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Female versus male : Rumex thyrsiflorus Fingerh. under in vitro conditions : does sex influence in vitro morphogenesis?


Female versus male : Rumex thyrsiflorus Fingerh. under in vitro conditions : does sex influence in vitro morphogenesis?

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dc.contributor.author Ślesak, Halina [SAP11116278] pl
dc.contributor.author Dziedzic, Katarzyna [USOS95024] pl
dc.contributor.author Kwolek, Dagmara [SAP13905165] pl
dc.contributor.author Cygan, Magdalena [USOS112081] pl
dc.contributor.author Mizia, Patryk [USOS38956] pl
dc.contributor.author Olejniczak, Paweł pl
dc.contributor.author Joachimiak, Andrzej [SAP11008588] pl
dc.date.accessioned 2018-02-09T12:28:35Z
dc.date.available 2018-02-09T12:28:35Z
dc.date.issued 2017 pl
dc.identifier.issn 0167-6857 pl
dc.identifier.uri https://ruj.uj.edu.pl/xmlui/handle/item/49972
dc.language eng pl
dc.rights Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/pl/legalcode *
dc.title Female versus male : Rumex thyrsiflorus Fingerh. under in vitro conditions : does sex influence in vitro morphogenesis? pl
dc.type JournalArticle pl
dc.description.physical 521-532 pl
dc.abstract.en Rumex thyrsiflorus Fingerh. is a dioecious plant with polymorphic sex chromosomes (XX in females, XY_{1}Y_{2} in males). This species is an interesting plant for study on the structure and function of sex chromosomes, the sex ratio, and is also a valuable source of bioactive constituents. A procedure for the micropropagation of R. thyrsiflorus from hypocotyl explants, which can be useful for analyses of sex-specific morphogenetic reactions under in vitro conditions, was developed. The molecular analysis, based on genetic sex markers, allowed the sex of explants to be determined in order to create male or female in vitro cultures. Regeneration primarily occurred via indirect adventitious shoot formation as well as via somatic embryogenesis, which was confirmed by histological and scanning electron microscope (SEM) analyses. The highest frequency of explants that revealed a morphogenetic response with the highest number of regenerated adventitious shoot buds (average 11.5 shoots per explant) was obtained on a Murashige and Skoog (MS) medium supplemented with 2.27 \mu M thidiazuron (TDZ). Molecular analysis revealed a female-biased sex ratio under these conditions. The relationship between the sex of the explants and their morphogenetic potential was studied. The efficiency of morphogenesis varied between the two sexes and depended on the ratio of auxin and cytokinin in the medium. The germination pattern of male and female seeds under in vitro conditions was also analysed. No differences in germination time between male and female seeds and in terms of male and female seedling length were observed, which indicated that there was no competition between the sexes during seed germination when grown under uniform growth conditions. pl
dc.subject.en organogenesis pl
dc.subject.en somatic embryogenesis pl
dc.subject.en histological analysis pl
dc.subject.en scanning electron microscopy pl
dc.subject.en sex ratio pl
dc.subject.en genetic sex marker pl
dc.description.volume 129 pl
dc.description.number 3 pl
dc.identifier.doi 10.1007/s11240-017-1197-4 pl
dc.identifier.eissn 1573-5044 pl
dc.title.journal Plant Cell, Tissue and Organ Culture pl
dc.language.container eng pl
dc.affiliation Wydział Biologii i Nauk o Ziemi : Instytut Botaniki pl
dc.subtype Article pl
dc.rights.original CC-BY; inne; ostateczna wersja wydawcy; w momencie opublikowania; 0 pl
dc.identifier.project ROD UJ / P pl
.pointsMNiSW [2017 A]: 35

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Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa Except where otherwise noted, this item's license is described as Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa