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Determination of desipramine and nortriptyline in blood by means of the HPLC-DAD method using 7,7,8,8-tetracyanoquinodimethane (TCNQ) as a derivatisation agent
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Na tych samych warunkach 4.0 Międzynarodowa
Determination of desipramine and nortriptyline in blood by means of the HPLC-DAD method using 7,7,8,8-tetracyanoquinodimethane (TCNQ) as a derivatisation agent
Bibliographic description
| title: | Determination of desipramine and nortriptyline in blood by means of the HPLC-DAD method using 7,7,8,8-tetracyanoquinodimethane (TCNQ) as a derivatisation agent |
| alternative title: |
Oznaczenie dezypraminy i nortryptyliny we krwi metodą HPLC-DAD z zastosowaniem 7,7,8,8-tetracjanochinodimetanu (TCNQ) jako odczynnika derywatyzującego |
| author: | Woźniakiewicz Michał  , Kuczara Jagoda, Kościelniak Paweł
|
| journal title: | Problems of Forensic Sciences = Z Zagadnień Nauk Sądowych |
| volume: | 69 |
| date of publication : | 2007 |
| pages: | 90-97 |
| ISSN: |
1230-7483 |
| URL: | |
| date accessed: |
2019-09-16 |
| notes: | Art. w 2 wersjach językowych: tekst w jęz. ang. (s. 90-94) i pol. (s. 95-97). Bibliogr. s. 94. |
| language: | English |
| journal language: | English |
| abstract in English: | The aim of present study was to develop a sensitive and reliable method of simultaneous determination of desipramine and nortriptyline in blood by means of high-performance liquid chromatography coupled with a diode array spectrophotometer (HPLC-DAD). For this purpose, a procedure based on formation of colour derivatives of these drugs with 7,7,8,8-tetrat cyanoquinodimethane (TCNQ) was proposed. The tested drugs and the internal standard were added to blood samples, and then they were extracted by the microwave assisted liquid-liquid extraction method. The separated organic layer was evaporated and was subjected to derivatisation by TCNQ reagent dissolved in acetonitrile. Method validation was performed and the limits of detection (0.03-0.05 pg/ml) and quantitation (0.09-0.18 pg/ml) ofboth analytes were determined, as were the precision and accuracy of their determination at two concentration levels. The worked-out method altows determination of desipramine and nortriptyline present in blood at therapeutic levels. |
| keywords in English: | desipramine, nortriptyline, TCNQ, HPLC-DAD, derivatisation |
| affiliation: | Wydział Chemii : Zakład Chemii Analitycznej |
| type: | journal article |
| subtype: | academic paper |
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Na tych samych warunkach 4.0 Międzynarodowa
Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
