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Development, validation and application of a micro-liquid chromatography-tandem mass spectrometry based method for simultaneous quantification of selected protein biomarkers of endothelial dysfunction in murine plasma

Development, validation and application of a ...

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dc.contributor.author Suraj, Joanna [SAP14011732] pl
dc.contributor.author Kurpińska, Anna [SAP14017395] pl
dc.contributor.author Olkowicz, Mariola pl
dc.contributor.author Niedzielska-Andres, Ewa [SAP20014304] pl
dc.contributor.author Smolik, Magdalena [SAP20017303] pl
dc.contributor.author Zakrzewska, Agnieszka [SAP14003150] pl
dc.contributor.author Jasztal, Agnieszka [SAP14006898] pl
dc.contributor.author Sitek, Barbara [SAP14005869] pl
dc.contributor.author Chłopicki, Stefan [SAP14004240] pl
dc.contributor.author Walczak, Maria [SAP14003398] pl
dc.date.accessioned 2019-03-23T07:41:09Z
dc.date.available 2019-03-23T07:41:09Z
dc.date.issued 2018 pl
dc.identifier.issn 0731-7085 pl
dc.identifier.uri https://ruj.uj.edu.pl/xmlui/handle/item/71143
dc.language eng pl
dc.rights Dodaję tylko opis bibliograficzny *
dc.rights.uri *
dc.title Development, validation and application of a micro-liquid chromatography-tandem mass spectrometry based method for simultaneous quantification of selected protein biomarkers of endothelial dysfunction in murine plasma pl
dc.type JournalArticle pl
dc.description.physical 465-474 pl
dc.abstract.en The objective of this study was to develop and validate the method based on micro-liquid chromatography-tandem mass spectrometry (microLC/MS-MRM) for simultaneous determination of adiponectin (ADN), von Willebrand factor (vWF), soluble form of vascular cell adhesion molecule 1 (sVCAM-1), soluble form of intercellular adhesion molecule 1 (sICAM-1) and syndecan-1 (SDC-1) in mouse plasma. The calibration range was established from 2.5pmol/mL to 5000pmol/mL for ADN; 5pmol/mL to 5000pmol/mL for vWF; 0.375pmol/mL to 250pmol/mL for sVCAM-1 and sICAM-1; and 0.25pmol/mL to 250pmol/mL for SDC-1. The method was applied to measure the plasma concentration of selected proteins in mice fed high-fat diet (HFD), and revealed the pro-thrombotic status by increased concentration of vWF (1.31±0.17 nmol/mL (Control) vs 1.98±0.09 nmol/mL (HFD), p <0.05) and the dysregulation of adipose tissue metabolism by decreased concentration of ADN (0.62±0.08 nmol/mL (Control) vs 0.37±0.06 nmol/mL (HFD), p <0.05). In conclusion, the microLC/MS-MRM-based method allows for reliable measurements of selected protein biomarkers of endothelial dysfunction in mouse plasma. pl
dc.subject.pl biomarker pl
dc.subject.pl śródbłonek pl
dc.subject.pl dysfunkcja pl
dc.subject.pl dieta bogato-tłuszczowa pl
dc.subject.pl LC/MS-MRM pl
dc.subject.pl myszy pl
dc.subject.en biomarker pl
dc.subject.en endothelium pl
dc.subject.en dysfunction pl
dc.subject.en high-fat diet pl
dc.subject.en LC/MS-MRM pl
dc.subject.en mice pl
dc.description.volume 149 pl
dc.identifier.doi 10.1016/j.jpba.2017.11.023 pl
dc.identifier.eissn 1873-264X pl
dc.title.journal Journal of Pharmaceutical and Biomedical Analysis pl
dc.language.container eng pl
dc.affiliation Pion Prorektora ds. badań naukowych i funduszy strukturalnych : Jagiellońskie Centrum Rozwoju Leków pl
dc.affiliation Wydział Farmaceutyczny : Zakład Toksykologii pl
dc.subtype Article pl
dc.rights.original bez licencji pl
dc.identifier.project ROD UJ / O pl
.pointsMNiSW [2018 A]: 35


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