From April 2nd to April 5th, 2024, works related to the implementation of the new version of the Jagiellonian University Repository system will be carried out. It will not be possible to enter new information into the repository during this time. We apologize for any inconvenience.
Where it’s at really matters : "In situ in vivo" vascular endothelial growth factor spatially correlates with electron paramagnetic resonance pO_{2} images in tumors of living mice
Where it’s at really matters : "In situ in vivo" vascular endothelial growth factor spatially correlates with electron paramagnetic resonance pO_{2} images in tumors of living mice
Where it’s at really matters : "In situ in vivo" vascular endothelial growth factor spatially correlates with electron paramagnetic resonance pO_{2} images in tumors of living mice
author:
Elas Martyna , Hleihel Danielle, Barth Eugene D., Haney Chad R., Ahn Kang-Hyun, Pelizzari Charles A., Epel Boris, Weichselbaum Ralph R., Halpern Howard J.
Purpose: Tumor microenvironments show remarkable tumor pO_{2} heterogeneity, as seen in prior EPR pO_{2} images (EPROI). pO_{2} correlation with hypoxia response proteins is frustrated by large rapid pO2 changes with position.
Procedures: To overcome this limitation, biopsies stereotactically located in the EPROI were used to explore the relationship between vascular endothelial growth factor A (VEGF) concentrations in living mouse tumors and the local EPROI pO_{2}.
Results: Quantitative ELISA VEGF concentrations correlated (p = 0.0068 to 0.019) with mean pO_{2}, median pO_{2}, and the fraction of voxels in the biopsy volume with pO_{2} less than 3, 6, and 10 Torr.
Conclusions: This validates EPROI hypoxic fractions at the molecular level and provides a new paradigm for the assessment of the relationship, in vivo, between hypoxia and hypoxia response proteins. When translated to human subjects, this will enhance understanding of human tumor pathophysiology and cancer response to therapy.