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The nucleocapsid protein of human coronavirus NL63

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The nucleocapsid protein of human coronavirus NL63

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dc.contributor.author Zuwała, Kaja pl
dc.contributor.author Golda, Anna [SAP13037861] pl
dc.contributor.author Kabala, Wojciech pl
dc.contributor.author Burmistrz, Michał [USOS77743] pl
dc.contributor.author Zdżalik, Michał [USOS11798] pl
dc.contributor.author Nowak, Paulina [USOS155319] pl
dc.contributor.author Kędracka-Krok, Sylwia [SAP11016010] pl
dc.contributor.author Zarębski, Mirosław [SAP11019075] pl
dc.contributor.author Dobrucki, Jerzy [SAP11010251] pl
dc.contributor.author Florek, Dominik pl
dc.contributor.author Zeglen, Slawomir pl
dc.contributor.author Wojarski, Jacek pl
dc.contributor.author Potempa, Jan [SAP11010833] pl
dc.contributor.author Dubin, Grzegorz [SAP11019243] pl
dc.contributor.author Pyrć, Krzysztof [SAP11019543] pl
dc.date.accessioned 2015-05-27T11:44:14Z
dc.date.available 2015-05-27T11:44:14Z
dc.date.issued 2015 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/8251
dc.language eng pl
dc.rights Udzielam licencji. Przekazanie do Domeny Publicznej 1.0 *
dc.rights.uri http://creativecommons.org/publicdomain/zero/1.0/legalcode.pl *
dc.title The nucleocapsid protein of human coronavirus NL63 pl
dc.type JournalArticle pl
dc.abstract.en Human coronavirus (HCoV) NL63 was first described in 2004 and is associated with respiratory tract disease of varying severity. At the genetic and structural level, HCoV-NL63 is similar to other members of the Coronavirinae subfamily, especially human coronavirus 229E (HCoV-229E). Detailed analysis, however, reveals several unique features of the pathogen. The coronaviral nucleocapsid protein is abundantly present in infected cells. It is a multi-domain, multi-functional protein important for viral replication and a number of cellular processes. The aim of the present study was to characterize the HCoV-NL63 nucleocapsid protein. Biochemical analyses revealed that the protein shares characteristics with homologous proteins encoded in other coronaviral genomes, with the N-terminal domain responsible for nucleic acid binding and the C-terminal domain involved in protein oligomerization. Surprisingly, analysis of the subcellular localization of the N protein of HCoV-NL63 revealed that, differently than homologous proteins from other coronaviral species except for SARS-CoV, it is not present in the nucleus of infected or transfected cells. Furthermore, no significant alteration in cell cycle progression in cells expressing the protein was observed. This is in stark contrast with results obtained for other coronaviruses, except for the SARS-CoV. pl
dc.description.volume 10 pl
dc.description.number 2 pl
dc.identifier.doi 10.1371/journal.pone.0117833 pl
dc.identifier.eissn 1932-6203 pl
dc.title.journal PLoS ONE pl
dc.language.container eng pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Mikrobiologii pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biochemii Fizycznej pl
dc.affiliation Pion Prorektora ds. badań naukowych i funduszy strukturalnych : Małopolskie Centrum Biotechnologii pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Pracownia Biofizyki Komórki pl
dc.subtype Article pl
dc.identifier.articleid e0117833 pl
dc.rights.original OTHER; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2015 A]: 40


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Udzielam licencji. Przekazanie do Domeny Publicznej 1.0 Except where otherwise noted, this item's license is described as Udzielam licencji. Przekazanie do Domeny Publicznej 1.0