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Hexachlorobenzene and pentachlorobenzene accumulation, metabolism and effect on steroid secretion and on CYP11A1 and CYP19 expression in cultured human placental tissue

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Hexachlorobenzene and pentachlorobenzene accumulation, metabolism and effect on steroid secretion and on CYP11A1 and CYP19 expression in cultured human placental tissue

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dc.contributor.author Gregoraszczuk, Ewa [SAP11007273] pl
dc.contributor.author Ptak, Anna [SAP11019184] pl
dc.contributor.author Karpeta, Anna [USOS12449] pl
dc.contributor.author Fiedor, Elżbieta [USOS97494] pl
dc.contributor.author Wróbel, Anna Maria [SAP14003078] pl
dc.contributor.author Milewicz, T. [SAP20001252] pl
dc.contributor.author Falandysz, J. pl
dc.date.accessioned 2015-04-21T10:26:18Z
dc.date.available 2015-04-21T10:26:18Z
dc.date.issued 2014 pl
dc.identifier.issn 0890-6238 pl
dc.identifier.uri http://ruj.uj.edu.pl/xmlui/handle/item/5374
dc.language eng pl
dc.rights Dodaję tylko opis bibliograficzny *
dc.rights.uri *
dc.title Hexachlorobenzene and pentachlorobenzene accumulation, metabolism and effect on steroid secretion and on CYP11A1 and CYP19 expression in cultured human placental tissue pl
dc.type JournalArticle pl
dc.description.physical 102-110 pl
dc.abstract.en Hexachlorobenzene and pentachlorobenzene accumulation and the effect on CYP1A1, SULT1A, COMT and steroid secretion in term placental tissue were determined. Explants of placental tissue were exposed to between 0.02 and 2 ng/ml HCBz or PeCBz for 6-72 h. Accumulation was measured by capillary gas chromatography and quadrupole mass spectrometry. CYP1A1, SULT1A, COMT activity and progesterone secretion were analysed by EIA. Protein expression was quantified by Western blot; 6% HCBz and 7% PeCBz were detected in the tissue. Fast induction of CYP1A1 activity and protein expression in the presence of HCBz were observed. HCBz increased, while PeCBz decreased COMT protein expression. The stimulatory effect of HCBz, and the inhibitory of PeCBz on progesterone secretion and CYP11A1 protein expression were noted. Later activation of CYP1A1, inhibition of COMT protein expression and progesterone secretion by PeCBz suggest greater exposure to PeCBz and pointing at PeCBz as the main factor responsible for the disruption of placental function. pl
dc.subject.en hexachlorobenzene pl
dc.subject.en pentachlorobenzene pl
dc.subject.en placenta pl
dc.subject.en steroidogenesis pl
dc.description.volume 43 pl
dc.identifier.doi 10.1016/j.reprotox.2013.12.004 pl
dc.identifier.eissn 1873-1708 pl
dc.title.journal Reproductive Toxicology pl
dc.language.container eng pl
dc.affiliation Wydział Lekarski : Klinika Endokrynologii Ginekologicznej pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Zoologii pl
dc.subtype Article pl
dc.rights.original bez licencji pl
dc.cm.id 62426
.pointsMNiSW [2014 A]: 30


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