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Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants
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Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants
Bibliographic description
| title: | Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants |
| author: | Pilarska Maria  , Popielarska-Konieczna Marzena , Ślesak Halina , Kozieradzka-Kiszkurno Małgorzata, Góralski Grzegorz , Konieczny Robert , Bohdanowicz Jerzy, Kuta Elżbieta
|
| journal title: | Acta Societatis Botanicorum Poloniae |
| volume: | 83 |
| issue: | 1 |
| date of publication : | 2014 |
| pages: | 67-73 |
| ISSN: |
0001-6977 |
| eISSN: |
2083-9480 |
| DOI: | |
| language: | English |
| journal language: | English |
| abstract in English: | Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar. |
| keywords in English: | callogenesis, extracellular matrix, in vitro culture, scanning electron microscopy, topinambur, transmission electron microscopy |
| affiliation: | Wydział Biologii i Nauk o Ziemi : Instytut Botaniki |
| type: | journal article |
| subtype: | academic paper |
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Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 3.0
Projekt „Repozytorium otwartego dostępu do dorobku naukowego i dydaktycznego UJ” współfinansowany w ramach poddziałania 2.3.1 „Cyfrowe udostępnianie zasobów nauki” Programu Operacyjnego Polska Cyfrowa z Europejskiego Funduszu Rozwoju Regionalnego i budżetu państwa na podstawie umowy o dofinansowanie nr POPC.02.03.01-00-0030/17-00
