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Phosphoinositides play differential roles in regulating phototropin1- and phototropin2-mediated chloroplast movements in Arabidopsis

Phosphoinositides play differential roles in regulating ...

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dc.contributor.author Aggarwal, Chhavi [SAP11020233] pl
dc.contributor.author Łabuz, Justyna [SAP13005009] pl
dc.contributor.author Gabryś, Halina [SAP11007886] pl
dc.date.accessioned 2015-05-15T12:33:01Z
dc.date.available 2015-05-15T12:33:01Z
dc.date.issued 2013 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/7184
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa *
dc.rights.uri https://creativecommons.org/licenses *
dc.title Phosphoinositides play differential roles in regulating phototropin1- and phototropin2-mediated chloroplast movements in Arabidopsis pl
dc.type JournalArticle pl
dc.abstract.en Phototropins are UVA/blue-light receptors involved in controlling the light-dependent physiological responses which serve to optimize the photosynthetic activity of plants and promote growth. The phototropin-induced phosphoinositide (PI) metabolism has been shown to be essential for stomatal opening and phototropism. However, the role of PIs in phototropin-induced chloroplast movements remains poorly understood. The aim of this work is to determine which PI species are involved in the control of chloroplast movements in Arabidopsis and the nature of their involvement. We present the effects of the inactivation of phospholipase C (PLC), PI3-kinase (PI3K) and PI4-kinase (PI4K) on chloroplast relocations in Arabidopsis. The inhibition of the phosphatidylinositol 4,5-bisphospahte [PI(4,5)P2]-PLC pathway, using neomycin and U73122, suppressed the phot2-mediated chloroplast accumulation and avoidance responses, without affecting movement responses controlled by phot1. On the other hand, PI3K and PI4K activities are more restricted to phot1- and phot2-induced weak-light responses. The inactivation of PI3K and PI4K by wortmannin and LY294002 severely affected the weak blue-light-activated accumulation response but had little effect on the strong blue-light-activated avoidance response. The inhibitory effect observed with PI metabolism inhibitors is, at least partly, due to a disturbance in {{Ca^2^+}_(_c_)} signaling. Using the transgenic aequorin system, we show that the application of these inhibitors suppresses the blue-light-induced transient {{Ca^2^+}_(_c_)} rise. These results demonstrate the importance of PIs in chloroplast movements, with the PI(4,5)P2-PLC pathway involved in phot2 signaling while PI3K and PI4K are required for the phot1- and phot2-induced accumulation response. Our results suggest that these PIs modulate cytosolic {Ca^2^+} signaling during movements. pl
dc.description.volume 8 pl
dc.description.number 2 pl
dc.identifier.doi 10.1371/journal.pone.0055393 pl
dc.identifier.eissn 1932-6203 pl
dc.title.journal PLoS ONE pl
dc.language.container eng pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Roślin pl
dc.subtype Article pl
dc.identifier.articleid e55393 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2013 A]: 40


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Udzielam licencji. Uznanie autorstwa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa