Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants
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dc.type
JournalArticle
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dc.description.physical
67-73
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dc.abstract.en
Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.
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dc.subject.en
callogenesis
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dc.subject.en
extracellular matrix
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dc.subject.en
in vitro culture
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dc.subject.en
scanning electron microscopy
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dc.subject.en
topinambur
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dc.subject.en
transmission electron microscopy
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dc.description.volume
83
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dc.description.number
1
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dc.identifier.doi
10.5586/asbp.2014.009
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dc.identifier.eissn
2083-9480
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dc.title.journal
Acta Societatis Botanicorum Poloniae
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dc.language.container
eng
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dc.affiliation
Wydział Biologii i Nauk o Ziemi : Instytut Botaniki
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dc.subtype
Article
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dc.rights.original
CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0;