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Blue-light-activated phototropin2 trafficking from the cytoplasm to Golgi/post-Golgi vesicles

Blue-light-activated phototropin2 trafficking from ...

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dc.contributor.author Aggarwal, Chhavi [SAP11020233] pl
dc.contributor.author Banaś, Agnieszka [SAP11019078] pl
dc.contributor.author Kasprowicz-Maluśki, Anna pl
dc.contributor.author Borghetti, Carolina [SAP14010012] pl
dc.contributor.author Łabuz, Justyna [SAP13005009] pl
dc.contributor.author Dobrucki, Jerzy [SAP11010251] pl
dc.contributor.author Gabryś, Halina [SAP11007886] pl
dc.date.accessioned 2015-04-24T07:07:52Z
dc.date.available 2015-04-24T07:07:52Z
dc.date.issued 2014 pl
dc.identifier.issn 0022-0957 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/5625
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 3.0 Polska *
dc.rights.uri http://creativecommons.org/licenses/by/3.0/pl/legalcode *
dc.title Blue-light-activated phototropin2 trafficking from the cytoplasm to Golgi/post-Golgi vesicles pl
dc.type JournalArticle pl
dc.description.physical 3263-3276 pl
dc.description.additional Bibliogr. s. 3275-3276 pl
dc.abstract.en Phototropins are plasma membrane-localized UVA/blue light photoreceptors which mediate phototropism, inhibition of primary hypocotyl elongation, leaf positioning, chloroplast movements, and stomatal opening. Blue light irradiation activates the C-terminal serine/threonine kinase domain of phototropin which autophosphorylates the receptor. Arabidopsis thaliana encodes two phototropins, phot1 and phot2. In response to blue light, phot1 moves from the plasma membrane into the cytosol and phot2 translocates to the Golgi complex. In this study the molecular mechanism and route of blue-light-induced phot2 trafficking are demonstrated. It is shown that Atphot2 behaves in a similar manner when expressed transiently under 35S or its native promoter. The phot2 kinase domain but not blue-light-mediated autophosphorylation is required for the receptor translocation. Using co-localization and western blotting, the receptor was shown to move from the cytoplasm to the Golgi complex, and then to the post-Golgi structures. The results were confirmed by brefeldin A (an inhibitor of the secretory pathway) which disrupted phot2 trafficking. An association was observed between phot2 and the light chain2 of clathrin via bimolecular fluorescence complementation. The fluorescence was observed at the plasma membrane. The results were confirmed using co-immunoprecipitation. However, tyrphostin23 (an inhibitor of clathrin-mediated endocytosis) and wortmannin (a suppressor of receptor endocytosis) were not able to block phot2 trafficking, indicating no involvement of receptor endocytosis in the formation of phot2 punctuate structures. Protein turnover studies indicated that the receptor was continuously degraded in both darkness and blue light. The degradation of phot2 proceeded via a transport route different from translocation to the Golgi complex. pl
dc.subject.en Arabidopsis thaliana pl
dc.subject.en trafficking pl
dc.subject.en phototropin pl
dc.subject.en Golgi complex pl
dc.subject.en degradation pl
dc.subject.en blue light pl
dc.description.volume 65 pl
dc.description.number 12 pl
dc.identifier.doi 10.1093/jxb/eru172 pl
dc.identifier.eissn 1460-2431 pl
dc.title.journal Journal of Experimental Botany pl
dc.language.container eng pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Zakład Biotechnologii Roślin pl
dc.affiliation Wydział Biochemii, Biofizyki i Biotechnologii : Pracownia Biofizyki Komórki pl
dc.subtype Article pl
dc.rights.original CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0; pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2014 A]: 45


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Udzielam licencji. Uznanie autorstwa 3.0 Polska Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 3.0 Polska