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How does the sweet violet (Viola odorata L.) fight pathogens and pests : cyclotides as a comprehensive plant host defense system


How does the sweet violet (Viola odorata L.) fight pathogens and pests : cyclotides as a comprehensive plant host defense system

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dc.contributor.author Slazak, Blazej pl
dc.contributor.author Kapusta, Małgorzata pl
dc.contributor.author Strömstedt, Adam A. pl
dc.contributor.author Słomka, Aneta [SAP11020210] pl
dc.contributor.author Krychowiak, Marta pl
dc.contributor.author Shariatgorji, Mohammadreza pl
dc.contributor.author Andrén, Per E. pl
dc.contributor.author Bohdanowicz, Jerzy pl
dc.contributor.author Kuta, Elżbieta [SAP11007367] pl
dc.contributor.author Göransson, Ulf pl
dc.date.accessioned 2018-09-19T07:10:18Z
dc.date.available 2018-09-19T07:10:18Z
dc.date.issued 2018 pl
dc.identifier.uri https://ruj.uj.edu.pl/xmlui/handle/item/57139
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title How does the sweet violet (Viola odorata L.) fight pathogens and pests : cyclotides as a comprehensive plant host defense system pl
dc.type JournalArticle pl
dc.abstract.en Cyclotides are cyclic plant polypeptides of 27-37 amino acid residues. They have been extensively studied in bioengineering and drug development contexts. However, less is known about the relevance of cyclotides for the plants producing them. The anti-insect larvae effects of kB1 and antibacterial activity of cyO2 suggest that cyclotides are a part of plant host defense. The sweet violet (Viola odorata L.) produces a wide array of cyclotides, including kB1 (kalata B1) and cyO2 (cycloviolacin O2), with distinct presumed biological roles. Here, we evaluate V. odorata cyclotides’ potency against plant pathogens and their mode of action using bioassays, liposome experiments and immunogold labeling for transmission electron microscopy (TEM). We explore the link between the biological activity and distribution in plant generative, vegetative tissues and seeds, depicted by immunohistochemistry and matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). Cyclotides cyO2, cyO3, cyO13, and cyO19 are shown to have potent activity against model fungal plant pathogens (Fusarium oxysporum, F. graminearum, F. culmorum, Mycosphaerella fragariae, Botrytis cinerea) and fungi isolated from violets (Colletotrichum utrechtense and Alternaria alternata), with minimal inhibitory concentrations (MICs) ranging from 0.8 to 25 mM. Inhibition of phytopathogenic bacteria - Pseudomonas syringae pv. syringae, Dickeya dadantii and Pectobacterium atrosepticum - is also observed with MIC = 25-100 mM. A membrane-disrupting antifungal mode of action is shown. Finding cyO2 inside the fungal spore cells in TEM images may indicate that other, intracellular targets may be involved in the mechanism of toxicity. Fungi can not break down cyclotides in the course of days. varv A (kalata S) and kB1 show little potency against pathogenic fungi when compared with the tested cycloviolacins. cyO2, cyO3, cyO19 and kB1 are differentially distributed and found in tissues vulnerable to pathogen (epidermis, rizodermis, vascular bundles, protodermis, procambium, ovary walls, outer integuments) and pest (ground tissues of leaf and petiole) attacks, respectively, indicating a link between the cyclotides’ sites of accumulation and biological role. Cyclotides emerge as a comprehensive defense system in V. odorata, in which different types of peptides have specific targets that determine their distribution in plant tissues. pl
dc.subject.en cyclotides pl
dc.subject.en plant host defense pl
dc.subject.en Violaceae pl
dc.subject.en antimicrobial peptide pl
dc.subject.en antifungal defense pl
dc.subject.en MALDI-MSI pl
dc.subject.en immunohistochemistry pl
dc.description.volume 9 pl
dc.identifier.doi 10.3389/fpls.2018.01296 pl
dc.identifier.eissn 1664-462X pl
dc.title.journal Frontiers in Plant Science pl
dc.language.container eng pl
dc.affiliation Wydział Biologii : Instytut Botaniki pl
dc.subtype Article pl
dc.identifier.articleid 1296 pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0 pl
dc.identifier.project ROD UJ / OP pl
.pointsMNiSW [2018 A]: 40

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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa