 mm; medium, 4–5 mm; large, 6–7 mm) collected from prepubertal pigs. In addition, the dose-responsive effects of recombinant human resistin (0.1, 1, 10, and 100 ng/ml) on steroid hormone (i.e., progesterone [P4], androstendione [A4], testosterone [T], and estradiol [E2]) secretion in culture medium and steroidogenic enzyme (i.e., CYP11A1, 3betaHSD, CYP17A1, 17betaHSD, and CYP19A1) expression in ovarian follicles were determined. Results: We observed that resistin gene and protein expression increased significantly (P < 0.05) during follicular growth, with large follicles expressing the highest level of this adipokine. Recombinant resistin also increased P4, A4, and T secretion by up-regulating the steady state levels of CYP11A1, 3betaHSD, CYP17A1, and 17betaHSD. Recombinant resistin had no effects on E2 secretion and CYP19A1 expression in ovarian follicles. Conclusion: Our results show resistin expression in ovarian follicles from prepubertal pigs for the first time. We also show that recombinant resistin stimulates steroidogenesis in ovarian follicles by increasing the expression of CYP11A1, 3betaHSD, CYP17A1, and 17betaHSD. The presence of resistin in the porcine ovary and its direct effects on steroidogenesis suggest that resistin is a new regulator of ovary function in prepubertal animals. pl
dc.subject.en resistin pl
dc.subject.en prepubertal porcine ovarian follicle pl
dc.subject.en steroidogenesis pl
dc.description.volume 11 pl
dc.identifier.doi 10.1186/1477-7827-11-45 pl
dc.identifier.eissn 1477-7827 pl
dc.title.journal Reproductive Biology and Endocrinology pl
dc.language.container eng pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
dc.identifier.articleid 45 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0 pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2013 A]: 20


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Udzielam licencji. Uznanie autorstwa 2.0 Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 2.0