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Analysis of the link between the redox state and enzymatic activity of the HtrA (DegP) protein from Escherichia coli

Analysis of the link between the redox state and ...

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dc.contributor.author Koper, Tomasz pl
dc.contributor.author Polit, Agnieszka [SAP11016972] pl
dc.contributor.author Sobiecka-Szkatula, Anna pl
dc.contributor.author Wegrzyn, Katarzyna pl
dc.contributor.author Scire, Andrea pl
dc.contributor.author Figaj, Donata pl
dc.contributor.author Kadzinski, Leszek pl
dc.contributor.author Zarzecka, Urszula pl
dc.contributor.author Zurawa-Janicka, Dorota pl
dc.contributor.author Banecki, Bogdan pl
dc.contributor.author Lesner, Adam pl
dc.contributor.author Tanfani, Fabio pl
dc.contributor.author Lipinska, Barbara pl
dc.contributor.author Skorko-Glonek, Joanna pl
dc.date.accessioned 2015-05-28T07:15:15Z
dc.date.available 2015-05-28T07:15:15Z
dc.date.issued 2015 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/8298
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Analysis of the link between the redox state and enzymatic activity of the HtrA (DegP) protein from Escherichia coli pl
dc.type JournalArticle pl
dc.abstract.en Bacterial HtrAs are proteases engaged in extracytoplasmic activities during stressful conditions and pathogenesis. A model prokaryotic HtrA (HtrA/DegP from Escherichia coli) requires activation to cleave its substrates efficiently. In the inactive state of the enzyme, one of the regulatory loops, termed LA, forms inhibitory contacts in the area of the active center. Reduction of the disulfide bond located in the middle of LA stimulates HtrA activity in vivo suggesting that this S-S bond may play a regulatory role, although the mechanism of this stimulation is not known. Here, we show that HtrA lacking an S-S bridge cleaved a model peptide substrate more efficiently and exhibited a higher affinity for a protein substrate. An LA loop lacking the disulfide was more exposed to the solvent; hence, at least some of the interactions involving this loop must have been disturbed. The protein without S-S bonds demonstrated lower thermal stability and was more easily converted to a dodecameric active oligomeric form. Thus, the lack of the disulfide within LA affected the stability and the overall structure of the HtrA molecule. In this study, we have also demonstrated that in vitro human thioredoxin 1 is able to reduce HtrA; thus, reduction of HtrA can be performed enzymatically. pl
dc.description.volume 10 pl
dc.description.number 2 pl
dc.identifier.doi 10.1371/journal.pone.0117413 pl
dc.identifier.eissn 1932-6203 pl
dc.title.journal PLoS ONE pl
dc.language.container eng pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznej pl
dc.subtype Article pl
dc.identifier.articleid e0117413 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2015 A]: 40

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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa