Precise mass determination of single cell with cantilever-based microbiosensor system

doi:10.1371/journal.pone.0188388

Precise mass determination of single cell with cantilever-based microbiosensor system

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Precise mass determination of single cell with cantilever-based microbiosensor system

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dc.contributor.author	Łabędź, Bogdan [USOS113502]	pl
dc.contributor.author	Wańczyk, Aleksandra [USOS114926]	pl
dc.contributor.author	Rajfur, Zenon [SAP14007955]	pl
dc.date.accessioned	2017-12-12T12:20:41Z
dc.date.available	2017-12-12T12:20:41Z
dc.date.issued	2017	pl
dc.identifier.uri	https://ruj.uj.edu.pl/xmlui/handle/item/47544
dc.language	eng	pl
dc.rights	Udzielam licencji. Uznanie autorstwa 4.0 Międzynarodowa	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/pl/legalcode	*
dc.title	Precise mass determination of single cell with cantilever-based microbiosensor system	pl
dc.type	JournalArticle	pl
dc.abstract.en	Having determined the mass of a single cell of brewer yeast Saccharomyces cerevisiae by means of a microcantilever-based biosensor Cantisens CSR-801 (Concentris, Basel, Switzerland), it was found that its dry mass is 47,65 $\pm$ 1,05 pg. Found to be crucial in this mass determination was the cell position along the length of the cantilever. Moreover, calculations including cells positions on the cantilever provide a threefold better degree of accuracy than those which assume uniform mass distribution. We have also examined the influence of storage time on the single cell mass. Our results show that after 6 months there is an increase in the average mass of a single yeast cell.	pl
dc.description.volume	12	pl
dc.description.number	11	pl
dc.identifier.doi	10.1371/journal.pone.0188388	pl
dc.identifier.eissn	1932-6203	pl
dc.title.journal	PLoS ONE	pl
dc.language.container	eng	pl
dc.affiliation	Wydział Fizyki, Astronomii i Informatyki Stosowanej : Instytut Fizyki im. Mariana Smoluchowskiego	pl
dc.subtype	Article	pl
dc.identifier.articleid	e0188388	pl
dc.rights.original	CC-BY; otwarte czasopismo; ostateczna wersja wydawcy; w momencie opublikowania; 0	pl
dc.identifier.project	ROD UJ / P	pl
.pointsMNiSW	[2017 A]: 40